Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WCU20_RS24075 Genome accession   NZ_CP147541
Coordinates   5176470..5178170 (+) Length   566 a.a.
NCBI ID   WP_003112841.1    Uniprot ID   P22608
Organism   Pseudomonas aeruginosa strain GN06288     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5171470..5183170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU20_RS24050 (WCU20_24050) ampD 5171482..5172048 (-) 567 WP_003122083.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WCU20_RS24055 (WCU20_24055) - 5172199..5174454 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  WCU20_RS24060 (WCU20_24060) nadC 5174740..5175588 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WCU20_RS24070 (WCU20_24070) pilA 5175787..5176239 (-) 453 WP_028681203.1 pilin Machinery gene
  WCU20_RS24075 (WCU20_24075) pilB 5176470..5178170 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WCU20_RS24080 (WCU20_24080) pilC 5178174..5179394 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  WCU20_RS24085 (WCU20_24085) pilD 5179398..5180270 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  WCU20_RS24090 (WCU20_24090) coaE 5180267..5180878 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  WCU20_RS24095 (WCU20_24095) yacG 5180875..5181075 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  WCU20_RS24100 (WCU20_24100) - 5181112..5181321 (-) 210 WP_003094660.1 hypothetical protein -
  WCU20_RS24105 (WCU20_24105) - 5181427..5182116 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  WCU20_RS24110 (WCU20_24110) - 5182113..5182583 (-) 471 WP_003125205.1 hypothetical protein -
  WCU20_RS24115 (WCU20_24115) - 5182580..5183005 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62328.19 Da        Isoelectric Point: 5.7041

>NTDB_id=946839 WCU20_RS24075 WP_003112841.1 5176470..5178170(+) (pilB) [Pseudomonas aeruginosa strain GN06288]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQSKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=946839 WCU20_RS24075 WP_003112841.1 5176470..5178170(+) (pilB) [Pseudomonas aeruginosa strain GN06288]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACTTGGTGCAGAGCAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTTGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTCTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P22608

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.517

100

0.581
  pilB Acinetobacter baylyi ADP1

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

51.064

99.647

0.509
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio cholerae strain A1552

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.823

99.647

0.496
  pilF Thermus thermophilus HB27

39.75

99.117

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366