SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000102
Component protein type	TssF
T6SS ID (Type)	T6SS00014 (Type i4b)
Strain	Pseudomonas putida KT2440
Replicon	chromosome
Sequence	Protein sequence (607 a.a.); Nucleotide sequence (1821 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [3] Bernal P, Allsopp LP, Filloux A, et al. The Pseudomonas putida T6SS is a plant warden against phytopathogens. ISME J. 2017 Apr;11(4):972-987. doi: 10.1038/ismej.2016.169. Epub 2017 Jan 3. PMID: 28045455

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PP_RS16040
Coordinate (Strand)	3492382..3494202 (-)
NCBI ID	WP_010953993.1
RefSeq	NC_002947
Uniprot ID	Q88IA2_PSEPK, A0A2V4FG99_9PSED
KEGG ID	ppu:PP_3097
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	2.5e-216	1..603

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-606	MLDELLPYYEKELSHLRFLGQEFAAQYPKIASRLLIEGDNCEDPHTERLIEAFSFLSARVHKKLDDEFPEIVESFLEVLYPHYLRPTPSMSIVELSLGRHEKVTEAYRVARHTEMHANPVEGVVCKFRTCYPVELWPIAVQQASFTEMERSAFNGHSADLVARLRIRLAATGDALFGQMDMNRLRFFLDGEATLMLQLYELLFNNLAKATLSFDDQGRSREVALPADALKTVGYARDEGLVDYSERSFLGYRLLHEYFTFPDKFMFFDLSGFARILQGKAIEQVEVNFYFSDYDLSERLARLTQNIGRNNFKLNCTPIINLFRQQAEPIKLTHTQHEYQVTPDTRLHNAAEVVSIDRVRRVRKLGGIDQVATCQPFFEPRGEQDPHSSFWVARRRNQGDATAMSIRVVDRDLELIDASSDTLSIGLTCSNRDVPLMLPFGGERGDFTIPANSVIKDIRCLRKPTATVRVPLGKGLIWRLIAHLSLNHLSLVSQGREVLLELLSLYNYRNVSAIRKQINGIKAISSEPVVARIGHPRPNFVRGVGITLTLDESQFIGSGVFLFGRVLDHFFGQYCSMNSFTQLTLRTQQREKRVVQWPARTGDQPLV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9957	-	-

Protein sequence: 607 a.a.

						>T6CP000102 NC_002947:c3494202-3492382 [Pseudomonas putida KT2440] [TssF]
MLDELLPYYEKELSHLRFLGQEFAAQYPKIASRLLIEGDNCEDPHTERLIEAFSFLSARVHKKLDDEFPEIVESFLEVLY
PHYLRPTPSMSIVELSLGRHEKVTEAYRVARHTEMHANPVEGVVCKFRTCYPVELWPIAVQQASFTEMERSAFNGHSADL
VARLRIRLAATGDALFGQMDMNRLRFFLDGEATLMLQLYELLFNNLAKATLSFDDQGRSREVALPADALKTVGYARDEGL
VDYSERSFLGYRLLHEYFTFPDKFMFFDLSGFARILQGKAIEQVEVNFYFSDYDLSERLARLTQNIGRNNFKLNCTPIIN
LFRQQAEPIKLTHTQHEYQVTPDTRLHNAAEVVSIDRVRRVRKLGGIDQVATCQPFFEPRGEQDPHSSFWVARRRNQGDA
TAMSIRVVDRDLELIDASSDTLSIGLTCSNRDVPLMLPFGGERGDFTIPANSVIKDIRCLRKPTATVRVPLGKGLIWRLI
AHLSLNHLSLVSQGREVLLELLSLYNYRNVSAIRKQINGIKAISSEPVVARIGHPRPNFVRGVGITLTLDESQFIGSGVF
LFGRVLDHFFGQYCSMNSFTQLTLRTQQREKRVVQWPARTGDQPLV*					

Nucleotide sequence: 1821 bp

						>T6CP000102 NC_002947:c3494202-3492382 [Pseudomonas putida KT2440] [TssF]
ATGCTCGACGAACTGCTGCCCTACTACGAAAAGGAACTGTCGCACCTGCGCTTTCTCGGCCAAGAGTTTGCTGCGCAGTA
CCCGAAGATCGCTTCGCGGTTGCTGATCGAGGGCGACAACTGCGAAGACCCGCACACCGAGCGCCTGATCGAGGCGTTCT
CGTTCCTGTCTGCGCGGGTGCACAAGAAGCTCGATGACGAGTTTCCGGAAATCGTCGAATCCTTCCTCGAGGTACTGTAC
CCCCACTACCTGCGCCCTACTCCGTCGATGTCGATTGTCGAACTGAGCCTGGGCAGGCACGAAAAAGTCACCGAAGCCTA
CCGCGTCGCGCGCCATACCGAGATGCATGCCAATCCCGTGGAAGGCGTGGTGTGCAAGTTCCGCACCTGCTACCCGGTAG
AGCTGTGGCCGATTGCCGTGCAGCAGGCCTCGTTCACAGAAATGGAACGCTCGGCCTTCAACGGGCACAGCGCCGACCTG
GTCGCTCGTCTGCGCATCCGCCTGGCAGCCACTGGCGATGCCCTGTTCGGGCAGATGGACATGAACCGCCTGCGGTTCTT
CCTCGACGGCGAAGCGACCCTGATGCTGCAGCTGTACGAACTGCTGTTCAACAACCTCGCCAAGGCCACGCTGAGCTTCG
ACGACCAGGGCCGCAGCCGCGAGGTGGCGCTGCCGGCCGATGCCCTGAAAACCGTGGGCTATGCCCGTGACGAAGGCCTG
GTGGATTACTCCGAACGCTCATTCCTGGGCTACCGCCTGCTGCACGAGTATTTCACCTTCCCTGACAAGTTCATGTTCTT
CGACCTCAGTGGATTTGCCCGCATCCTGCAGGGCAAGGCCATCGAGCAGGTAGAGGTCAACTTCTATTTCTCCGACTACG
ACCTCAGCGAGCGCCTGGCGCGGCTGACCCAGAACATCGGGCGCAACAACTTCAAGCTCAACTGCACGCCGATCATCAAC
CTGTTCCGCCAGCAGGCCGAGCCAATCAAGCTGACCCACACCCAGCACGAATACCAGGTCACCCCTGACACACGGCTGCA
CAACGCTGCAGAAGTGGTATCGATCGACCGGGTACGGCGGGTGCGCAAACTCGGCGGTATCGACCAGGTCGCCACCTGCC
AGCCGTTTTTCGAACCCCGGGGCGAGCAAGACCCACACAGCAGCTTCTGGGTTGCGCGCCGGCGAAACCAGGGTGACGCC
ACTGCCATGAGCATCCGCGTGGTCGACCGCGATCTGGAACTGATCGACGCCAGCAGCGACACCCTCAGTATCGGCCTTAC
CTGCAGCAACCGCGACGTGCCGCTGATGCTGCCGTTCGGTGGCGAACGGGGCGATTTCACCATTCCCGCCAACTCGGTGA
TCAAGGATATCCGCTGCCTGCGCAAGCCCACCGCCACGGTGCGTGTGCCCTTGGGCAAGGGCCTGATCTGGCGGTTGATC
GCCCACCTGTCGCTGAACCATCTGTCGCTGGTCAGCCAGGGCCGCGAAGTGCTGCTGGAGCTGTTGTCGCTGTACAACTA
CCGCAATGTCTCCGCCATTCGCAAACAGATCAACGGCATCAAGGCGATCAGCAGCGAGCCGGTGGTCGCGCGCATCGGCC
ACCCGCGGCCAAATTTCGTGCGCGGCGTCGGCATCACCCTGACCCTGGACGAAAGCCAGTTCATCGGCAGCGGCGTGTTT
CTGTTCGGCAGGGTGCTGGACCACTTCTTCGGCCAGTACTGCTCGATGAACAGTTTCACCCAACTGACCCTGCGCACCCA
ACAACGAGAAAAGAGAGTCGTACAATGGCCAGCACGCACCGGCGATCAGCCCCTGGTCTGA					