Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCZ21_RS18330 Genome accession   NZ_AP026375
Coordinates   3975393..3976502 (+) Length   369 a.a.
NCBI ID   WP_042882153.1    Uniprot ID   A0A2L0TPJ0
Organism   Aeromonas caviae strain KAM497     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3970393..3981502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCZ21_RS18295 (KAM497c_35420) - 3970428..3971258 (+) 831 WP_244072973.1 CPBP family intramembrane glutamic endopeptidase -
  OCZ21_RS18300 (KAM497c_35430) - 3971334..3971753 (-) 420 WP_049636470.1 DUF4426 domain-containing protein -
  OCZ21_RS18305 (KAM497c_35440) yggU 3971772..3972071 (-) 300 WP_010673054.1 DUF167 family protein YggU -
  OCZ21_RS18310 (KAM497c_35450) - 3972071..3972622 (-) 552 WP_244072974.1 YggT family protein -
  OCZ21_RS18315 (KAM497c_35460) proC 3972646..3973470 (-) 825 WP_244072975.1 pyrroline-5-carboxylate reductase -
  OCZ21_RS18320 (KAM497c_35470) - 3973594..3974295 (-) 702 WP_244072977.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCZ21_RS18325 (KAM497c_35480) pilT 3974335..3975369 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  OCZ21_RS18330 (KAM497c_35490) pilU 3975393..3976502 (+) 1110 WP_042882153.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCZ21_RS18335 (KAM497c_35500) yaaA 3976535..3977308 (+) 774 WP_244072979.1 peroxide stress protein YaaA -
  OCZ21_RS18340 (KAM497c_35510) srmB 3977496..3978716 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  OCZ21_RS18345 (KAM497c_35520) - 3978844..3979554 (+) 711 WP_203764123.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  OCZ21_RS18350 (KAM497c_35530) brnQ 3979744..3981051 (+) 1308 WP_039040015.1 branched-chain amino acid transport system II carrier protein -
  OCZ21_RS18355 (KAM497c_35540) - 3981141..3981368 (-) 228 WP_039039956.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40901.04 Da        Isoelectric Point: 6.3155

>NTDB_id=96158 OCZ21_RS18330 WP_042882153.1 3975393..3976502(+) (pilU) [Aeromonas caviae strain KAM497]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=96158 OCZ21_RS18330 WP_042882153.1 3975393..3976502(+) (pilU) [Aeromonas caviae strain KAM497]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGACCAGCCCCCCTCGACAAGACGACAGCGCTGGCGCTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTCCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATTGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGACACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TPJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment