Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VT188_RS26505 Genome accession   NZ_CP177358
Coordinates   5687113..5687736 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain CWC0815     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5682113..5692736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VT188_RS26485 (VT188_026485) aroB 5682708..5683814 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  VT188_RS26490 (VT188_026490) aroK 5683864..5684382 (-) 519 WP_003095833.1 shikimate kinase AroK -
  VT188_RS26495 (VT188_026495) pilQ 5684394..5686538 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  VT188_RS26500 (VT188_026500) comL/pilP 5686592..5687116 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  VT188_RS26505 (VT188_026505) pilO 5687113..5687736 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  VT188_RS26510 (VT188_026510) pilN 5687733..5688329 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  VT188_RS26515 (VT188_026515) pilM 5688329..5689393 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  VT188_RS26520 (VT188_026520) - 5689578..5692049 (+) 2472 WP_017148635.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=970529 VT188_RS26505 WP_003103268.1 5687113..5687736(-) (pilO) [Pseudomonas aeruginosa strain CWC0815]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=970529 VT188_RS26505 WP_003103268.1 5687113..5687736(-) (pilO) [Pseudomonas aeruginosa strain CWC0815]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401