Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAFF32_RS07810 Genome accession   NZ_CP151802
Coordinates   1694511..1696232 (-) Length   573 a.a.
NCBI ID   WP_342316996.1    Uniprot ID   -
Organism   Lysobacter sp. FW306-1B-D06B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1689511..1701232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFF32_RS07790 (AAFF32_07790) - 1690641..1691639 (+) 999 WP_342316994.1 Nudix family hydrolase -
  AAFF32_RS07795 (AAFF32_07795) coaE 1691666..1692283 (-) 618 WP_342316995.1 dephospho-CoA kinase -
  AAFF32_RS07800 (AAFF32_07800) - 1692339..1693202 (-) 864 WP_216960201.1 A24 family peptidase -
  AAFF32_RS07805 (AAFF32_07805) pilC 1693248..1694507 (-) 1260 WP_216960198.1 type II secretion system F family protein Machinery gene
  AAFF32_RS07810 (AAFF32_07810) pilB 1694511..1696232 (-) 1722 WP_342316996.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAFF32_RS07815 (AAFF32_07815) - 1696330..1699371 (-) 3042 WP_342316997.1 fused MFS/spermidine synthase -
  AAFF32_RS07820 (AAFF32_07820) - 1699473..1699886 (-) 414 WP_342316998.1 pilin -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62419.71 Da        Isoelectric Point: 6.3338

>NTDB_id=980930 AAFF32_RS07810 WP_342316996.1 1694511..1696232(-) (pilB) [Lysobacter sp. FW306-1B-D06B]
MNVAATANLVGITGIARRLVMDGALDDNAARQALAAATAAKQPITAYLAEKRLVTPGQLAAANSIEFGVPLFDATVLDAS
QSAIKLVSESLVRKHQILPLYKRGNRLFVGVSDPTNTLALDEIKFQSNLAIEPILISEDQIQRCIEKWLEVSDNLADTVG
NDEGLENLDISGGDDIAADNTVDVKTDDTPVVKFVNKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQVTKVPTNLS
GRIAARLKVMSQLDIAEKRVPQDGRIKLNISKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEDDQRALYEQ
ALAKPYGMVLVTGPTGSGKTVSLYTGLNILNEEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDVVM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTVSRLMNMGIAPYNITSSVTLIIAQRLARRLHDCKQEVHLPEHALL
AEGFTHKEVEAGIKIYEAVGCSDCTEGYKGRTGIYQVMPMTEKIQAIILQGGNALDIAEAAKQSGVRDLRMSALLKVKNG
VTSLAEINRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=980930 AAFF32_RS07810 WP_342316996.1 1694511..1696232(-) (pilB) [Lysobacter sp. FW306-1B-D06B]
ATGAACGTAGCTGCTACCGCCAACCTGGTGGGGATCACCGGCATTGCCCGGCGCCTGGTCATGGACGGGGCGTTGGACGA
CAACGCGGCACGGCAGGCGCTCGCCGCCGCCACCGCGGCCAAGCAACCGATCACCGCCTACCTCGCCGAAAAACGTCTCG
TCACGCCAGGCCAGCTTGCGGCCGCCAACTCGATCGAGTTCGGCGTGCCGTTGTTCGATGCCACGGTGCTCGACGCTTCG
CAGTCGGCCATCAAGCTGGTCAGCGAATCGCTCGTCCGCAAGCATCAGATCCTGCCGCTGTACAAGCGCGGCAACCGCCT
GTTCGTTGGCGTCTCGGATCCGACCAACACGCTGGCGCTGGACGAAATCAAGTTCCAGAGCAACCTCGCGATCGAACCGA
TCCTGATCAGCGAGGACCAGATCCAGCGCTGCATCGAGAAGTGGCTGGAGGTCAGCGACAACCTCGCCGACACCGTCGGC
AACGACGAAGGGCTGGAGAACCTCGACATCTCCGGTGGCGATGACATCGCCGCCGACAACACCGTCGACGTCAAGACCGA
CGACACGCCGGTCGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCCGCGGCGCGTCGGACATCCACTTCGAGC
CCTACGAAACCGAATACCGCGTTCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCACCAAGGTGCCGACCAATCTGTCC
GGCCGCATCGCCGCGCGACTGAAGGTGATGTCGCAGCTCGACATCGCCGAAAAGCGCGTGCCGCAGGACGGCCGCATCAA
GCTCAACATCTCCAAGACCAAGCAGGTCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAAGTGGTGCTGC
GTATCCTCGACGGCAGCGCGGCCAAGCTCGGCATCGACAAGCTGGGTTACGAGGACGATCAGCGCGCGCTGTACGAACAG
GCGCTGGCCAAGCCTTACGGCATGGTGCTCGTCACCGGCCCCACCGGCTCCGGCAAGACGGTGTCGCTGTACACGGGCCT
GAACATCCTCAACGAGGAACAGCGCAACATCTCCACCGTCGAAGACCCGGTCGAAATCCGCGTGCCGGGCATCAACCAAG
TGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGACAGGACCCGGACGTGGTGATG
GTCGGCGAAATCCGCGACCTGGAGACGGCGGAGATCGCGGTGAAGGCCGCGCAGACCGGTCACCTCGTGCTCTCCACGCT
GCACACCAACGATGCCCCGCAGACCGTCTCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCTCGTCGGTGA
CGCTGATCATCGCCCAGCGCCTTGCGCGCCGACTGCACGACTGCAAGCAGGAAGTGCATCTGCCGGAGCACGCCCTGCTG
GCCGAAGGCTTCACCCACAAGGAAGTCGAAGCGGGCATCAAGATCTACGAGGCGGTCGGCTGCTCCGACTGCACCGAAGG
CTACAAGGGCCGCACCGGCATCTACCAGGTCATGCCGATGACCGAAAAGATCCAGGCCATCATCCTGCAGGGCGGCAACG
CGCTGGACATCGCCGAAGCCGCCAAGCAGTCCGGCGTGCGTGACCTGCGCATGTCGGCCCTGCTCAAAGTGAAGAACGGC
GTCACCAGCCTTGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.895

99.825

0.548
  pilB Acinetobacter baumannii D1279779

55.203

98.953

0.546
  pilB Legionella pneumophila strain ERS1305867

51.06

98.778

0.504
  pilB Vibrio cholerae strain A1552

52.838

89.18

0.471
  pilF Neisseria gonorrhoeae MS11

47.443

98.953

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

49.714

91.623

0.455
  pilB Vibrio campbellii strain DS40M4

51.852

84.817

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

35.541

100

0.384

Multiple sequence alignment