Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   AAVC94_RS16470 Genome accession   NZ_CP154906
Coordinates   3178263..3179387 (-) Length   374 a.a.
NCBI ID   WP_253622114.1    Uniprot ID   -
Organism   Bacillus pumilus strain FUA2115     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3173263..3184387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVC94_RS16430 (AAVC94_16430) flgL 3173443..3174354 (-) 912 WP_137054345.1 flagellar hook-associated protein FlgL -
  AAVC94_RS16435 (AAVC94_16435) flgK 3174364..3175884 (-) 1521 WP_253622115.1 flagellar hook-associated protein FlgK -
  AAVC94_RS16440 (AAVC94_16440) - 3175900..3176382 (-) 483 WP_050944342.1 flagellar protein FlgN -
  AAVC94_RS16445 (AAVC94_16445) flgM 3176401..3176664 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  AAVC94_RS16450 (AAVC94_16450) - 3176744..3177163 (-) 420 WP_003212865.1 TIGR03826 family flagellar region protein -
  AAVC94_RS16455 (AAVC94_16455) comFC 3177220..3177726 (-) 507 WP_050782676.1 phosphoribosyltransferase family protein Machinery gene
  AAVC94_RS16460 (AAVC94_16460) - 3177739..3177906 (-) 168 Protein_3174 ComF family protein -
  AAVC94_RS16465 (AAVC94_16465) - 3177903..3178196 (-) 294 WP_106066158.1 late competence development ComFB family protein -
  AAVC94_RS16470 (AAVC94_16470) comFA 3178263..3179387 (-) 1125 WP_253622114.1 DEAD/DEAH box helicase Machinery gene
  AAVC94_RS16475 (AAVC94_16475) - 3179762..3180604 (-) 843 WP_226804196.1 DegV family protein -
  AAVC94_RS16480 (AAVC94_16480) degU 3180822..3181511 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  AAVC94_RS16485 (AAVC94_16485) degS 3181576..3182751 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  AAVC94_RS16490 (AAVC94_16490) - 3182973..3183641 (+) 669 WP_003212936.1 YigZ family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 42753.12 Da        Isoelectric Point: 10.4626

>NTDB_id=995089 AAVC94_RS16470 WP_253622114.1 3178263..3179387(-) (comFA) [Bacillus pumilus strain FUA2115]
MGKTTECGFLYEWTGPQMEEACHSELTWQGNLSKGQKRASEKLIEAIKNKSDLLIWAVCGAGKTEVLFHGIEYALHQGMS
VCIATPRTDVVLELEPRLKKAFQGMKIAVLYGGSPQRFQIAPLVIATTHQLMRYKHAFDVLIIDEVDAFPYSIDERLQFA
VLKAMRKKGVRVYLSATPSKKMIRDVSRGQLEAIKIPLRFHQQPLPVPSFQWIGHWKKKLKKNQLPPKVMNWMQKHITKK
RRVLLFVPSISTMKKVTKVLRKHHLNVEGVSADDPDRKQKVQQFRDYKYDVLVTTTILERGVTISNVQVGVLGAESTIFT
ESALVQISGRAGRHLDFFKGDVFFFHFGLTRSMKQAMKHIVKMNDTAEKEFSEK

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=995089 AAVC94_RS16470 WP_253622114.1 3178263..3179387(-) (comFA) [Bacillus pumilus strain FUA2115]
ATGGGGAAAACAACGGAATGTGGCTTTCTTTATGAATGGACGGGTCCACAAATGGAAGAAGCATGTCACTCGGAATTAAC
ATGGCAGGGAAACCTGTCTAAAGGACAAAAAAGAGCGTCAGAGAAATTGATCGAAGCGATAAAAAACAAATCTGATCTAC
TCATCTGGGCAGTTTGCGGGGCAGGGAAAACAGAGGTGCTTTTTCACGGAATAGAATATGCCTTACATCAAGGAATGAGT
GTCTGCATTGCGACACCTAGAACTGATGTTGTCCTTGAACTAGAGCCGCGGCTTAAAAAAGCTTTTCAAGGAATGAAAAT
AGCTGTTCTTTATGGAGGAAGTCCTCAAAGGTTCCAAATAGCACCGTTAGTAATCGCTACAACTCACCAGTTGATGAGAT
ATAAACATGCATTTGATGTGCTCATTATAGATGAAGTCGACGCCTTCCCTTATTCAATCGATGAACGTCTCCAATTTGCT
GTTTTAAAGGCAATGAGAAAGAAAGGGGTTAGGGTTTATTTAAGTGCGACGCCGTCTAAGAAAATGATAAGAGATGTATC
TCGTGGACAACTAGAAGCCATAAAAATTCCTCTGCGCTTTCACCAACAACCTTTACCGGTCCCATCCTTTCAATGGATTG
GACATTGGAAGAAGAAATTAAAAAAGAATCAGCTCCCACCTAAAGTAATGAATTGGATGCAGAAACATATTACAAAAAAG
AGAAGGGTTTTACTTTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACGAAGGTTCTTAGAAAGCACCATTTAAATGT
AGAAGGGGTGTCTGCTGATGATCCAGATAGGAAACAAAAGGTCCAGCAATTCAGAGATTACAAATACGATGTACTAGTTA
CAACCACAATTCTAGAAAGAGGTGTAACCATTTCAAATGTGCAAGTGGGGGTACTAGGTGCAGAATCTACTATCTTTACA
GAAAGTGCACTTGTTCAGATTTCTGGTAGAGCAGGTAGACATCTCGATTTTTTTAAAGGAGATGTTTTCTTTTTTCATTT
TGGTTTAACGAGAAGTATGAAACAAGCAATGAAGCATATTGTAAAAATGAATGATACAGCTGAAAAAGAGTTCTCCGAAA
AATAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(31-179)

Helicase conserved C-terminal domain (Helicase_C)

(235-333)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

58.266

98.663

0.575
  comFA Latilactobacillus sakei subsp. sakei 23K

39.459

98.93

0.39
  comFA/cflA Streptococcus mitis SK321

40.678

94.652

0.385
  comFA/cflA Streptococcus pneumoniae Rx1

40.113

94.652

0.38
  comFA/cflA Streptococcus pneumoniae D39

40.113

94.652

0.38
  comFA/cflA Streptococcus pneumoniae R6

40.113

94.652

0.38
  comFA/cflA Streptococcus pneumoniae TIGR4

39.831

94.652

0.377
  comFA/cflA Streptococcus mitis NCTC 12261

39.548

94.652

0.374
  comFA Lactococcus lactis subsp. cremoris KW2

38.162

95.989

0.366

Multiple sequence alignment