Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WOB24_RS14990 Genome accession   NZ_CP150891
Coordinates   3186984..3188687 (+) Length   567 a.a.
NCBI ID   WP_341303785.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP25     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3181984..3193687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOB24_RS14955 (WOB24_14955) nadC 3182203..3183051 (+) 849 WP_341303778.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WOB24_RS14960 (WOB24_14960) - 3183140..3184048 (-) 909 WP_341303779.1 ABC transporter ATP-binding protein -
  WOB24_RS14965 (WOB24_14965) - 3184048..3184761 (-) 714 WP_341303780.1 hypothetical protein -
  WOB24_RS14970 (WOB24_14970) - 3184886..3185695 (-) 810 WP_341303781.1 ABC transporter permease -
  WOB24_RS14975 (WOB24_14975) - 3185800..3186003 (-) 204 WP_341303782.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WOB24_RS14980 (WOB24_14980) - 3186098..3186541 (-) 444 WP_341303783.1 hypothetical protein -
  WOB24_RS14985 (WOB24_14985) - 3186561..3186746 (-) 186 WP_341303784.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WOB24_RS14990 (WOB24_14990) pilB 3186984..3188687 (+) 1704 WP_341303785.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WOB24_RS14995 (WOB24_14995) pilC 3188691..3189911 (+) 1221 WP_341303786.1 type II secretion system F family protein Machinery gene
  WOB24_RS15000 (WOB24_15000) pilD 3189915..3190781 (+) 867 WP_341303787.1 A24 family peptidase Machinery gene
  WOB24_RS15005 (WOB24_15005) coaE 3191034..3191648 (+) 615 WP_341303788.1 dephospho-CoA kinase -
  WOB24_RS15010 (WOB24_15010) yacG 3191645..3191845 (+) 201 WP_341303789.1 DNA gyrase inhibitor YacG -
  WOB24_RS15015 (WOB24_15015) - 3191907..3192122 (-) 216 WP_341303790.1 hypothetical protein -
  WOB24_RS15020 (WOB24_15020) - 3192227..3192916 (-) 690 WP_339080105.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62280.23 Da        Isoelectric Point: 5.7993

>NTDB_id=973530 WOB24_RS14990 WP_341303785.1 3186984..3188687(+) (pilB) [Pseudomonas sp. TMP25]
MNEAPNLTGLARQLVNADLLSESAAAQAQAQALRNKIPLVAYLIQNKLAKSRAVAEIASEQFGVPFCDLSAIDKDSQPRD
LISEKLVRQHRVIPLFRRGNKLFVGVSDPTNHQAVTDVQFSTGLTTEPLLVEDDKLNDAIEKFFDSASSGLEELGDVDLD
GIDVEAVNDNEKDSNDGNSADDAPVVRFVHKMLLDAIKKGSSDLHFEPYEKTYRVRFRTDGILQEIARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMRISKTKSIDFRVSTCPTLWGEKIVMRILDSASAQMGIDALGYEESQKDLYMAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFTAALKSFLRQDPDIIMVGEIRD
LDTASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCSHCKKEVKVPREALLEEGFP
ADKVDTFKLYGPVGCENCNGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDIAVQMRKDGFNDLRTSALLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=973530 WOB24_RS14990 WP_341303785.1 3186984..3188687(+) (pilB) [Pseudomonas sp. TMP25]
ATGAATGAAGCCCCTAACCTGACAGGCTTGGCCCGGCAACTGGTCAATGCCGATCTGCTAAGCGAAAGTGCCGCCGCTCA
AGCCCAAGCTCAGGCGCTTCGCAATAAAATCCCGCTGGTTGCCTACCTAATACAAAACAAGCTTGCGAAAAGTAGAGCCG
TCGCAGAAATAGCCTCTGAACAGTTTGGCGTGCCCTTTTGCGACCTAAGCGCCATTGACAAAGACAGCCAGCCACGCGAC
CTGATCAGTGAAAAGCTGGTGCGCCAACATCGGGTTATCCCGCTATTTCGCCGCGGCAATAAACTCTTTGTTGGGGTCTC
CGACCCCACCAACCATCAAGCGGTTACTGACGTACAATTTTCCACCGGCCTGACCACTGAGCCACTGCTGGTGGAAGATG
ACAAGCTGAATGACGCCATTGAGAAGTTCTTTGACTCCGCCAGTAGCGGCCTAGAAGAACTGGGCGATGTAGACCTCGAC
GGAATAGATGTTGAGGCGGTCAATGATAACGAGAAGGACAGCAACGACGGTAATTCGGCTGACGACGCACCGGTTGTGCG
CTTTGTACACAAGATGCTGTTGGATGCGATCAAGAAGGGCTCATCCGACCTGCACTTCGAACCTTATGAAAAAACTTATC
GGGTGCGCTTTCGCACTGACGGTATTTTGCAGGAAATAGCCCGCCCCCCTATCCAGCTGGCCCCGCGTATTTCCGCGCGC
CTTAAAGTAATGGCCGGGCTGGATATTTCCGAACGGCGCAAACCGCAGGATGGGCGCATTAAAATGCGCATCTCCAAAAC
CAAGTCCATCGACTTTCGCGTCAGCACCTGCCCAACATTGTGGGGTGAGAAGATCGTGATGCGGATTCTCGATTCCGCCA
GCGCACAAATGGGTATTGATGCCCTAGGCTATGAGGAGTCACAGAAAGACCTTTATATGGCTGCACTCAAGCAACCCCAG
GGCATGATTCTGGTAACCGGCCCCACCGGCTCTGGCAAAACTGTGTCGCTGTATACCGGCCTGAACATCCTCAACACCGT
GGACGTGAATATCTCCACGGCCGAAGACCCGGTTGAAATCAACCTGGAAGGCATTAACCAGGTCAACGTAAACCCTAAGC
AGGGCATGGACTTTACTGCTGCACTGAAATCATTCCTGCGACAAGACCCAGACATCATCATGGTCGGGGAAATTCGTGAC
TTGGACACCGCCTCTATTGCGATCAAGGCCGCACAAACCGGGCACATGGTGATGTCCACCTTGCACACCAACAGCGCCGC
AGAAACCCTGACGCGCCTGCGCAACATGGGCGTGCCGTCGTTTAACATCGCCACCTCGGTGAATCTGATTATCGCCCAGC
GCCTAGCGCGTAAGCTGTGCAGCCACTGCAAAAAAGAAGTGAAGGTGCCGCGTGAAGCACTTCTTGAAGAAGGCTTCCCG
GCGGACAAGGTCGATACATTTAAGCTTTATGGCCCAGTAGGCTGCGAAAACTGCAACGGCGGCTACAAAGGTCGTGTCGG
AATATATGAAGTGGTTAAAAACACGCCTGCCCTGCAACGGATTATCATGGAGGAAGGCAATTCCATTGATATTGCCGTGC
AAATGCGTAAAGACGGCTTCAATGACCTGCGCACATCCGCTTTACTGAAAGCAATGCAGGGCATTACCAGCCTTGAAGAA
GTCAACCGAGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.464

100

0.591
  pilB Acinetobacter baylyi ADP1

58.23

99.647

0.58
  pilB Legionella pneumophila strain ERS1305867

54.211

100

0.545
  pilF Neisseria gonorrhoeae MS11

50.177

99.824

0.501
  pilB Vibrio cholerae strain A1552

50.178

99.118

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

49.735

99.647

0.496
  pilB Vibrio campbellii strain DS40M4

48.932

99.118

0.485
  pilB/pilB1 Synechocystis sp. PCC 6803

39.885

92.416

0.369

Multiple sequence alignment