Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AABM56_RS19800 Genome accession   NZ_CP150916
Coordinates   4274234..4276489 (-) Length   751 a.a.
NCBI ID   WP_263259283.1    Uniprot ID   -
Organism   Pseudomonas aestiva strain DGS32     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4269234..4281489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM56_RS19785 - 4270946..4272544 (-) 1599 WP_133861487.1 AAA family ATPase -
  AABM56_RS19790 aroB 4272563..4273657 (-) 1095 WP_133861488.1 3-dehydroquinate synthase -
  AABM56_RS19795 aroK 4273710..4274228 (-) 519 WP_133861489.1 shikimate kinase AroK -
  AABM56_RS19800 pilQ 4274234..4276489 (-) 2256 WP_263259283.1 type IV pilus secretin PilQ Machinery gene
  AABM56_RS19805 - 4276490..4277008 (-) 519 WP_133861491.1 pilus assembly protein PilP -
  AABM56_RS19810 pilO 4277005..4277658 (-) 654 WP_133861492.1 type 4a pilus biogenesis protein PilO -
  AABM56_RS19815 - 4277655..4278245 (-) 591 WP_133861493.1 PilN domain-containing protein -
  AABM56_RS19820 comM 4278246..4279310 (-) 1065 WP_133861494.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 751 a.a.        Molecular weight: 80021.79 Da        Isoelectric Point: 9.1482

>NTDB_id=973734 AABM56_RS19800 WP_263259283.1 4274234..4276489(-) (pilQ) [Pseudomonas aestiva strain DGS32]
MGRASTVNTTIAGRLGLGLGLALLLSSLAGQALAADLQALNVASLPGDRIELKLTFDQPVPVPKGYTIEQPARIALDLPG
VANKLGTKSRELGNGNARSVTVVEAGNRTRLIINLTNLASYDTRADGRNLFVTIGGTAGRQTASAQANLTPNLPRAAAPA
TVTAPSSAALPGAVARTPLPPTQATSTVAAMTAPRPQLRGKAIRHIDFQRGPQGEGNVVIDLSETGINPDIQEQGGKIRL
TFAGTQLPEELRVRLDVKDFATPVQFVNASADSGGASIVIEPTGTYDYLAYQTNNRLTLSVKPLTTEEKEKRAADRFTYT
GEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGNITLRLQNVPWDQALDLILKTKGLDKRQIGNVLLVAPADEIAAR
ERQELESQKQIQQLAPLRRDLIQVNYAKAADIAKLFQSVTSADKTADERGSISVDERTNSIIAYQTQNRLDELRRIVSQL
DIPVRQVMIEARLVEANVDYSKQLGVRWGGNASSGNFAVYGKDGATTVDSTTGQVFLPGTSTVGSFTSASGVAPTPFVDM
GVSGSTSGIGVGFMVGGKMLDLQLSALQSSGNGEIISQPKVVTSDKETAKILRGTEIPYQEASSSGATSTSFKQAALSLE
VTPQITPDNRIIMEVKVNKDAPDYQNALNGVPPISKNEVNAKVLVSDGETIVLGGVFSNTQTKSTTKVPMLGDIPYVGRL
FRRDSVTDSKVELLIFLTPRIMNNQAIAVGR

Nucleotide


Download         Length: 2256 bp        

>NTDB_id=973734 AABM56_RS19800 WP_263259283.1 4274234..4276489(-) (pilQ) [Pseudomonas aestiva strain DGS32]
ATGGGCAGGGCGAGCACTGTGAACACTACTATCGCGGGACGACTGGGACTGGGACTGGGACTGGCCCTGCTGCTGAGCAG
CCTGGCCGGTCAGGCCCTGGCAGCCGATCTGCAGGCCTTGAACGTCGCCTCCCTGCCCGGGGATCGCATCGAACTCAAGC
TGACCTTCGATCAGCCGGTGCCGGTGCCCAAGGGGTACACCATAGAGCAGCCGGCGCGGATTGCCCTGGATCTGCCCGGC
GTCGCCAACAAGCTGGGTACCAAGAGCCGCGAGCTGGGCAATGGCAATGCTCGCAGCGTGACGGTGGTCGAGGCGGGCAA
TCGCACCCGCCTGATCATCAACCTCACCAACCTGGCCAGCTACGACACCCGGGCCGACGGCCGCAATCTGTTCGTCACCA
TCGGCGGCACCGCCGGTCGCCAGACTGCCAGCGCCCAGGCCAACCTGACGCCCAACCTGCCGCGCGCCGCCGCGCCGGCC
ACCGTGACCGCACCCTCGTCCGCCGCCCTGCCTGGCGCGGTCGCCCGCACGCCGCTGCCGCCGACCCAGGCCACCTCCAC
CGTGGCGGCCATGACCGCGCCGCGGCCGCAACTGCGCGGCAAGGCGATCCGCCATATCGATTTCCAGCGGGGTCCCCAGG
GCGAGGGCAACGTAGTCATCGACCTGAGCGAGACCGGCATCAACCCGGACATCCAGGAGCAGGGCGGCAAGATCCGCCTG
ACCTTCGCCGGTACCCAGCTGCCCGAAGAGCTGCGGGTGCGCCTGGACGTCAAGGACTTCGCCACCCCCGTGCAGTTCGT
CAACGCCTCCGCTGACAGCGGCGGGGCGTCCATCGTCATCGAGCCGACCGGGACCTACGACTACCTGGCCTACCAGACCA
ACAATCGTCTGACCTTGAGCGTCAAGCCGCTCACCACCGAGGAAAAGGAAAAGCGCGCAGCCGATCGCTTCACCTATACC
GGCGAGAAACTGTCGCTGAATTTCCAGGACATCGACGTCCGCTCGGTGCTGCAGCTGATCGCCGACTTCACCAACCTCAA
CCTGGTGGCGAGCGACACCGTGCAGGGCAACATCACCCTGCGCCTGCAGAACGTGCCCTGGGACCAGGCGCTGGATCTGA
TCCTCAAGACCAAGGGTCTCGACAAGCGCCAGATCGGCAACGTGCTGCTGGTTGCACCGGCCGACGAGATCGCCGCGCGC
GAGCGTCAGGAGCTGGAATCCCAGAAGCAGATCCAGCAGCTGGCCCCGCTGCGTCGCGACCTGATCCAGGTCAACTACGC
CAAGGCCGCCGATATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGACAAGACCGCCGACGAGCGCGGCTCCATCTCGG
TGGACGAGCGCACCAACAGCATCATTGCCTACCAGACCCAGAATCGCCTGGACGAACTCCGGCGCATCGTCTCGCAGCTG
GATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGCTGGTGGAGGCCAACGTCGACTACAGCAAGCAGCTGGGCGTGCG
CTGGGGCGGTAACGCCAGTTCGGGCAACTTCGCGGTCTATGGTAAGGACGGCGCCACCACCGTCGATTCCACGACCGGTC
AGGTGTTCCTGCCGGGGACCAGTACCGTAGGCAGCTTCACCTCCGCCAGTGGTGTGGCGCCCACGCCCTTCGTCGACATG
GGCGTTTCCGGCAGCACCTCGGGCATCGGTGTCGGCTTCATGGTCGGTGGCAAGATGCTCGACCTGCAGCTGTCCGCGCT
GCAATCCAGCGGCAATGGCGAGATCATCTCCCAGCCCAAGGTGGTGACCTCGGACAAGGAGACCGCCAAGATCCTGCGCG
GTACCGAGATCCCCTATCAGGAAGCCAGTTCCAGCGGTGCCACCAGCACCAGCTTCAAGCAGGCCGCCCTGTCGCTGGAG
GTCACGCCGCAGATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCAACAAGGACGCCCCTGACTACCAGAACGC
CCTCAACGGTGTGCCGCCGATCAGCAAGAACGAGGTCAATGCCAAGGTGCTGGTGAGCGATGGCGAGACCATCGTGCTGG
GCGGGGTGTTCTCTAATACCCAGACCAAGTCCACCACCAAGGTGCCGATGCTGGGGGATATTCCCTATGTCGGCCGACTG
TTCCGCCGGGATTCGGTCACCGACAGCAAAGTCGAGCTGCTGATCTTCCTCACGCCGCGTATCATGAACAACCAGGCGAT
AGCGGTTGGTCGATGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(49-134)

Secretin and TonB N terminus short domain (STN)

(347-393)

Bacterial type II/III secretion system short domain (Secretin_N)

(422-486)

Bacterial type II and III secretion system protein (Secretin)

(586-742)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.285

98.136

0.69
  comQ Acinetobacter baylyi ADP1

40.454

99.734

0.403
  pilQ Acinetobacter baumannii D1279779

39.366

100

0.397
  pilQ Acinetobacter baumannii strain A118

39.366

100

0.397

Multiple sequence alignment