Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   AAIS09_RS09570 Genome accession   NZ_CP154595
Coordinates   1871753..1872769 (-) Length   338 a.a.
NCBI ID   WP_075270915.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain serotype 4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1866753..1877769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIS09_RS09525 (AAIS09_09510) - 1866837..1866968 (-) 132 WP_000768904.1 hypothetical protein -
  AAIS09_RS09530 (AAIS09_09515) - 1866969..1868159 (-) 1191 WP_000167766.1 acetate kinase -
  AAIS09_RS09535 (AAIS09_09520) comYH 1868210..1869163 (-) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  AAIS09_RS09540 (AAIS09_09525) - 1869224..1869811 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  AAIS09_RS09545 (AAIS09_09530) comGG/cglG 1869948..1870361 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  AAIS09_RS09550 (AAIS09_09535) comGF/cglF 1870339..1870800 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  AAIS09_RS09555 (AAIS09_09540) comGE/cglE 1870763..1871065 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  AAIS09_RS09560 (AAIS09_09545) comGD/cglD 1871028..1871432 (-) 405 WP_000588006.1 competence type IV pilus minor pilin ComGD Machinery gene
  AAIS09_RS09565 (AAIS09_09550) comGC/cglC 1871425..1871751 (-) 327 WP_000738638.1 comG operon protein ComGC Machinery gene
  AAIS09_RS09570 (AAIS09_09555) comGB/cglB 1871753..1872769 (-) 1017 WP_075270915.1 competence type IV pilus assembly protein ComGB Machinery gene
  AAIS09_RS09575 (AAIS09_09560) comGA/cglA/cilD 1872717..1873658 (-) 942 WP_000249550.1 competence type IV pilus ATPase ComGA Machinery gene
  AAIS09_RS09580 (AAIS09_09565) - 1873734..1874099 (-) 366 WP_000286415.1 DUF1033 family protein -
  AAIS09_RS09585 (AAIS09_09570) - 1874250..1875308 (-) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -
  AAIS09_RS09590 (AAIS09_09575) nagA 1875471..1876622 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38496.55 Da        Isoelectric Point: 9.6162

>NTDB_id=993718 AAIS09_RS09570 WP_075270915.1 1871753..1872769(-) (comGB/cglB) [Streptococcus pneumoniae strain serotype 4]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRAGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=993718 AAIS09_RS09570 WP_075270915.1 1871753..1872769(-) (comGB/cglB) [Streptococcus pneumoniae strain serotype 4]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGCGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTCCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAAATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae D39

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae R6

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae TIGR4

98.817

100

0.988
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment