Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EL018_RS00335 Genome accession   NZ_LR134082
Coordinates   71138..71806 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli strain NCTC9038     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 66138..76806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL018_RS00305 (NCTC9038_00064) yicC 66158..67021 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  EL018_RS00310 (NCTC9038_00065) rph 67148..67864 (+) 717 WP_001247093.1 ribonuclease PH -
  EL018_RS00315 (NCTC9038_00066) pyrE 67929..68570 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EL018_RS00320 (NCTC9038_00067) slmA 68607..69203 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EL018_RS00325 (NCTC9038_00068) dut 69310..69768 (-) 459 WP_000976070.1 dUTP diphosphatase -
  EL018_RS00330 (NCTC9038_00069) coaBC 69746..70966 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EL018_RS00335 (NCTC9038_00070) radC 71138..71806 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  EL018_RS00340 (NCTC9038_00071) rpmB 72023..72259 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  EL018_RS00345 (NCTC9038_00072) rpmG 72280..72447 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  EL018_RS00350 (NCTC9038_00073) mutM 72545..73354 (+) 810 WP_001114529.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EL018_RS00355 (NCTC9038_00074) coaD 73393..73872 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  EL018_RS00360 (NCTC9038_00075) waaA 73880..75157 (-) 1278 WP_000891566.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EL018_RS00365 (NCTC9038_00077) rfaQ 75564..76634 (+) 1071 WP_001311221.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=997007 EL018_RS00335 WP_001298959.1 71138..71806(+) (radC) [Escherichia coli strain NCTC9038]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=997007 EL018_RS00335 WP_001298959.1 71138..71806(+) (radC) [Escherichia coli strain NCTC9038]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGTTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment