Detailed information of component protein
Summary
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | PP_RS16025 |
| Coordinate (Strand) | 3488007..3488729 (-) |
| NCBI ID | WP_004576638.1 |
| RefSeq | NC_002947 |
| Uniprot ID | A0A2I0CH72_9PSED, Q88IA5_PSEPK, A0A2K4LQ44_9PSED, I3V3X0_PSEPU, A0A4U9M678_PSEPU, A5W3Q2_PSEP1, A0A2V4FBR6_9PSED, A0A7L9GD41_9PSED |
| KEGG ID | ppi:YSA_10461, ppf:Pput_2628, ppu:PP_3094 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 4.2e-30 | 47..161 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-240 | MQHKHIVLKRLTAAALLVALAGCGVTDRIGKRMEDSWAADMLADSEKVILTSDGGNQLNPDAEGKPLSVVMRVYQLTDLERFAATDAETLWDAPQKALGNTLVETREITLLPGIGQIDQWPLAKNARYVGVAAFFRDEQDARWKVAFDANSLRKDGIWFSSDGLRVLVDNTEISAMRGVDVLNKPPTADQLAAAQKAKDADATLTDKVQDAAIDKATDAASQSASNAMDSTFNSLVDSVK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9986 | pos: 22-23. LAG-CG. | MQHKHIVLKRLTAAALLVALAG |
Protein sequence: 241 a.a.
>T6CP000099 NC_002947:c3488729-3488007 [Pseudomonas putida KT2440] [TssJ]
MQHKHIVLKRLTAAALLVALAGCGVTDRIGKRMEDSWAADMLADSEKVILTSDGGNQLNPDAEGKPLSVVMRVYQLTDLE
RFAATDAETLWDAPQKALGNTLVETREITLLPGIGQIDQWPLAKNARYVGVAAFFRDEQDARWKVAFDANSLRKDGIWFS
SDGLRVLVDNTEISAMRGVDVLNKPPTADQLAAAQKAKDADATLTDKVQDAAIDKATDAASQSASNAMDSTFNSLVDSVK
*
MQHKHIVLKRLTAAALLVALAGCGVTDRIGKRMEDSWAADMLADSEKVILTSDGGNQLNPDAEGKPLSVVMRVYQLTDLE
RFAATDAETLWDAPQKALGNTLVETREITLLPGIGQIDQWPLAKNARYVGVAAFFRDEQDARWKVAFDANSLRKDGIWFS
SDGLRVLVDNTEISAMRGVDVLNKPPTADQLAAAQKAKDADATLTDKVQDAAIDKATDAASQSASNAMDSTFNSLVDSVK
*
Nucleotide sequence: 723 bp
>T6CP000099 NC_002947:c3488729-3488007 [Pseudomonas putida KT2440] [TssJ]
ATGCAACACAAGCACATCGTCCTCAAACGCCTGACCGCAGCCGCCTTGCTGGTAGCGCTGGCCGGCTGCGGGGTCACCGA
CCGTATCGGCAAGCGCATGGAAGACAGCTGGGCGGCCGACATGCTGGCCGACAGCGAAAAGGTCATCCTGACCTCCGACG
GCGGCAACCAGCTCAACCCCGACGCCGAGGGCAAGCCGCTGTCGGTGGTGATGCGCGTGTATCAGCTGACCGACCTCGAG
CGCTTCGCGGCTACCGATGCCGAGACCCTGTGGGACGCCCCGCAAAAAGCCTTGGGCAACACCCTGGTGGAAACCCGCGA
GATCACCCTGCTGCCCGGCATTGGCCAGATCGACCAGTGGCCGCTGGCCAAGAACGCGCGCTACGTCGGCGTGGCGGCGT
TCTTCCGCGATGAGCAGGACGCGCGCTGGAAAGTCGCCTTCGACGCCAACTCGCTGCGCAAGGACGGTATCTGGTTCTCC
TCCGACGGCCTGCGGGTACTGGTGGACAACACCGAAATCAGCGCCATGCGGGGCGTGGACGTACTGAACAAACCGCCGAC
CGCCGACCAGCTTGCCGCTGCGCAGAAGGCCAAGGATGCCGACGCGACCCTCACCGACAAGGTGCAGGACGCGGCCATCG
ACAAAGCCACCGACGCCGCCAGCCAGTCGGCCAGCAACGCCATGGATTCAACCTTCAACTCTTTAGTGGATAGCGTCAAA
TGA
ATGCAACACAAGCACATCGTCCTCAAACGCCTGACCGCAGCCGCCTTGCTGGTAGCGCTGGCCGGCTGCGGGGTCACCGA
CCGTATCGGCAAGCGCATGGAAGACAGCTGGGCGGCCGACATGCTGGCCGACAGCGAAAAGGTCATCCTGACCTCCGACG
GCGGCAACCAGCTCAACCCCGACGCCGAGGGCAAGCCGCTGTCGGTGGTGATGCGCGTGTATCAGCTGACCGACCTCGAG
CGCTTCGCGGCTACCGATGCCGAGACCCTGTGGGACGCCCCGCAAAAAGCCTTGGGCAACACCCTGGTGGAAACCCGCGA
GATCACCCTGCTGCCCGGCATTGGCCAGATCGACCAGTGGCCGCTGGCCAAGAACGCGCGCTACGTCGGCGTGGCGGCGT
TCTTCCGCGATGAGCAGGACGCGCGCTGGAAAGTCGCCTTCGACGCCAACTCGCTGCGCAAGGACGGTATCTGGTTCTCC
TCCGACGGCCTGCGGGTACTGGTGGACAACACCGAAATCAGCGCCATGCGGGGCGTGGACGTACTGAACAAACCGCCGAC
CGCCGACCAGCTTGCCGCTGCGCAGAAGGCCAAGGATGCCGACGCGACCCTCACCGACAAGGTGCAGGACGCGGCCATCG
ACAAAGCCACCGACGCCGCCAGCCAGTCGGCCAGCAACGCCATGGATTCAACCTTCAACTCTTTAGTGGATAGCGTCAAA
TGA