Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M9Y83_RS27175 Genome accession   NZ_CP155048
Coordinates   5716078..5717778 (+) Length   566 a.a.
NCBI ID   WP_023835879.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CCBH26428     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5696395..5715225 5716078..5717778 flank 853


Gene organization within MGE regions


Location: 5696395..5717778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9Y83_RS27035 (M9Y83_0027035) - 5696395..5697111 (-) 717 WP_073624880.1 hypothetical protein -
  M9Y83_RS27040 (M9Y83_0027040) - 5697260..5697460 (+) 201 WP_023435053.1 helix-turn-helix domain-containing protein -
  M9Y83_RS27045 (M9Y83_0027045) - 5697457..5697873 (+) 417 WP_023435054.1 hypothetical protein -
  M9Y83_RS27050 (M9Y83_0027050) - 5697866..5698099 (+) 234 WP_023110507.1 hypothetical protein -
  M9Y83_RS27055 (M9Y83_0027055) - 5698096..5698344 (+) 249 WP_023435055.1 hypothetical protein -
  M9Y83_RS27060 (M9Y83_0027060) - 5698341..5698646 (+) 306 WP_023109345.1 hypothetical protein -
  M9Y83_RS27065 (M9Y83_0027065) - 5698649..5699086 (+) 438 WP_305954514.1 hypothetical protein -
  M9Y83_RS27070 (M9Y83_0027070) - 5699125..5699592 (+) 468 WP_031285502.1 hypothetical protein -
  M9Y83_RS27075 (M9Y83_0027075) - 5699599..5700552 (+) 954 WP_312024660.1 DUF3631 domain-containing protein -
  M9Y83_RS27080 (M9Y83_0027080) - 5700552..5700845 (+) 294 WP_023435057.1 hypothetical protein -
  M9Y83_RS27085 (M9Y83_0027085) - 5700901..5701803 (+) 903 WP_023435058.1 hypothetical protein -
  M9Y83_RS27090 (M9Y83_0027090) - 5702727..5702891 (+) 165 WP_153519866.1 hypothetical protein -
  M9Y83_RS27095 (M9Y83_0027095) - 5702908..5704500 (+) 1593 WP_079390841.1 anti-phage dCTP deaminase -
  M9Y83_RS27100 (M9Y83_0027100) - 5705118..5705624 (-) 507 WP_033982511.1 GNAT family N-acetyltransferase -
  M9Y83_RS27105 (M9Y83_0027105) - 5706385..5706666 (+) 282 WP_033978094.1 hypothetical protein -
  M9Y83_RS27110 (M9Y83_0027110) - 5706666..5706902 (+) 237 WP_003096116.1 hypothetical protein -
  M9Y83_RS27115 (M9Y83_0027115) - 5707109..5707390 (-) 282 WP_023435065.1 hypothetical protein -
  M9Y83_RS27120 (M9Y83_0027120) - 5707587..5708285 (+) 699 WP_124014670.1 hypothetical protein -
  M9Y83_RS27125 (M9Y83_0027125) - 5708310..5709314 (+) 1005 WP_031689993.1 pesticin C-terminus-like muramidase -
  M9Y83_RS27130 (M9Y83_0027130) - 5709327..5709989 (+) 663 WP_033992423.1 DUF3218 family protein -
  M9Y83_RS27135 (M9Y83_0027135) fliP 5710269..5711024 (+) 756 WP_128662130.1 flagellar type III secretion system pore protein FliP -
  M9Y83_RS27140 (M9Y83_0027140) fliQ 5711069..5711338 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  M9Y83_RS27145 (M9Y83_0027145) fliR 5711338..5712120 (+) 783 Protein_5374 flagellar biosynthetic protein FliR -
  M9Y83_RS27150 (M9Y83_0027150) - 5712123..5712382 (+) 260 Protein_5375 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  M9Y83_RS27155 (M9Y83_0027155) - 5713411..5713653 (+) 243 WP_023082027.1 hypothetical protein -
  M9Y83_RS27165 (M9Y83_0027165) - 5713912..5715225 (-) 1314 WP_234426410.1 O-antigen ligase family protein -
  M9Y83_RS27170 (M9Y83_0027170) - 5715383..5715847 (-) 465 WP_250180515.1 pilin -
  M9Y83_RS27175 (M9Y83_0027175) pilB 5716078..5717778 (+) 1701 WP_023835879.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62308.22 Da        Isoelectric Point: 5.4470

>NTDB_id=995800 M9Y83_RS27175 WP_023835879.1 5716078..5717778(+) (pilB) [Pseudomonas aeruginosa strain CCBH26428]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAEPLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=995800 M9Y83_RS27175 WP_023835879.1 5716078..5717778(+) (pilB) [Pseudomonas aeruginosa strain CCBH26428]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGC
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAAGCCCTGCTGGTAGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTTTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGACGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTATCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTATCGAAGAACAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GCCCCTCACCCGCCTGCTGAACATGGGTGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.368

100

0.578
  pilB Acinetobacter baylyi ADP1

56.614

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.321

100

0.544
  pilB Vibrio cholerae strain A1552

51.342

98.763

0.507
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

51.97

94.17

0.489
  pilB Vibrio campbellii strain DS40M4

51.032

94.17

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.664

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.727

97.173

0.376

Multiple sequence alignment