Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VT188_RS26510 Genome accession   NZ_CP177358
Coordinates   5687733..5688329 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain CWC0815     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5682733..5693329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VT188_RS26490 (VT188_026490) aroK 5683864..5684382 (-) 519 WP_003095833.1 shikimate kinase AroK -
  VT188_RS26495 (VT188_026495) pilQ 5684394..5686538 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  VT188_RS26500 (VT188_026500) comL/pilP 5686592..5687116 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  VT188_RS26505 (VT188_026505) pilO 5687113..5687736 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  VT188_RS26510 (VT188_026510) pilN 5687733..5688329 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  VT188_RS26515 (VT188_026515) pilM 5688329..5689393 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  VT188_RS26520 (VT188_026520) - 5689578..5692049 (+) 2472 WP_017148635.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=970530 VT188_RS26510 WP_003095839.1 5687733..5688329(-) (pilN) [Pseudomonas aeruginosa strain CWC0815]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=970530 VT188_RS26510 WP_003095839.1 5687733..5688329(-) (pilN) [Pseudomonas aeruginosa strain CWC0815]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369