Detailed information of component protein


Summary

Component ID T6CP000107
Component protein type TssM
T6SS ID (Type) T6SS00015 experimental (Type i1)
Strain Pseudomonas putida KT2440
Replicon chromosome
Sequence Protein sequence (1209 a.a.); Nucleotide sequence (3627 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PP_RS21140
Coordinate (Strand) 4596182..4599808 (-)
NCBI ID WP_010954863.1
RefSeq NC_002947
Uniprot ID Q88FL8_PSEPK, A0A2V4F7Q2_9PSED
KEGG ID ppu:PP_4071
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 7.9e-71 527..831
IcmF-related_N PF14331.8 9e-65 220..475
IcmF_C PF06744.14 4.3e-23 1066..1170
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-18 MNQLWKYLKRWGWPLMTR
Transmembrane helix 19-41 IGLAMPLLLGLGAVLMLVAIWWL
Outside 42-55 GPQWTWRDQQPLAS
Transmembrane helix 56-78 VAHRSMASLVFVLVALLSWLVVL
Inside 79-469 RTRFRRLQAERQQAMAAEVDPTQPFVHAQEKALSQGLARYLDNAGGRRALYRLPWYLVLGARQAGKSSFIDCTDQSFSLTRIDKAQARGRPAQALAYPVGWWISNDAVIIDPPGAFISQNGPADSLGSDSTDVESSVPSGTQAKLWQHLLDWLQRNRSQRALNGLVLVVDLPALLHGTVEQRTALAHLLRTRLYEVSSQLGSRLPLYVVLSKFDLLDGFDQFYSKLSAAKRNSLFGFTFKLDAVDTFDAWLGEYGEHYDRLLEQLFEQVIDRLDVQGSPALRSRLYSLHRQLLGLRPMLLSFLRETLASDRFTTPALVRGVYWSSVVQHGDVRNAFVREAAQPYKTSLPLLEGKPQAKALAYFIQQAFGRVIYKEAGLAGDNVRVARRKRQ
Transmembrane helix 470-489 LLWVGSSVGVLAFCVAIASW
Outside 490-1208 HRYFDINGVKAASVLAKSREYSHHEVDQRLDPTGRNLLEPLDQIRDAVAVFGDYRAAWPGVADLGLYQGRTIGPTVDEAYLSLLSRRFLPALASGVIEAMDAAPPGSEQQMAALRVYRMLEDRRNRRAEWVEDWMARQWQQAFPGQGQLQRDLMRHLKYALTYADTDLPQYRQRVSHVQQTLRKVPLPQRVYAGLKQQAQEQLHTGLDLRHQVGPAFDVVYQLSSGSSQGDNGVLLAPMLTAKGFKEYFEPRSQRFTEMAMIDQWALGERAQLDYSDADQAALNERLRNLYSADYIDSWRRALNAFSVADFRDLDHGVTILEQLAGPAAPLHRLLETVRDNSSLLSPVNVGVADEAPSPVSINSKPEQQQALVIQRAFAGLSAMLHAAGEKPSYYDETHAAIVAVHDYAKAVQGSPDRGKAALHAVHQRFSMTGHDPIGTLQRVATGLPEPINHHVRKVAHQTAQVLNVEALRELERRWDAEVYSFFQQRLAERYPFVVRAPDASLEDFEAFFGPNGRLQQFQDQYLKLFLKDNLEALQNGLQGRSLIRTDVIEQLERADRIRETFFDQRGNLSVQFSIEPLGLSANQRTSLLDLDGQLISYTHGPSQITGIVWPNTLGQHVRSNLTLLRQNGNSSSLEYRGPWSMFRLLSRGALNGRTATSVDLSFKTGDGVMRYRLNAEKAFNPITQQPFKGFRLPRGLLQQPVRVALAEQADVPVM


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9920 - -


  Protein sequence: 1209 a.a.    

>T6CP000107 NC_002947:c4599808-4596182 [Pseudomonas putida KT2440] [TssM]
MNQLWKYLKRWGWPLMTRIGLAMPLLLGLGAVLMLVAIWWLGPQWTWRDQQPLASVAHRSMASLVFVLVALLSWLVVLRT
RFRRLQAERQQAMAAEVDPTQPFVHAQEKALSQGLARYLDNAGGRRALYRLPWYLVLGARQAGKSSFIDCTDQSFSLTRI
DKAQARGRPAQALAYPVGWWISNDAVIIDPPGAFISQNGPADSLGSDSTDVESSVPSGTQAKLWQHLLDWLQRNRSQRAL
NGLVLVVDLPALLHGTVEQRTALAHLLRTRLYEVSSQLGSRLPLYVVLSKFDLLDGFDQFYSKLSAAKRNSLFGFTFKLD
AVDTFDAWLGEYGEHYDRLLEQLFEQVIDRLDVQGSPALRSRLYSLHRQLLGLRPMLLSFLRETLASDRFTTPALVRGVY
WSSVVQHGDVRNAFVREAAQPYKTSLPLLEGKPQAKALAYFIQQAFGRVIYKEAGLAGDNVRVARRKRQLLWVGSSVGVL
AFCVAIASWHRYFDINGVKAASVLAKSREYSHHEVDQRLDPTGRNLLEPLDQIRDAVAVFGDYRAAWPGVADLGLYQGRT
IGPTVDEAYLSLLSRRFLPALASGVIEAMDAAPPGSEQQMAALRVYRMLEDRRNRRAEWVEDWMARQWQQAFPGQGQLQR
DLMRHLKYALTYADTDLPQYRQRVSHVQQTLRKVPLPQRVYAGLKQQAQEQLHTGLDLRHQVGPAFDVVYQLSSGSSQGD
NGVLLAPMLTAKGFKEYFEPRSQRFTEMAMIDQWALGERAQLDYSDADQAALNERLRNLYSADYIDSWRRALNAFSVADF
RDLDHGVTILEQLAGPAAPLHRLLETVRDNSSLLSPVNVGVADEAPSPVSINSKPEQQQALVIQRAFAGLSAMLHAAGEK
PSYYDETHAAIVAVHDYAKAVQGSPDRGKAALHAVHQRFSMTGHDPIGTLQRVATGLPEPINHHVRKVAHQTAQVLNVEA
LRELERRWDAEVYSFFQQRLAERYPFVVRAPDASLEDFEAFFGPNGRLQQFQDQYLKLFLKDNLEALQNGLQGRSLIRTD
VIEQLERADRIRETFFDQRGNLSVQFSIEPLGLSANQRTSLLDLDGQLISYTHGPSQITGIVWPNTLGQHVRSNLTLLRQ
NGNSSSLEYRGPWSMFRLLSRGALNGRTATSVDLSFKTGDGVMRYRLNAEKAFNPITQQPFKGFRLPRGLLQQPVRVALA
EQADVPVM*

  Nucleotide sequence: 3627 bp    

>T6CP000107 NC_002947:c4599808-4596182 [Pseudomonas putida KT2440] [TssM]
ATGAATCAACTCTGGAAGTACCTCAAACGTTGGGGCTGGCCGCTGATGACCCGTATCGGTCTGGCAATGCCGCTGCTACT
TGGGCTTGGCGCTGTGCTGATGCTGGTCGCCATCTGGTGGCTCGGCCCGCAATGGACGTGGCGCGATCAGCAGCCGTTGG
CCAGTGTGGCGCATCGTAGTATGGCCAGCCTGGTGTTCGTGTTGGTGGCGCTGTTGAGCTGGCTGGTTGTCTTGAGAACG
CGCTTTCGTCGCTTGCAAGCAGAACGTCAGCAGGCGATGGCTGCCGAGGTGGATCCGACGCAACCGTTCGTTCATGCACA
AGAGAAGGCGTTGAGCCAAGGGCTCGCACGCTACCTGGACAATGCCGGTGGGCGTCGCGCGCTGTACCGATTGCCGTGGT
ACCTCGTGCTGGGCGCCAGGCAGGCCGGCAAGAGCAGCTTTATTGACTGCACCGATCAAAGTTTCTCGTTGACCCGCATC
GACAAGGCGCAGGCACGGGGCCGGCCAGCGCAGGCGCTCGCCTACCCCGTGGGGTGGTGGATCAGCAATGATGCGGTGAT
CATCGACCCGCCCGGTGCCTTTATCAGCCAGAACGGCCCCGCAGATTCGCTGGGCAGTGACTCCACTGACGTTGAGTCTT
CGGTACCGTCTGGAACCCAAGCCAAACTGTGGCAGCATCTGTTGGATTGGCTGCAGCGCAATCGAAGCCAGAGAGCCCTC
AACGGCCTGGTGCTGGTTGTCGACCTGCCTGCGCTACTGCACGGTACCGTCGAGCAACGCACGGCCCTTGCCCACCTGCT
GCGCACACGTCTTTACGAGGTGAGCAGCCAGTTGGGCTCGCGCTTGCCGCTGTATGTCGTGTTGAGCAAGTTCGATCTGC
TGGACGGCTTCGACCAATTCTACAGCAAGCTTTCGGCCGCCAAGCGCAATAGCCTGTTTGGATTCACGTTCAAGCTGGAT
GCTGTCGACACCTTCGACGCTTGGCTGGGCGAGTATGGCGAACACTACGATCGGTTGCTGGAGCAGCTGTTCGAGCAGGT
GATCGATCGTCTGGACGTGCAGGGTAGCCCTGCGTTGCGTTCACGTCTCTACTCACTGCATAGGCAGTTGCTCGGGTTGC
GTCCAATGCTGTTGAGTTTTCTGCGCGAGACCTTGGCAAGTGACCGTTTCACCACCCCGGCGCTGGTGCGCGGTGTGTAT
TGGTCGTCAGTCGTACAGCACGGTGACGTGCGCAACGCCTTTGTCCGTGAGGCGGCGCAGCCTTACAAGACCAGCCTCCC
GTTGCTTGAGGGCAAGCCGCAGGCCAAGGCGTTGGCCTACTTCATTCAACAGGCCTTTGGGCGGGTCATCTACAAAGAGG
CCGGCCTTGCCGGCGATAACGTCAGGGTGGCTCGGCGCAAGCGCCAACTGCTGTGGGTGGGTTCTTCTGTAGGCGTTCTG
GCCTTCTGCGTCGCCATCGCCAGCTGGCATCGTTACTTCGACATCAATGGCGTCAAGGCCGCCAGCGTGCTGGCCAAGAG
CCGGGAGTACAGCCATCACGAAGTGGATCAGCGTCTGGACCCGACCGGGCGCAACTTGCTGGAGCCGCTGGACCAGATCC
GCGATGCGGTAGCAGTGTTTGGCGACTACCGCGCTGCGTGGCCGGGTGTTGCTGATCTGGGGCTGTACCAGGGCCGTACC
ATCGGGCCGACCGTGGATGAGGCGTACCTCAGCTTGTTGTCCAGGCGCTTCTTGCCTGCGTTGGCCAGCGGCGTGATCGA
GGCCATGGATGCGGCGCCCCCCGGCAGCGAGCAGCAAATGGCCGCGCTCCGGGTTTACCGGATGCTTGAGGATCGCCGGA
ACCGTCGAGCGGAATGGGTCGAGGACTGGATGGCGCGACAGTGGCAACAGGCGTTCCCCGGCCAGGGGCAGCTGCAACGA
GACCTCATGCGGCACCTGAAGTATGCGCTGACCTACGCGGATACCGACCTGCCGCAGTATCGCCAGCGTGTCAGCCACGT
GCAGCAGACATTGCGTAAGGTGCCGTTGCCACAACGCGTGTATGCGGGCCTGAAGCAGCAGGCCCAGGAGCAGTTGCATA
CCGGTCTGGACCTGCGTCATCAGGTGGGGCCTGCGTTCGATGTCGTTTACCAGCTGTCTTCCGGCTCGAGCCAGGGCGAC
AATGGTGTTTTGCTGGCGCCAATGCTCACTGCCAAAGGCTTCAAGGAGTACTTTGAACCGCGTAGTCAGCGCTTTACGGA
AATGGCCATGATCGACCAGTGGGCACTGGGGGAACGTGCTCAACTGGATTACTCCGATGCCGACCAGGCTGCGCTGAACG
AGCGCCTGCGCAACCTGTACAGCGCCGACTACATCGACAGCTGGCGCCGCGCCTTGAACGCATTCTCCGTGGCGGATTTT
CGAGACCTGGATCATGGCGTCACGATCCTTGAACAGTTGGCCGGGCCAGCGGCGCCGTTGCACCGATTGCTGGAGACGGT
TCGCGACAACAGTTCGCTGTTATCACCGGTTAATGTTGGCGTGGCGGATGAGGCCCCTTCGCCCGTTTCGATCAACAGCA
AGCCTGAGCAACAACAGGCGCTGGTCATTCAGCGGGCGTTTGCAGGCTTGAGCGCCATGTTGCACGCTGCAGGCGAAAAA
CCGAGCTACTACGATGAAACCCACGCAGCCATAGTCGCCGTCCACGACTATGCCAAGGCGGTACAGGGTAGCCCAGACCG
AGGCAAGGCTGCGCTGCACGCAGTGCATCAACGCTTTTCAATGACCGGGCACGACCCCATCGGCACCTTGCAACGCGTTG
CCACTGGCTTGCCGGAGCCAATCAATCACCACGTTAGAAAGGTGGCACATCAAACGGCACAGGTGCTGAACGTTGAAGCG
CTGCGCGAGTTGGAGCGGCGATGGGATGCTGAAGTCTACAGCTTCTTCCAGCAGCGCCTGGCAGAGCGCTACCCTTTCGT
AGTCAGGGCACCCGACGCTTCGCTGGAAGACTTTGAAGCGTTCTTCGGGCCGAATGGGCGACTGCAGCAGTTCCAGGATC
AATATCTGAAGCTTTTCCTCAAGGACAATCTGGAAGCCTTGCAGAATGGTTTGCAGGGGCGATCCCTGATCCGTACCGAT
GTGATCGAGCAGCTGGAACGGGCCGATCGCATCCGCGAAACGTTCTTTGATCAGCGCGGTAACCTCAGCGTGCAGTTCAG
TATCGAGCCGTTGGGGCTTAGTGCAAATCAACGCACCAGCCTGCTGGATCTGGATGGCCAGCTGATTTCCTACACCCACG
GCCCAAGCCAGATCACCGGTATTGTCTGGCCCAATACCTTGGGGCAACACGTGCGCAGCAACCTGACCCTGCTCAGGCAG
AACGGCAATAGCAGCAGCCTGGAGTATCGAGGGCCTTGGTCGATGTTTCGCCTGCTGAGCCGCGGTGCGCTCAATGGGCG
TACGGCAACCAGCGTGGACCTGAGCTTCAAAACAGGTGATGGTGTGATGCGCTACCGGCTGAACGCGGAAAAGGCCTTCA
ATCCTATTACTCAACAACCGTTCAAAGGTTTTCGGTTGCCCCGAGGGTTGTTGCAACAGCCAGTCAGGGTGGCGTTGGCC
GAACAGGCGGATGTGCCTGTCATGTAG