Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AAIB12_RS06145 Genome accession   NZ_CP154469
Coordinates   1307579..1308013 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain Pm3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1302579..1313013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIB12_RS06115 (AAIB12_06115) - 1303143..1303412 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  AAIB12_RS06120 (AAIB12_06120) yacG 1303412..1303615 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  AAIB12_RS06125 (AAIB12_06125) coaE 1303626..1304246 (-) 621 WP_010906475.1 dephospho-CoA kinase -
  AAIB12_RS06130 (AAIB12_06130) - 1304261..1304983 (-) 723 WP_010906474.1 A24 family peptidase -
  AAIB12_RS06135 (AAIB12_06135) - 1304983..1306194 (-) 1212 WP_005722560.1 type II secretion system F family protein -
  AAIB12_RS06140 (AAIB12_06140) pilB 1306194..1307579 (-) 1386 WP_010906472.1 GspE/PulE family protein Machinery gene
  AAIB12_RS06145 (AAIB12_06145) pilA 1307579..1308013 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  AAIB12_RS06150 (AAIB12_06150) ampD 1308173..1308739 (+) 567 WP_005751074.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AAIB12_RS06155 (AAIB12_06155) rppH 1309324..1309920 (+) 597 WP_343298151.1 RNA pyrophosphohydrolase -
  AAIB12_RS06160 (AAIB12_06160) - 1309923..1310717 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  AAIB12_RS06165 (AAIB12_06165) lgt 1310727..1311539 (+) 813 WP_010906471.1 prolipoprotein diacylglyceryl transferase -
  AAIB12_RS06170 (AAIB12_06170) - 1311536..1312387 (+) 852 WP_010906470.1 thymidylate synthase -
  AAIB12_RS06175 (AAIB12_06175) tadA 1312402..1312902 (+) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=993513 AAIB12_RS06145 WP_005741096.1 1307579..1308013(-) (pilA) [Pasteurella multocida strain Pm3]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=993513 AAIB12_RS06145 WP_005741096.1 1307579..1308013(-) (pilA) [Pasteurella multocida strain Pm3]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCTGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACAGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment