Detailed information NTG0979523

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: AAEO81_RS26815
• Genome accession: NZ_CP151650
• Coordinates: 5951337..5953031 (+)
• Length: 564 a.a.
• NCBI ID: WP_341960023.1
• Uniprot ID: -
• Organism: Pseudomonas sp. RC10
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5953034..5964703	5951337..5953031	flank	3

Gene organization within MGE regions

Location: 5951337..5964703

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAEO81_RS26815 (AAEO81_26815)	pilB	5951337..5953031 (+)	1695	WP_341960023.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AAEO81_RS26820 (AAEO81_26820)	pilC	5953034..5954251 (+)	1218	WP_341960024.1	type II secretion system F family protein	Machinery gene
AAEO81_RS26825 (AAEO81_26825)	pilD	5954254..5955126 (+)	873	WP_341960025.1	A24 family peptidase	Machinery gene
AAEO81_RS26830 (AAEO81_26830)	coaE	5955123..5955758 (+)	636	WP_341960027.1	dephospho-CoA kinase	-
AAEO81_RS26835 (AAEO81_26835)	yacG	5955755..5955958 (+)	204	WP_166597108.1	DNA gyrase inhibitor YacG	-
AAEO81_RS26840 (AAEO81_26840)	-	5956114..5956797 (-)	684	WP_341960031.1	energy-coupling factor ABC transporter permease	-
AAEO81_RS26845 (AAEO81_26845)	-	5956909..5957553 (-)	645	WP_341960034.1	hydrolase	-
AAEO81_RS26850 (AAEO81_26850)	-	5957709..5958608 (+)	900	WP_166597111.1	LysR family transcriptional regulator	-
AAEO81_RS26855 (AAEO81_26855)	-	5958668..5959621 (-)	954	WP_341960035.1	2-hydroxyacid dehydrogenase	-
AAEO81_RS26860 (AAEO81_26860)	-	5959654..5960418 (-)	765	WP_166597113.1	SDR family oxidoreductase	-
AAEO81_RS26865 (AAEO81_26865)	-	5960460..5961161 (-)	702	WP_341964630.1	fumarylacetoacetate hydrolase family protein	-
AAEO81_RS26870 (AAEO81_26870)	-	5961458..5962801 (-)	1344	WP_341960037.1	MFS transporter	-
AAEO81_RS26875 (AAEO81_26875)	-	5963124..5964026 (+)	903	WP_341960039.1	AraC family transcriptional regulator	-
AAEO81_RS26880 (AAEO81_26880)	-	5964146..5964703 (+)	558	WP_341960041.1	hypothetical protein	-

Sequence

Protein

• Length: 564 a.a.
• Molecular weight: 62264.50 Da
• Isoelectric Point: 5.8540

>NTDB_id=979523 AAEO81_RS26815 WP_341960023.1 5951337..5953031(+) (pilB) [Pseudomonas sp. RC10]
MTDVVLTGLAKQLVVAELLTENAAQQAYQQARRDKTSLVSYLVQNKLVNSLVLAEMASEQFGLPFLDLSTLDKDTQPRGL
VSEKLIRQHNALPLWRRGNKLFVGVSDPTNHQAITDIQFSTGLNTEAILVEDNKLTSAIEKLFDTHAGLGEMADVDLSLE
VESTDDVKETTLGEVDAEDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVAKPPTQLAGRIAARLKV
MAGLDISERRRPQDGRIKLRISKNRAIDFRINTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKTLYLEALSKPQGMI
LVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCPHCKKEIDIPRETLIAEGFPPDK
IGSFKLYSPVGCEHCNAGYKGRVGIYEVVKSTPALERIIMEEGNALDISVQMRKDGFNDLRTSGLSKAMQGLTSLEEINR
VTKD

Nucleotide

• Length: 1695 bp

>NTDB_id=979523 AAEO81_RS26815 WP_341960023.1 5951337..5953031(+) (pilB) [Pseudomonas sp. RC10]
ATGACTGATGTTGTCCTCACCGGCTTGGCCAAGCAATTGGTTGTTGCCGAACTCCTTACCGAAAACGCTGCACAGCAGGC
CTACCAACAGGCGCGCCGCGATAAAACCTCTCTCGTGAGCTACCTAGTCCAGAACAAGCTAGTTAACAGCCTTGTTTTGG
CAGAAATGGCCTCTGAACAGTTCGGCCTTCCTTTCCTTGACCTAAGTACGCTAGACAAAGACACCCAACCGAGAGGGCTG
GTGAGCGAAAAACTCATCCGTCAACACAATGCCTTACCTCTCTGGCGTCGAGGCAACAAGCTTTTCGTGGGTGTCTCCGA
TCCTACAAATCATCAGGCAATCACCGATATCCAGTTCAGCACTGGCCTGAATACCGAAGCGATTTTGGTCGAAGATAACA
AGCTGACGAGCGCTATCGAAAAACTTTTCGATACGCACGCCGGGCTTGGCGAAATGGCAGATGTGGATCTAAGCCTTGAA
GTAGAGAGTACTGATGATGTCAAAGAAACCACATTAGGTGAGGTTGATGCGGAAGACGCACCCGTTGTTCGTTTCGTTAA
CAAGATGTTGATGGATGCCATCCGCCTTGGCTCTTCTGACTTACATTTCGAACCTTATGAAAAAATCTTCCGAGTTCGTC
TACGTACGGATGGAATTCTTCACGAGGTCGCGAAACCTCCAACACAATTGGCCGGCAGGATCGCTGCGCGGTTAAAAGTA
ATGGCGGGGCTCGATATCTCTGAGCGCCGAAGACCGCAAGACGGCCGTATCAAGCTGCGAATCTCAAAAAATCGCGCGAT
AGATTTTCGTATCAACACGCTTCCCACATTGTGGGGGGAAAAAATTGTAATGCGGATACTGGATCCGACCAGTGCCCAGA
TGGGCATCGATGCATTGGGCTATGAACCGGATCAAAAAACCCTTTATCTTGAGGCACTGAGCAAACCTCAAGGCATGATA
CTAGTGACCGGCCCTACCGGTTCGGGAAAGACCGTCTCGTTATATACAGGTTTAAATATCCTCAACACCCCAGACATCAA
CATTTCCACGGCTGAAGACCCAGTCGAGATTAACCTTGAAGGGATTAACCAAGTCAATGTAAATCCCCGACAGGGGATGG
ATTTCTCGCAGGCTCTTCGCGCATTTCTCCGCCAAGATCCTGACGTAATTATGGTCGGAGAGATTCGTGATTTGGAGACC
GCTGAGATTGCGATAAAGGCATCCCAAACGGGCCATATGGTCCTGTCGACTCTTCATACCAACAGCGCGGCAGAAACGCT
GACCCGTTTGCATCATATGGGGGTGGCTGCTTTTAATATTGCAACTGCGATAAATTTGATCATTGCACAGCGCTTGGCGC
GAAAGCTTTGTCCTCACTGCAAAAAAGAAATCGACATTCCACGAGAAACATTGATTGCAGAAGGTTTCCCTCCAGACAAG
ATTGGCAGTTTCAAGCTTTACTCTCCTGTAGGGTGCGAGCACTGCAACGCAGGTTATAAAGGAAGAGTAGGGATCTACGA
GGTGGTTAAAAGTACCCCGGCGCTGGAACGCATAATTATGGAGGAAGGCAACGCCTTGGACATCTCGGTACAAATGCGCA
AGGACGGCTTCAATGATTTACGCACTTCCGGACTATCAAAAGCAATGCAAGGTTTGACTAGCCTTGAAGAAATCAACCGT
GTGACTAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (42-147)

• Type II/IV secretion system protein (T2SSE) (188-455)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.345	100	0.574
pilB	Acinetobacter baylyi ADP1	57.016	99.823	0.569
pilB	Legionella pneumophila strain ERS1305867	54.513	100	0.546
pilB	Vibrio cholerae strain A1552	51.327	100	0.514
pilB	Vibrio parahaemolyticus RIMD 2210633	50.817	97.695	0.496
pilB	Vibrio campbellii strain DS40M4	49.466	99.645	0.493
pilF	Neisseria gonorrhoeae MS11	47.594	99.468	0.473
pilF	Thermus thermophilus HB27	38.448	100	0.387