Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ABC803_RS01195 Genome accession   NZ_CP155531
Coordinates   224098..225114 (+) Length   338 a.a.
NCBI ID   WP_013193332.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain SN36255     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 219098..230114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABC803_RS01175 (ABC803_01175) nagA 220245..221396 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  ABC803_RS01180 (ABC803_01180) - 221559..222617 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  ABC803_RS01185 (ABC803_01185) - 222768..223133 (+) 366 WP_000286415.1 DUF1033 family protein -
  ABC803_RS01190 (ABC803_01190) comGA/cglA/cilD 223209..224150 (+) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  ABC803_RS01195 (ABC803_01195) comGB/cglB 224098..225114 (+) 1017 WP_013193332.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABC803_RS01200 (ABC803_01200) comGC/cglC 225116..225442 (+) 327 WP_000738650.1 comG operon protein ComGC Machinery gene
  ABC803_RS01205 (ABC803_01205) comGD/cglD 225435..225839 (+) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABC803_RS01210 (ABC803_01210) comGE/cglE 225802..226104 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABC803_RS01215 (ABC803_01215) comGF/cglF 226067..226528 (+) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABC803_RS01220 (ABC803_01220) comGG/cglG 226506..226919 (+) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABC803_RS01225 (ABC803_01225) - 227056..227643 (+) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  ABC803_RS01230 (ABC803_01230) comYH 227704..228657 (+) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  ABC803_RS01235 (ABC803_01235) - 228708..229898 (+) 1191 WP_000167766.1 acetate kinase -
  ABC803_RS01240 (ABC803_01240) - 229899..230030 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38524.60 Da        Isoelectric Point: 9.6162

>NTDB_id=997003 ABC803_RS01195 WP_013193332.1 224098..225114(+) (comGB/cglB) [Streptococcus pneumoniae strain SN36255]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=997003 ABC803_RS01195 WP_013193332.1 224098..225114(+) (comGB/cglB) [Streptococcus pneumoniae strain SN36255]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TAACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTTTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae D39

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae R6

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae TIGR4

99.112

100

0.991
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment