Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   AAIS09_RS09065 Genome accession   NZ_CP154595
Coordinates   1791204..1792028 (-) Length   274 a.a.
NCBI ID   WP_050204516.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain serotype 4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1786204..1797028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIS09_RS09060 (AAIS09_09045) - 1789644..1790975 (-) 1332 WP_000390049.1 hemolysin family protein -
  AAIS09_RS09065 (AAIS09_09050) endA 1791204..1792028 (-) 825 WP_050204516.1 DNA-entry nuclease EndA Machinery gene
  AAIS09_RS09070 (AAIS09_09055) - 1792067..1792255 (-) 189 WP_001818955.1 DNA-directed RNA polymerase subunit beta -
  AAIS09_RS09075 (AAIS09_09060) murA 1792248..1793531 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  AAIS09_RS09080 (AAIS09_09065) - 1793555..1793785 (-) 231 WP_000250393.1 DUF1146 family protein -
  AAIS09_RS09085 (AAIS09_09070) sepM 1793843..1794880 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  AAIS09_RS09090 (AAIS09_09075) coaD 1794864..1795352 (-) 489 WP_001280740.1 pantetheine-phosphate adenylyltransferase -
  AAIS09_RS09095 (AAIS09_09080) rsmD 1795342..1795881 (-) 540 WP_369916964.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  AAIS09_RS09100 (AAIS09_09085) asnA 1795946..1796938 (-) 993 WP_000747995.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29969.67 Da        Isoelectric Point: 10.1700

>NTDB_id=993707 AAIS09_RS09065 WP_050204516.1 1791204..1792028(-) (endA) [Streptococcus pneumoniae strain serotype 4]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKTTRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=993707 AAIS09_RS09065 WP_050204516.1 1791204..1792028(-) (endA) [Streptococcus pneumoniae strain serotype 4]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCTAAAACCAAGCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGACCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCTAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.175

100

0.982
  endA Streptococcus pneumoniae D39

98.175

100

0.982
  endA Streptococcus pneumoniae R6

98.175

100

0.982
  endA Streptococcus pneumoniae TIGR4

98.175

100

0.982

Multiple sequence alignment