Detailed information of component protein


Summary

Component ID T6CP000098
Component protein type TssK
T6SS ID (Type) T6SS00014 experimental (Type i4b)
Strain Pseudomonas putida KT2440
Replicon chromosome
Sequence Protein sequence (448 a.a.); Nucleotide sequence (1344 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PP_RS16020
Coordinate (Strand) 3486667..3488010 (-)
NCBI ID WP_010953991.1
RefSeq NC_002947
Uniprot ID Q88IA6_PSEPK, A0A2V4FNK9_9PSED
KEGG ID ppu:PP_3093
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasE PF05936.14 3.7e-136 19..445
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-447 MSKQSRVMWSEGMFLLPQHFQYQDEFHQHQLAEATLRSTPFHWGVQALQVDEDALANGSLQLKRLKLVFPDGSLYDAPQHDPLPAARDLKDLLKANDLKVYAALKLPEPFGLNYVEDGHEHKAARRFRKQFDTLPDLNEGELENEITSLRLNVVLLVDGDSLDGYSHCPLAKLTRNSMGGFNLDPHFVHPTLHLGSHETIAGIGKRLLGALQAKSKALSGRRRERADQIAEFGSSDVTLFWLLNTVNRAHPQLAHLLAHPRLHPERLYLFLADLAGGLLTFTLDTQLSDIPEYDHHDPAASLVKLDEMIRVMLDNVVPNQCIVINLTQTKPSHWQGQLHDPRLAEADFYIAVHADMPGASLLELVPRAFKVGSPEDIEVVVNSAMPGVTLNHAARLPNAIPVRLDNQYFAIEPHGTVYERMISAQAICFYAPSAFTNLKLELMAVLK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9924 - -


  Protein sequence: 448 a.a.    

>T6CP000098 NC_002947:c3488010-3486667 [Pseudomonas putida KT2440] [TssK]
MSKQSRVMWSEGMFLLPQHFQYQDEFHQHQLAEATLRSTPFHWGVQALQVDEDALANGSLQLKRLKLVFPDGSLYDAPQH
DPLPAARDLKDLLKANDLKVYAALKLPEPFGLNYVEDGHEHKAARRFRKQFDTLPDLNEGELENEITSLRLNVVLLVDGD
SLDGYSHCPLAKLTRNSMGGFNLDPHFVHPTLHLGSHETIAGIGKRLLGALQAKSKALSGRRRERADQIAEFGSSDVTLF
WLLNTVNRAHPQLAHLLAHPRLHPERLYLFLADLAGGLLTFTLDTQLSDIPEYDHHDPAASLVKLDEMIRVMLDNVVPNQ
CIVINLTQTKPSHWQGQLHDPRLAEADFYIAVHADMPGASLLELVPRAFKVGSPEDIEVVVNSAMPGVTLNHAARLPNAI
PVRLDNQYFAIEPHGTVYERMISAQAICFYAPSAFTNLKLELMAVLK*

  Nucleotide sequence: 1344 bp    

>T6CP000098 NC_002947:c3488010-3486667 [Pseudomonas putida KT2440] [TssK]
ATGAGCAAGCAGAGCCGGGTGATGTGGTCGGAAGGCATGTTCCTGCTGCCCCAGCACTTCCAGTATCAGGACGAATTCCA
TCAGCACCAGTTGGCTGAGGCGACCCTGCGCAGCACCCCTTTCCATTGGGGGGTTCAGGCGTTGCAGGTGGACGAGGACG
CCCTGGCCAATGGTTCACTGCAGCTCAAGCGCCTGAAGCTGGTGTTCCCCGATGGCAGCCTCTACGACGCGCCGCAGCAC
GATCCGCTGCCGGCCGCGCGCGACCTCAAGGACCTGCTCAAGGCCAACGACCTGAAGGTCTATGCTGCGCTCAAGCTGCC
CGAGCCATTCGGCCTCAACTACGTCGAGGATGGCCACGAACACAAGGCCGCACGGCGTTTCCGCAAGCAGTTCGACACCC
TGCCCGACCTTAATGAAGGCGAGCTGGAGAACGAAATCACCAGCTTGCGCCTGAACGTCGTACTGCTGGTCGACGGTGAC
AGCCTCGACGGTTACAGCCACTGCCCTCTGGCCAAGCTGACACGCAACAGCATGGGCGGTTTCAACCTTGACCCGCATTT
CGTCCACCCCACCTTGCACCTGGGCAGCCACGAGACCATTGCCGGCATCGGCAAACGCCTGCTCGGCGCCCTGCAGGCCA
AGAGCAAGGCGCTTTCCGGCCGGCGCCGCGAGCGCGCCGACCAGATCGCCGAGTTCGGCTCCAGCGATGTAACCCTGTTC
TGGCTGCTGAACACGGTCAACCGTGCCCACCCGCAGCTGGCTCATCTACTGGCCCACCCGCGCCTGCACCCAGAGCGCCT
GTACCTGTTCCTGGCCGACCTTGCCGGCGGCCTGCTGACCTTCACCCTGGACACCCAGCTCAGCGACATCCCCGAGTACG
ACCACCACGACCCGGCCGCGTCGCTGGTCAAGCTCGACGAAATGATCCGGGTGATGCTCGACAACGTCGTGCCCAACCAG
TGCATTGTCATCAACCTGACCCAGACCAAGCCATCGCACTGGCAAGGCCAACTGCACGACCCGCGCCTGGCCGAAGCCGA
CTTCTATATCGCCGTGCACGCCGACATGCCCGGCGCCAGCCTGCTGGAGCTGGTGCCCCGTGCCTTCAAGGTCGGTTCGC
CGGAAGACATTGAAGTGGTGGTCAACAGCGCCATGCCGGGTGTCACCCTCAATCATGCCGCGCGGCTGCCGAACGCGATT
CCGGTGCGCCTGGACAACCAGTACTTCGCCATCGAGCCACACGGCACTGTGTATGAGCGAATGATCAGCGCCCAGGCCAT
CTGCTTCTATGCGCCCAGCGCCTTTACCAACCTCAAGCTTGAACTGATGGCGGTGCTCAAATGA