Detailed information NTG0988772

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: AAGT77_RS12670
• Genome accession: NZ_CP152380
• Coordinates: 2726954..2728666 (+)
• Length: 570 a.a.
• NCBI ID: WP_342630807.1
• Uniprot ID: -
• Organism: Marinobacter alkaliphilus strain SBY-1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2712938..2726006	2726954..2728666	flank	948

Gene organization within MGE regions

Location: 2712938..2728666

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAGT77_RS12595 (AAGT77_12595)	-	2712938..2713264 (+)	327	WP_342630793.1	nucleotidyltransferase domain-containing protein	-
AAGT77_RS12600 (AAGT77_12600)	-	2713589..2714218 (-)	630	WP_342630794.1	DUF2931 family protein	-
AAGT77_RS12605 (AAGT77_12605)	-	2714208..2715950 (-)	1743	WP_342630795.1	type VI secretion system PAAR protein	-
AAGT77_RS12610 (AAGT77_12610)	-	2716347..2717105 (+)	759	WP_342630796.1	hypothetical protein	-
AAGT77_RS12615 (AAGT77_12615)	-	2717260..2717646 (+)	387	WP_342630797.1	glutaredoxin	-
AAGT77_RS12620 (AAGT77_12620)	-	2717663..2717944 (+)	282	WP_342630798.1	pyrimidine/purine nucleoside phosphorylase	-
AAGT77_RS12625 (AAGT77_12625)	-	2718009..2718401 (-)	393	WP_342630799.1	DUF1622 domain-containing protein	-
AAGT77_RS12630 (AAGT77_12630)	-	2718545..2719417 (+)	873	WP_342630800.1	decaprenyl-phosphate phosphoribosyltransferase	-
AAGT77_RS12635 (AAGT77_12635)	-	2719414..2720385 (+)	972	WP_404810642.1	YbhN family protein	-
AAGT77_RS12640 (AAGT77_12640)	-	2720382..2721683 (+)	1302	WP_342630801.1	FAD-binding oxidoreductase	-
AAGT77_RS12645 (AAGT77_12645)	-	2721680..2722435 (+)	756	WP_342630802.1	SDR family oxidoreductase	-
AAGT77_RS12650 (AAGT77_12650)	-	2722516..2723091 (+)	576	WP_342630803.1	HAD-IB family hydrolase	-
AAGT77_RS12655 (AAGT77_12655)	-	2723255..2723725 (+)	471	WP_342630804.1	pilin	-
AAGT77_RS12660 (AAGT77_12660)	-	2724099..2726006 (-)	1908	WP_342630805.1	hypothetical protein	-
AAGT77_RS12665 (AAGT77_12665)	-	2726078..2726701 (-)	624	WP_342630806.1	pilin	-
AAGT77_RS12670 (AAGT77_12670)	pilB	2726954..2728666 (+)	1713	WP_342630807.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 570 a.a.
• Molecular weight: 62677.79 Da
• Isoelectric Point: 5.0094

>NTDB_id=988772 AAGT77_RS12670 WP_342630807.1 2726954..2728666(+) (pilB) [Marinobacter alkaliphilus strain SBY-1]
MANSNRNVTLTGLARRFVDDGLLDEDTAKDAFLQASQNRIPLITYLTQNHLADSSKLAFSAAMEFGVSVLDLDSFLPDMM
PEKVVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLRAAIDKYLESHDTTMGDLDDA
DLEGVETEGGDQDDDGAVSATEVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKIYRVRYRTDGILKEISRPSIKLAPKI
SARVKIMAQLDISERRIPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKQLYMDALA
QPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNTKVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSCKQPADIPKDVLLKEG
FTQEQIDTGFTLYRPKGCDKCTNGYKGRVGIYEVVKITEELASMIMEEASSIKIAKQAQAEGFRNLRQSALLKVMEGVTS
LEEANRVTKD

Nucleotide

• Length: 1713 bp

>NTDB_id=988772 AAGT77_RS12670 WP_342630807.1 2726954..2728666(+) (pilB) [Marinobacter alkaliphilus strain SBY-1]
ATGGCGAACAGCAACCGAAACGTGACCCTGACGGGGCTGGCCCGTCGATTTGTGGATGACGGGCTTCTGGACGAAGATAC
CGCCAAAGATGCCTTTCTTCAGGCCTCCCAGAACCGCATCCCACTGATCACCTACCTTACCCAGAACCACCTTGCGGACA
GTTCCAAACTGGCGTTTTCTGCTGCCATGGAGTTCGGCGTGTCGGTGCTGGACCTGGATTCTTTCCTGCCGGACATGATG
CCGGAGAAGGTTGTTGATGAGAAACTGGTGAGGAAGCATAACGCCCTGCCTCTTTATAAACGAGGCAACCGGCTGTTCAT
CGCAGTATCAGACCCCACAAATATTCAGGCACTCGATGAAATCAAGTTCAACACCGGGCTTAGCACCGATGCGATTCTGG
TGGACGACGCCAAGCTGCGCGCGGCAATAGACAAGTACCTCGAATCCCATGACACTACGATGGGAGACCTGGACGACGCC
GATCTGGAGGGCGTTGAGACCGAGGGTGGTGACCAGGACGACGATGGTGCCGTCAGTGCTACCGAAGTAGATGACGCACC
TATTGTTAAGTATGTGAACAAGATGCTGCTCGACGCTATTCGTGGCGGCGCATCCGATATTCACTTTGAGCCCTACGAAA
AGATCTATCGGGTACGCTACCGGACCGATGGCATCCTGAAGGAAATTTCCCGCCCTTCTATCAAGCTCGCACCGAAGATC
TCTGCCCGGGTAAAGATCATGGCGCAACTGGACATCTCGGAGCGGCGTATTCCTCAGGACGGTCGGATCAAAATGAAGCT
GTCCAAGACCAAGGCCATCGACTTCCGGGTAAACACCCTCCCCACCCTCTGGGGCGAAAAAATTGTTCTGCGGATTCTGG
ACCCGAGCCAGGCCAAGCTGGGCATTGACGTGCTGGGTTACGAAGAAGATCAGAAGCAACTGTATATGGATGCACTGGCC
CAGCCCCAGGGTATGATTCTGGTAACCGGGCCGACCGGTTCCGGTAAGACGGTATCGCTCTACACCGGCCTGAACATTCT
GAACACTCCGGGCATCAACATTTCTACAGCGGAAGACCCGGCAGAGATTAACCTGGAAGGGATCAACCAGGTTAACGTGA
ATACCAAAGTGGGCCTCGGCTTCGCTGAAGCCCTTCGGGCCTTCCTGCGGCAAGACCCGGATGTGATCATGGTGGGTGAG
ATCCGGGATCTCGAAACCGCGAACATTGCGATCAAAGCGGCGCAAACCGGGCATTTGGTTCTGTCTACCCTGCACACCAA
CAGCGCGGCCGAAACCCTCACGCGAATGATGAACATGGGGGTGCCTGCGTTCAACATTGCCACGTCCGTGAGCCTGATTA
TTGCCCAGCGCCTTGGCCGGCGCCTGTGCAGCTGCAAGCAGCCGGCAGACATCCCGAAAGACGTCCTTTTGAAGGAAGGG
TTCACACAAGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCACTAATGGATATAAAGG
CCGCGTCGGTATTTACGAGGTGGTAAAGATTACCGAAGAACTGGCGAGCATGATTATGGAAGAAGCCAGTTCCATAAAAA
TTGCAAAACAAGCGCAGGCAGAGGGCTTCCGTAACCTGAGGCAATCCGCCCTTCTGAAAGTGATGGAAGGGGTGACCAGC
CTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (46-150)

• Type II/IV secretion system protein (T2SSE) (194-461)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	58.082	98.772	0.574
pilB	Acinetobacter baylyi ADP1	57.194	98.772	0.565
pilB	Legionella pneumophila strain ERS1305867	54.087	100	0.546
pilB	Vibrio parahaemolyticus RIMD 2210633	52.065	97.719	0.509
pilB	Vibrio campbellii strain DS40M4	51.064	98.947	0.505
pilB	Vibrio cholerae strain A1552	54.143	91.053	0.493
pilF	Neisseria gonorrhoeae MS11	48.41	99.298	0.481
pilB/pilB1	Synechocystis sp. PCC 6803	40.655	91.053	0.37
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.544	92.281	0.365
pilF	Thermus thermophilus HB27	37.365	97.193	0.363