Detailed information NTG0956610

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLI816_RS07030
• Genome accession: NZ_CP149095
• Coordinates: 1370979..1371626 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain I8-16
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1365979..1376626

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLI816_RS07005 (LLI816_07005)	atpF	1366050..1366556 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LLI816_RS07010 (LLI816_07010)	atpB	1366571..1367284 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLI816_RS07015 (LLI816_07015)	-	1367329..1367544 (-)	216	WP_260312241.1	F0F1 ATP synthase subunit C	-
LLI816_RS07020 (LLI816_07020)	-	1367728..1368504 (-)	777	WP_063283279.1	alpha/beta hydrolase family protein	-
LLI816_RS07025 (LLI816_07025)	-	1368789..1370998 (-)	2210	Protein_1360	DNA internalization-related competence protein ComEC/Rec2	-
LLI816_RS07030 (LLI816_07030)	comEA	1370979..1371626 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
LLI816_RS07035 (LLI816_07035)	-	1371686..1373047 (-)	1362	WP_021214597.1	ABC transporter permease	-
LLI816_RS07040 (LLI816_07040)	-	1373044..1373976 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLI816_RS07045 (LLI816_07045)	-	1374081..1374479 (-)	399	WP_004255236.1	hypothetical protein	-
LLI816_RS07050 (LLI816_07050)	-	1374583..1375146 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLI816_RS07055 (LLI816_07055)	-	1375319..1376497 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=956610 LLI816_RS07030 WP_010906131.1 1370979..1371626(-) (comEA) [Lactococcus lactis subsp. lactis strain I8-16]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=956610 LLI816_RS07030 WP_010906131.1 1370979..1371626(-) (comEA) [Lactococcus lactis subsp. lactis strain I8-16]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386