Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WJ399_RS26165 Genome accession   NZ_CP149513
Coordinates   5582302..5584002 (+) Length   566 a.a.
NCBI ID   WP_023094327.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2023CK-01620     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5577302..5589002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ399_RS26140 (WJ399_26140) ampD 5577313..5577879 (-) 567 WP_023096121.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WJ399_RS26145 (WJ399_26145) - 5578030..5580285 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  WJ399_RS26150 (WJ399_26150) nadC 5580571..5581419 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WJ399_RS26160 (WJ399_26160) pilA 5581618..5582070 (-) 453 WP_031636800.1 pilin Machinery gene
  WJ399_RS26165 (WJ399_26165) pilB 5582302..5584002 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WJ399_RS26170 (WJ399_26170) pilC 5584006..5585226 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  WJ399_RS26175 (WJ399_26175) pilD 5585230..5586102 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  WJ399_RS26180 (WJ399_26180) coaE 5586099..5586710 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  WJ399_RS26185 (WJ399_26185) yacG 5586707..5586907 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  WJ399_RS26190 (WJ399_26190) - 5586944..5587153 (-) 210 WP_003094660.1 hypothetical protein -
  WJ399_RS26195 (WJ399_26195) - 5587259..5587948 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  WJ399_RS26200 (WJ399_26200) - 5587945..5588415 (-) 471 WP_023096122.1 hypothetical protein -
  WJ399_RS26205 (WJ399_26205) - 5588412..5588837 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62399.27 Da        Isoelectric Point: 5.7041

>NTDB_id=959836 WJ399_RS26165 WP_023094327.1 5582302..5584002(+) (pilB) [Pseudomonas aeruginosa strain 2023CK-01620]
MNDSIQLSGLSRQLVQANLLDEKTALQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
TISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=959836 WJ399_RS26165 WP_023094327.1 5582302..5584002(+) (pilB) [Pseudomonas aeruginosa strain 2023CK-01620]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCTCTGCA
GGCGCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGTATCGCCTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
ACCATCAGCGAAAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTAGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGACGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.517

100

0.581
  pilB Acinetobacter baylyi ADP1

57.194

99.47

0.569
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

50.887

99.647

0.507
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio cholerae strain A1552

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.645

99.647

0.495
  pilF Thermus thermophilus HB27

40.073

96.996

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.19

92.756

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

38.951

94.346

0.367