Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAGD16_RS13235 Genome accession   NZ_CP152390
Coordinates   2876116..2877792 (+) Length   558 a.a.
NCBI ID   WP_024601729.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. M58     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2871116..2882792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGD16_RS13210 nadC 2871118..2871963 (+) 846 WP_377112299.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AAGD16_RS13215 - 2872475..2872933 (+) 459 WP_377112302.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AAGD16_RS13220 pilC 2872998..2874227 (+) 1230 WP_377112305.1 type II secretion system F family protein Machinery gene
  AAGD16_RS13225 pilD 2874438..2875349 (+) 912 WP_377112308.1 prepilin peptidase Machinery gene
  AAGD16_RS13230 coaE 2875352..2875981 (+) 630 WP_377112311.1 dephospho-CoA kinase -
  AAGD16_RS13235 pilB 2876116..2877792 (+) 1677 WP_024601729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAGD16_RS13240 yacG 2877857..2878087 (+) 231 WP_008114915.1 DNA gyrase inhibitor YacG -
  AAGD16_RS13245 - 2878147..2878635 (-) 489 WP_377112315.1 Spy/CpxP family protein refolding chaperone -
  AAGD16_RS13250 - 2878803..2879492 (+) 690 WP_016899750.1 response regulator transcription factor -
  AAGD16_RS13255 - 2879470..2880897 (+) 1428 WP_377112319.1 ATP-binding protein -
  AAGD16_RS13260 - 2880887..2881831 (+) 945 WP_008114910.1 alpha/beta fold hydrolase -
  AAGD16_RS13265 - 2881828..2882640 (-) 813 WP_013465942.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62402.69 Da        Isoelectric Point: 6.1023

>NTDB_id=988878 AAGD16_RS13235 WP_024601729.1 2876116..2877792(+) (pilB) [Pseudoalteromonas sp. M58]
MNINSPLLRKFITLGRVDTDTVKTRQQDFASTAELISKCGKIDSKDLAEQCIDLFRVPFFDLKDFNVAQISAELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYVQLDNKIEQLLDATGSLNLSEDEFKEFAELDSEQ
HKEHTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCSKCKTPEQLPREELIRQGFSEQQIQDMVL
YAPKGCENCTDGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGYNNLRLSGIRKAAEGVTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=988878 AAGD16_RS13235 WP_024601729.1 2876116..2877792(+) (pilB) [Pseudoalteromonas sp. M58]
ATGAATATTAACTCACCGTTATTAAGAAAATTTATTACCCTCGGCCGGGTTGATACTGATACCGTAAAAACACGGCAGCA
AGATTTTGCGTCAACTGCAGAGCTTATCTCTAAATGTGGAAAAATAGACAGTAAAGATTTAGCTGAGCAATGCATTGATT
TATTTAGAGTGCCTTTTTTTGATTTAAAAGATTTTAATGTCGCACAAATATCTGCTGAGCTGGTTAAAGAAAAACTGATC
CGCAAACACCACATTTTGCCTTTGGTACAAAAAGGCAGAAAAGTGTATATTGCCGCCTCAGATCCTACCGATTATGGCGC
CTTCGAAAACTTTGAGTTTAGCACGGGTTTATCGTGCGAAGTGGTTGTGGTTGACTACGTCCAGCTCGATAATAAAATTG
AGCAATTACTCGATGCTACTGGCAGTTTAAATTTAAGTGAAGATGAATTTAAAGAGTTCGCTGAACTCGATTCTGAGCAG
CACAAAGAACACACTCCCAAAGACGATGACAAAGACGACGCCCCAATAATTGTCTACATTAATAAAATTTTAATGGATGC
AATAAAAAAAGGCGCCTCAGATTTACACTTTGAACCTTATGAACACAAATACCGTATTCGCTTTCGCATTGATGGTATTT
TGCACGAAGTAGCCAGCCCGCCAAATACTTTAGCCAGTAAATTATCAGCGCGAATAAAAGTAATGTCGCGACTGGATATT
GCAGAAAAGCGTAAACCACAAGATGGCCGTATTAAGCTTAAAATCACTGAACGTAAAAGTATTGATTTTCGTGTTAGTAC
CATGCCAACTTTGTGGGGCGAAAAAATTGTAATGCGTATTCTTGATTCATCCAGTGCCATGCTAGGTATTGATGTATTAG
GCTATGAACCCGAGCAGAAAAAACTATACCTCGATGCACTTGAGCAACCGCAAGGGATGATTTTGGTTACCGGCCCAACC
GGTTCAGGTAAAACGGTTTCTTTGTATACGGGCTTAAATATACTCAATCAGCCGGAGCGGAATATTAGTACCGCCGAAGA
TCCGGTAGAAATTAACCTCGAAGGGGTTAACCAAGTACAAATAAATACTAAAGCAGATATGACTTTTGCTAATGCACTGC
GGGCGTTTTTACGTCAAGATCCCGATGTAGTGATGGTGGGTGAAATACGTGATTTAGAAACTGCAGAAATATCAATTAAA
GCGGCGCAAACAGGTCACTTGGTGCTGTCAACACTGCATACCAACTCAGCGCCAGAAACGATTACTCGTTTGTTAAATAT
GGGTGTACCCGCGTATAATGTGGCCAGTTCTATTAGTTTAATTATAGCTCAGCGTTTAGCCCGAAGATTATGCTCTAAAT
GTAAAACACCAGAGCAGTTACCTCGCGAAGAGCTTATACGCCAAGGCTTTAGTGAACAACAAATTCAAGACATGGTTTTA
TACGCACCTAAAGGCTGTGAAAACTGTACCGATGGCTACAAAGGCCGTGTTGGTATTTACGAAGTAATGAAAATTACGCC
TGAAATTGCCCAAATTATAATGCGCGGCGGTAACTCATTAGAAATTGCTGAGGTATCACTTAAAGCAGGATATAACAATT
TGCGTTTATCGGGTATTCGTAAAGCCGCAGAGGGTGTGACCTCACTTGCTGAGATTAATCGTGTCACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.593

96.774

0.557
  pilB Acinetobacter baylyi ADP1

58.416

90.502

0.529
  pilB Legionella pneumophila strain ERS1305867

55.273

91.756

0.507
  pilB Vibrio cholerae strain A1552

52.06

95.699

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

51.685

95.699

0.495
  pilB Vibrio campbellii strain DS40M4

51.124

95.699

0.489
  pilF Neisseria gonorrhoeae MS11

49.808

93.548

0.466
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.382

92.832

0.366

Multiple sequence alignment