Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AABM54_RS02130 Genome accession   NZ_CP150918
Coordinates   471025..473115 (+) Length   696 a.a.
NCBI ID   WP_347903410.1    Uniprot ID   -
Organism   Pseudomonas purpurea strain DGS26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466025..478115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM54_RS02110 pilM 468237..469301 (+) 1065 WP_347903403.1 pilus assembly protein PilM Machinery gene
  AABM54_RS02115 - 469301..469867 (+) 567 WP_347903405.1 PilN domain-containing protein -
  AABM54_RS02120 pilO 469864..470487 (+) 624 WP_347903407.1 type 4a pilus biogenesis protein PilO -
  AABM54_RS02125 - 470484..471011 (+) 528 WP_347903409.1 pilus assembly protein PilP -
  AABM54_RS02130 pilQ 471025..473115 (+) 2091 WP_347903410.1 type IV pilus secretin PilQ Machinery gene
  AABM54_RS02135 aroK 473120..473638 (+) 519 WP_347903411.1 shikimate kinase AroK -
  AABM54_RS02140 aroB 473787..474887 (+) 1101 WP_347903412.1 3-dehydroquinate synthase -
  AABM54_RS02145 - 474898..476505 (+) 1608 WP_347903413.1 AAA family ATPase -

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 75221.37 Da        Isoelectric Point: 6.0192

>NTDB_id=973771 AABM54_RS02130 WP_347903410.1 471025..473115(+) (pilQ) [Pseudomonas purpurea strain DGS26]
MNRTLSSLGFSLWIALLSPMVHATSLKALDVAALPGDRVELKLTFDGPPPAAHGYTTEQPARIALDLPGVTNQLASKNRE
LGSGNARSATVVEAKDRTRLIINLAQSAPYNTRVEGNNLFVVVGQGVAAAPKPRVAAAPRVASATPAPAKAYVPVSKAIR
GVDFQRGEQGEGNVVIDLSDPGISPDIQERDGKIILSFANTQLPEPLRVRLDVKDFATPVQYVNASASPGKASISIEPSG
TFDYSTFQTDNKLTVSVRPMTVDDLQKRNADRHAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDDRTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVRNKGNWNTSGVS
NNGANTSSTIGTPGSTSTNSPFVDMGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEVVSQPKVVTSDKETAKILKGT
EIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGG
VFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=973771 AABM54_RS02130 WP_347903410.1 471025..473115(+) (pilQ) [Pseudomonas purpurea strain DGS26]
ATGAACAGGACCCTTTCATCCCTCGGTTTTTCGCTATGGATAGCGCTGTTGTCACCGATGGTCCACGCGACCAGTCTCAA
AGCGCTGGACGTCGCGGCGCTGCCGGGCGATCGCGTCGAGTTGAAGCTCACGTTCGACGGACCGCCGCCTGCGGCTCACG
GCTACACCACCGAGCAACCCGCGCGGATTGCCCTCGATCTGCCGGGCGTGACCAATCAATTGGCCAGCAAGAATCGCGAA
TTGGGCAGCGGCAACGCCCGCAGTGCGACAGTGGTCGAAGCCAAGGACCGTACCCGGTTGATCATTAACCTGGCGCAATC
GGCGCCGTACAACACGCGGGTCGAAGGCAACAATCTGTTCGTGGTGGTCGGGCAGGGGGTTGCTGCTGCGCCGAAACCTC
GCGTCGCGGCCGCGCCGCGAGTGGCCAGTGCCACACCCGCTCCAGCCAAGGCTTATGTCCCGGTGAGCAAGGCCATTCGT
GGCGTGGACTTCCAGCGTGGCGAGCAAGGCGAAGGTAACGTTGTGATCGACCTGTCGGACCCCGGCATCAGCCCGGATAT
CCAGGAGCGTGACGGCAAAATCATCCTCAGCTTTGCCAACACCCAATTGCCGGAGCCGTTGCGGGTGCGCCTGGACGTGA
AGGATTTCGCCACGCCGGTGCAATATGTCAACGCCAGTGCCTCCCCGGGCAAGGCCAGCATCAGCATCGAACCCAGCGGT
ACGTTCGATTACTCGACCTTCCAGACCGATAACAAGCTGACGGTCAGCGTCCGCCCAATGACCGTCGACGATCTGCAAAA
GCGCAATGCCGATCGTCATGCCTACACCGGCGAGAAGCTGTCGTTGAATTTCCAGGACATCGACGTGCGCTCGGTGCTGC
AGCTGATTGCCGACTTCACCAATCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGTCTGCAAAAC
GTACCCTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGATCGGCAACGTGTTGCTGGT
GGCGCCGGCGGATGAAATTGCCGCCCGTGAGCGTCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAACTCGCTCCGCTAC
GCCGGGAGCTGTTGCAGGTCAACTACGCCAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGCGCCGAAGCA
AAAATCGACGAACGCGGTTCGATCACCGTCGATGACCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
TGAGCTGCGCCGAATTGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCACGGATCGTCGAAGCCAACG
TCGATTACGACAAGAGCCTGGGGGTCCGTTGGGGTGGTTCGGTGCGCAACAAGGGCAACTGGAACACCTCCGGCGTCAGC
AATAACGGGGCGAACACTTCCTCGACCATCGGTACGCCGGGCAGCACGAGTACCAACTCGCCGTTCGTCGACATGGGCGC
GGCGGGCAATACCTCGGGCATCGGCATCGCCTTTATCACGGACAACGTGTTGCTGGACCTCGAACTGACGGCGATGGAGA
AAACCGGCAACGGCGAAGTGGTCTCCCAGCCCAAGGTGGTCACCTCGGACAAGGAAACCGCCAAGATCCTCAAGGGCACA
GAGATTCCCTACCAGGAAGCCAGCTCCAGCGGCGCCACTTCGGTGTCCTTCAAGGAGGCTTCGCTGTCGTTGGAAGTGAC
CCCGCAGATCACCCCCGACAACCGCATCATCATGGAGGTGAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGC
AGGACGTACCGCCAATCAAGAAGAACGAGGTCAACGCCAAGGTGTTGGTCAATGATGGCGAGACGATCGTCATCGGCGGG
GTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCG
GCGTGACGTGGTTTCGGAGAAAAAATCCGAGCTGTTGGTGTTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTG
TGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(531-687)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.266

100

0.763
  comQ Acinetobacter baylyi ADP1

41.066

99.713

0.409
  pilQ Acinetobacter baumannii D1279779

37.551

100

0.397
  pilQ Acinetobacter baumannii strain A118

37.551

100

0.397
  pilQ Legionella pneumophila strain Lp02

38.71

97.989

0.379
  pilQ Legionella pneumophila strain ERS1305867

38.586

97.557

0.376

Multiple sequence alignment