Detailed information NTG0956039

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: WG927_RS12845
• Genome accession: NZ_CP148896
• Coordinates: 2624290..2624904 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain XS142
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2619290..2629904

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WG927_RS12815 (WG927_12800)	rpsT	2619593..2619859 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
WG927_RS12820 (WG927_12805)	holA	2619822..2620917 (-)	1096	Protein_2478	DNA polymerase III subunit delta	-
WG927_RS12825 (WG927_12810)	-	2620957..2621108 (-)	152	Protein_2479	hypothetical protein	-
WG927_RS12830 (WG927_12815)	-	2621149..2621283 (+)	135	WP_003152870.1	YqzM family protein	-
WG927_RS12835 (WG927_12820)	comEC	2621302..2623653 (-)	2352	WP_029973403.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
WG927_RS12840 (WG927_12825)	-	2623654..2624223 (-)	570	WP_003152868.1	ComE operon protein 2	-
WG927_RS12845 (WG927_12830)	comEA	2624290..2624904 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
WG927_RS12850 (WG927_12835)	comER	2624963..2625784 (+)	822	WP_029973401.1	late competence protein ComER	-
WG927_RS12855 (WG927_12840)	-	2625853..2626590 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
WG927_RS12860 (WG927_12845)	rsfS	2626587..2626943 (-)	357	WP_007408260.1	ribosome silencing factor	-
WG927_RS12865 (WG927_12850)	yqeK	2626961..2627521 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
WG927_RS12870 (WG927_12855)	-	2627511..2628080 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
WG927_RS12875 (WG927_12860)	yhbY	2628091..2628381 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
WG927_RS12880 (WG927_12865)	aroE	2628375..2629217 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=956039 WG927_RS12845 WP_014418419.1 2624290..2624904(-) (comEA) [Bacillus velezensis strain XS142]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=956039 WG927_RS12845 WP_014418419.1 2624290..2624904(-) (comEA) [Bacillus velezensis strain XS142]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363