Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAHN93_RS13525 Genome accession   NZ_CP154474
Coordinates   3043720..3045450 (+) Length   576 a.a.
NCBI ID   WP_343292826.1    Uniprot ID   -
Organism   Vandammella animalimorsus strain W29-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3038720..3050450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHN93_RS13505 (AAHN93_13505) corA 3039670..3040653 (-) 984 WP_095550010.1 magnesium/cobalt transporter CorA -
  AAHN93_RS13510 (AAHN93_13510) - 3040913..3041395 (-) 483 WP_343292824.1 uracil-DNA glycosylase family protein -
  AAHN93_RS13515 (AAHN93_13515) rimI 3041946..3042446 (-) 501 WP_343292825.1 ribosomal protein S18-alanine N-acetyltransferase -
  AAHN93_RS13520 (AAHN93_13520) - 3042470..3043387 (-) 918 WP_095551476.1 peptidoglycan DD-metalloendopeptidase family protein -
  AAHN93_RS13525 (AAHN93_13525) pilB 3043720..3045450 (+) 1731 WP_343292826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAHN93_RS13530 (AAHN93_13530) pilC 3045481..3046722 (+) 1242 WP_095550006.1 type II secretion system F family protein Machinery gene
  AAHN93_RS13535 (AAHN93_13535) - 3046722..3047603 (+) 882 WP_343292827.1 prepilin peptidase -
  AAHN93_RS13540 (AAHN93_13540) - 3047820..3048812 (-) 993 WP_343292828.1 tripartite tricarboxylate transporter substrate binding protein -
  AAHN93_RS13545 (AAHN93_13545) - 3048923..3050149 (-) 1227 WP_343292829.1 CaiB/BaiF CoA-transferase family protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62972.93 Da        Isoelectric Point: 4.8096

>NTDB_id=993564 AAHN93_RS13525 WP_343292826.1 3043720..3045450(+) (pilB) [Vandammella animalimorsus strain W29-2]
MATEDLTKKATAELVVPSVARAFLVAKQLTQEQVDATLQRARNEGRDFLSALVEGNVLSATQAAKTISDVFAVPYIDLGS
VDAMRLPADLLDLKLCLSYKVVVLGKRQNRLIVASADPSNRDTAEKLKFATQASIEWLVAAHDKIAALLDELAKANALDG
QSSDFQFDELDGAVEDVTEESASDPSSDAEDAPVVRFLQKMIIDAYRLGASDLHFEPYEFNYRVRFRIDGELREYAAPPV
SIKERLASRIKVISRLDIAEKRIPQDGRTKMRIGGKSIDFRVSTLPTLFGEKIVIRILDSSNAKIGIDALGYEPIEKERL
LTAIERPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINLPGVNQVNVNEKAGLTFAAALKSFLRQDPDI
IMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRLRNMGIAPFNIASSVILITAQRLARRLCNQCKEETDIPVD
ALIDAGFNYDDLDGSWVPYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILNDGSAIEIQEQAEKEGVRTLRQSGLQKV
KLGVTSLEEVLAVTNE

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=993564 AAHN93_RS13525 WP_343292826.1 3043720..3045450(+) (pilB) [Vandammella animalimorsus strain W29-2]
ATGGCCACTGAAGATCTCACCAAAAAAGCCACTGCCGAACTGGTTGTGCCCAGCGTTGCCAGGGCATTTCTGGTTGCCAA
GCAGCTGACGCAGGAGCAGGTGGATGCCACGCTGCAGCGGGCTCGCAACGAAGGGCGGGACTTTCTCAGTGCATTGGTCG
AAGGCAATGTCCTGTCCGCTACACAGGCGGCCAAAACCATTTCGGATGTTTTTGCCGTGCCCTACATCGACCTGGGATCT
GTCGATGCCATGCGTTTGCCAGCCGATCTGTTGGATTTGAAGCTATGCCTGTCCTACAAAGTGGTTGTGCTGGGCAAGCG
CCAGAACCGGCTGATCGTCGCCAGCGCCGACCCCAGCAATCGCGATACGGCGGAGAAGCTGAAGTTTGCCACCCAGGCCT
CCATCGAGTGGCTGGTGGCGGCGCACGACAAGATCGCAGCCTTGCTCGATGAGCTGGCCAAGGCCAATGCACTTGATGGC
CAGTCTTCGGACTTCCAGTTCGACGAGCTCGATGGTGCGGTGGAGGACGTGACAGAAGAAAGCGCCAGCGATCCTAGCAG
TGATGCCGAGGATGCGCCGGTGGTACGGTTTTTGCAGAAAATGATCATTGATGCCTACCGTCTGGGGGCTTCAGACTTGC
ATTTCGAGCCGTATGAATTCAATTACCGTGTGCGTTTTCGCATTGACGGTGAGTTGCGTGAATATGCTGCACCGCCTGTT
TCCATCAAGGAGCGCCTGGCATCTCGAATTAAAGTGATCTCCCGATTGGATATCGCCGAGAAACGCATCCCGCAGGATGG
TCGCACCAAAATGCGCATTGGCGGAAAATCCATCGATTTTCGTGTCAGTACCTTGCCTACATTGTTTGGCGAGAAAATCG
TCATTCGTATTCTGGATTCCAGCAACGCCAAAATCGGCATTGATGCGCTGGGCTACGAGCCGATTGAGAAGGAGCGTCTG
CTCACGGCCATTGAGCGTCCATATGGCATGGTGCTGGTGACCGGGCCAACGGGCTCGGGTAAAACGGTGTCCTTGTACAC
CTGTCTGAACCTGTTGAACAAACCAGGGGTGAATATTTCCACGGCTGAGGACCCCTCGGAAATCAACCTGCCGGGCGTGA
ATCAGGTCAACGTCAATGAAAAGGCTGGCTTGACGTTTGCTGCAGCCCTGAAATCGTTTTTGCGCCAGGATCCCGACATC
ATCATGGTGGGTGAGATCCGTGACCTGGAAACGGCGGACATCGCCATCAAGGCCGCCCAAACCGGGCACTTGGTCTTGTC
CACGCTGCACACCAACGATGCCCCGACCACATTGACTCGATTGCGCAATATGGGGATTGCTCCCTTCAATATCGCTTCCA
GCGTGATTCTGATCACTGCGCAGCGCTTGGCGCGTCGCCTGTGCAATCAGTGCAAGGAAGAAACCGATATTCCCGTGGAT
GCCTTGATCGATGCCGGGTTCAATTACGACGATCTCGATGGCTCCTGGGTGCCTTATAAACCAGTGGGCTGCTCGGCGTG
CAACAATGGCTACAAGGGCCGGGTGGGCATTTATCAGGTGATGCCCATCAGTGAGGAAATCCAACGCATTATTCTCAATG
ATGGCTCTGCCATCGAAATTCAGGAGCAGGCTGAAAAAGAAGGCGTGCGCACACTGCGCCAGTCTGGCTTGCAAAAAGTC
AAGCTGGGCGTGACTTCTTTGGAAGAAGTGCTGGCTGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.464

97.222

0.53
  pilF Neisseria gonorrhoeae MS11

54.301

96.875

0.526
  pilB Acinetobacter baylyi ADP1

53.176

95.66

0.509
  pilB Legionella pneumophila strain ERS1305867

50.697

99.653

0.505
  pilB Vibrio cholerae strain A1552

47.715

94.965

0.453
  pilB Vibrio campbellii strain DS40M4

46.084

95.313

0.439
  pilB Vibrio parahaemolyticus RIMD 2210633

45.238

94.792

0.429
  pilF Thermus thermophilus HB27

39.928

96.528

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

95.486

0.372

Multiple sequence alignment