Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NSQ52_RS16805 Genome accession   NZ_CP151973
Coordinates   3255332..3256459 (-) Length   375 a.a.
NCBI ID   WP_342473620.1    Uniprot ID   -
Organism   Bacillus sp. FSL W8-1141     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3250332..3261459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ52_RS16770 (NSQ52_16770) flgL 3250515..3251426 (-) 912 WP_039169789.1 flagellar hook-associated protein FlgL -
  NSQ52_RS16775 (NSQ52_16775) flgK 3251435..3252955 (-) 1521 WP_099496871.1 flagellar hook-associated protein FlgK -
  NSQ52_RS16780 (NSQ52_16780) - 3252971..3253453 (-) 483 WP_342473618.1 flagellar protein FlgN -
  NSQ52_RS16785 (NSQ52_16785) flgM 3253472..3253735 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  NSQ52_RS16790 (NSQ52_16790) - 3253815..3254234 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  NSQ52_RS16795 (NSQ52_16795) comFC 3254291..3254977 (-) 687 WP_172793290.1 ComF family protein Machinery gene
  NSQ52_RS16800 (NSQ52_16800) - 3254974..3255267 (-) 294 WP_008348267.1 competence protein ComFB -
  NSQ52_RS16805 (NSQ52_16805) comFA 3255332..3256459 (-) 1128 WP_342473620.1 DEAD/DEAH box helicase Machinery gene
  NSQ52_RS16810 (NSQ52_16810) - 3256831..3257673 (-) 843 WP_342473622.1 DegV family protein -
  NSQ52_RS16815 (NSQ52_16815) degU 3257885..3258574 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  NSQ52_RS16820 (NSQ52_16820) degS 3258639..3259814 (-) 1176 WP_024719243.1 sensor histidine kinase Regulator
  NSQ52_RS16825 (NSQ52_16825) - 3260036..3260704 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42879.87 Da        Isoelectric Point: 10.4727

>NTDB_id=982809 NSQ52_RS16805 WP_342473620.1 3255332..3256459(-) (comFA) [Bacillus sp. FSL W8-1141]
MLGKVSECGFLYEWTGPQKKETRQVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVARRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQGQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
SESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=982809 NSQ52_RS16805 WP_342473620.1 3255332..3256459(-) (comFA) [Bacillus sp. FSL W8-1141]
ATGCTGGGAAAAGTAAGCGAATGTGGCTTTCTCTACGAATGGACGGGTCCACAAAAGAAAGAAACAAGACAAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCATGGAATAAAATATGCGTTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTTGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCGCCGCTTATGATCGCTACAACCCATCAGCTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTAGACGAAGTCGATGCTTTTCCTTATTCAATGGATGAACGGCTTCAGTTT
GCTGTTTTAAAGTCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAAGATGT
TGCTCGTAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGGTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGATATGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAGGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
TCAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTATACTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.946

98.667

0.552
  comFA/cflA Streptococcus pneumoniae Rx1

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae D39

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae R6

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae TIGR4

40.678

94.4

0.384
  comFA/cflA Streptococcus mitis NCTC 12261

40.395

94.4

0.381
  comFA Latilactobacillus sakei subsp. sakei 23K

38.587

98.133

0.379
  comFA/cflA Streptococcus mitis SK321

39.266

94.4

0.371
  comFA Lactococcus lactis subsp. cremoris KW2

37.432

97.6

0.365

Multiple sequence alignment