Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CTR2_RS03940 Genome accession   NZ_AP026738
Coordinates   866483..868225 (-) Length   580 a.a.
NCBI ID   WP_087084998.1    Uniprot ID   -
Organism   Comamonas thiooxydans strain R2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 861483..873225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTR2_RS03910 (CTR2_R07570) - 862002..862472 (+) 471 WP_087085003.1 NUDIX domain-containing protein -
  CTR2_RS03915 (CTR2_R07580) - 862590..862799 (-) 210 WP_034357520.1 DNA gyrase inhibitor YacG -
  CTR2_RS03920 (CTR2_R07590) zapD 862899..863654 (-) 756 WP_087085002.1 cell division protein ZapD -
  CTR2_RS03925 (CTR2_R07600) coaE 863742..864368 (-) 627 WP_087085001.1 dephospho-CoA kinase -
  CTR2_RS03930 (CTR2_R07610) - 864372..865241 (-) 870 WP_087085000.1 A24 family peptidase -
  CTR2_RS03935 (CTR2_R07620) pilC 865241..866458 (-) 1218 WP_087084999.1 type II secretion system F family protein Machinery gene
  CTR2_RS03940 (CTR2_R07630) pilB 866483..868225 (-) 1743 WP_087084998.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CTR2_RS03950 (CTR2_R07640) - 868479..869408 (-) 930 WP_003062198.1 polyprenyl synthetase family protein -
  CTR2_RS03955 (CTR2_R07650) rplU 869751..870062 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  CTR2_RS03960 (CTR2_R07660) rpmA 870079..870336 (+) 258 WP_003058534.1 50S ribosomal protein L27 -
  CTR2_RS03965 (CTR2_R07670) cgtA 870493..871608 (+) 1116 WP_087084997.1 Obg family GTPase CgtA -
  CTR2_RS03970 (CTR2_R07680) proB 871719..872858 (+) 1140 WP_087084996.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63058.28 Da        Isoelectric Point: 5.1537

>NTDB_id=96774 CTR2_RS03940 WP_087084998.1 866483..868225(-) (pilB) [Comamonas thiooxydans strain R2]
MAAADTLQKNAPAPIAIPGLGRALISAGKVSQQAAENAAKKAATGKTPFITELSQSGEISALEIAETISMMFSTPLLDLN
AIDAQQLPRELLDPKISNAYRVLALSKRGNRLTIATADPTQQEAAEQIKFTTQLLVDWVVVEADKLQKLVEQTGKSVSES
LDSYSSSGDFDFDDVKIEDAPEEKDNAIGAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIAS
PPIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KERLLKAINRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCAQCKEPAD
IPHDALIDAGYDEAELDGSWVSYKPVGCPACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAAQAKAEGVRSLRGSG
LHKVKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=96774 CTR2_RS03940 WP_087084998.1 866483..868225(-) (pilB) [Comamonas thiooxydans strain R2]
ATGGCTGCTGCCGACACTCTGCAAAAGAACGCCCCTGCGCCCATTGCCATCCCGGGTTTAGGCAGGGCCCTGATTTCTGC
AGGAAAGGTTTCACAGCAAGCTGCCGAGAACGCAGCCAAGAAAGCCGCCACAGGCAAGACGCCGTTCATCACCGAACTGA
GCCAGTCCGGCGAGATCAGCGCGCTGGAAATTGCCGAAACCATTTCCATGATGTTCAGCACACCGCTGCTGGACCTCAAC
GCCATCGACGCCCAGCAGCTGCCGCGCGAGCTGCTGGACCCGAAAATCAGCAACGCCTATCGCGTGCTGGCACTCAGCAA
GCGCGGCAACCGGCTCACCATTGCCACGGCAGACCCCACGCAGCAGGAAGCGGCCGAGCAGATCAAGTTCACCACCCAGC
TGCTGGTGGACTGGGTGGTTGTCGAGGCCGACAAGCTGCAAAAGCTGGTCGAGCAGACCGGCAAGAGCGTCAGCGAATCC
CTGGACTCCTATTCCAGCTCCGGAGACTTTGACTTCGACGACGTCAAGATCGAGGATGCCCCCGAGGAAAAAGACAATGC
CATCGGCGCCGAGGTCGAGGATGCGCCGGTCGTCAAGTTTTTGCACAAGATGCTGCTGGACGCCTTCAATATGCGCGCGT
CCGACCTGCACTTCGAGCCCTACGAACACAATTACCGCGTGCGCTTTCGTATCGACGGCGAGCTGCGCGAGATTGCCTCG
CCCCCGATTGCGATCAAGGACAAGCTGGCTTCCCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAGAAACGCGTTCC
GCAAGACGGCCGCATGAAGCTCAAGGTCGGCCCTGATCGCGTCATCGACTTTCGTGTCAGCACCTTGCCCACGCTGTTTG
GCGAAAAGATCGTGATCCGTATTCTGGACCCGAGTTCGGCCAAGATGGGCATTGATGCCCTCGGCTACGAACCCGAGGAA
AAAGAGCGCCTGCTCAAGGCCATCAACCGCCCCTACGGCATGATTCTGGTCACCGGCCCCACGGGCTCGGGCAAGACCGT
GTCGCTCTATACCTGTCTGAACCTGCTCAACCAGCCGGGCGTGAACATTGCCACCGCCGAAGACCCTTCCGAAATCAACA
TGCCCGGCGTCAACCAGGTGAATGTGAATGAGAAGGCCGGCCTGACCTTTGCCGCTGCGCTCAAGTCCTTTCTGCGCCAG
GATCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCCCAGACCGGGCA
CCTGGTGCTGTCCACGCTGCACACCAATGACGCTCCGACCACGCTGACGCGCATGCGCAATATGGGCATCGCCCCTTTCA
ATATTGCCTCCAGCGTGATTCTGATCACCGCCCAGCGTCTGGCGCGACGCCTGTGTGCGCAGTGCAAGGAGCCCGCCGAC
ATACCGCACGACGCCCTGATCGATGCTGGCTACGATGAAGCCGAGCTCGACGGCAGCTGGGTCAGCTACAAGCCTGTCGG
CTGCCCTGCCTGCAACAACGGCTACAAGGGACGCGTCGGTATCTATCAGGTGATGCCCATCAGCGAAGAGATTCAGCGCA
TCATCCTGCGTGACGGCAGCGCCCTGGAGATTGCCGCACAGGCCAAGGCCGAAGGAGTACGCTCTCTGCGCGGCTCGGGC
CTGCATAAAGTCAAGCTGGGTCTGACCTCACTCGAAGAAGTGCTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.435

99.138

0.54
  pilB Acinetobacter baumannii D1279779

55.203

97.759

0.54
  pilF Neisseria gonorrhoeae MS11

52.473

97.586

0.512
  pilB Legionella pneumophila strain ERS1305867

50.883

97.586

0.497
  pilB Vibrio cholerae strain A1552

46.898

94.483

0.443
  pilB Vibrio campbellii strain DS40M4

44.029

96.724

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

46.008

90.69

0.417
  pilF Thermus thermophilus HB27

37.585

100

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.246

98.276

0.376

Multiple sequence alignment