Detailed information NTG0996271

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLNCDO184_RS07895
• Genome accession: NZ_CP155124
• Coordinates: 1515820..1516467 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain NCDO184
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1510820..1521467

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLNCDO184_RS07870 (LLNCDO184_07855)	atpF	1510891..1511397 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LLNCDO184_RS07875 (LLNCDO184_07860)	atpB	1511412..1512125 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLNCDO184_RS07880 (LLNCDO184_07865)	-	1512170..1512385 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLNCDO184_RS07885 (LLNCDO184_07870)	-	1512569..1513345 (-)	777	WP_063283279.1	alpha/beta hydrolase family protein	-
LLNCDO184_RS07890 (LLNCDO184_07875)	-	1513630..1515839 (-)	2210	Protein_1536	DNA internalization-related competence protein ComEC/Rec2	-
LLNCDO184_RS07895 (LLNCDO184_07880)	comEA	1515820..1516467 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
LLNCDO184_RS07900 (LLNCDO184_07885)	-	1516527..1517888 (-)	1362	WP_021214597.1	ABC transporter permease	-
LLNCDO184_RS07905 (LLNCDO184_07890)	-	1517885..1518817 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLNCDO184_RS07910 (LLNCDO184_07895)	-	1518922..1519320 (-)	399	WP_004255236.1	hypothetical protein	-
LLNCDO184_RS07915 (LLNCDO184_07900)	-	1519424..1519987 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLNCDO184_RS07920 (LLNCDO184_07905)	-	1520160..1521338 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=996271 LLNCDO184_RS07895 WP_010906131.1 1515820..1516467(-) (comEA) [Lactococcus lactis subsp. lactis strain NCDO184]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=996271 LLNCDO184_RS07895 WP_010906131.1 1515820..1516467(-) (comEA) [Lactococcus lactis subsp. lactis strain NCDO184]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386