Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OO966_RS02225 Genome accession   NZ_AP026780
Coordinates   448076..449182 (+) Length   368 a.a.
NCBI ID   WP_264954827.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain 04Ya311     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443076..454182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO966_RS02195 (PDY_03980) - 443912..444505 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  OO966_RS02200 (PDY_03990) yggU 444607..444906 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  OO966_RS02205 (PDY_04000) - 444903..445460 (-) 558 WP_005297682.1 YggT family protein -
  OO966_RS02210 (PDY_04010) proC 445472..446290 (-) 819 WP_005297684.1 pyrroline-5-carboxylate reductase -
  OO966_RS02215 (PDY_04020) - 446306..447007 (-) 702 WP_005297686.1 YggS family pyridoxal phosphate-dependent enzyme -
  OO966_RS02220 (PDY_04030) pilT 447030..448064 (+) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  OO966_RS02225 (PDY_04040) pilU 448076..449182 (+) 1107 WP_264954827.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OO966_RS02230 (PDY_04050) ruvX 449510..449935 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  OO966_RS02235 (PDY_04060) - 449932..450495 (-) 564 WP_264954828.1 YqgE/AlgH family protein -
  OO966_RS02240 (PDY_04070) gshB 450649..451596 (-) 948 WP_005297697.1 glutathione synthase -
  OO966_RS02245 (PDY_04080) rsmE 451617..452348 (-) 732 WP_264954829.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OO966_RS02250 (PDY_04090) endA 452480..453184 (-) 705 WP_264954830.1 deoxyribonuclease I -
  OO966_RS02255 (PDY_04100) - 453315..453797 (-) 483 WP_036763215.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41386.57 Da        Isoelectric Point: 5.5884

>NTDB_id=96946 OO966_RS02225 WP_264954827.1 448076..449182(+) (pilU) [Photobacterium damselae subsp. damselae strain 04Ya311]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGMLHPIGDTLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=96946 OO966_RS02225 WP_264954827.1 448076..449182(+) (pilU) [Photobacterium damselae subsp. damselae strain 04Ya311]
ATGAAACTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCAGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGTATGTTGCATCCCATTGGCGATACGTTAGATCGTACAGGGGTTGATTTACTGTTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGGGGCGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGTGCAACGGGGTCGGGTA
AATCGACTTCGATGGCGGCAATGACCGGGTTTAGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGTCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTACGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCGGAAACGGGTCATTTATGTATGGCCACATTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGCATTCTGCAT
CTGGTACCGAAAGAGCGCCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCTGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACTAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTTACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

99.728

0.679
  pilU Pseudomonas stutzeri DSM 10701

59.497

97.283

0.579
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

93.75

0.389
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367

Multiple sequence alignment