Detailed information NTG0945537

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: WAA17_RS13060
• Genome accession: NZ_CP147000
• Coordinates: 2671101..2671715 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis isolate K8
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2666101..2676715

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WAA17_RS13030 (WAA17_13030)	rpsT	2666401..2666667 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
WAA17_RS13035 (WAA17_13035)	holA	2666684..2667725 (-)	1042	Protein_2522	DNA polymerase III subunit delta	-
WAA17_RS13040 (WAA17_13040)	-	2667765..2667919 (-)	155	Protein_2523	hypothetical protein	-
WAA17_RS13045 (WAA17_13045)	-	2667960..2668094 (+)	135	WP_003152870.1	YqzM family protein	-
WAA17_RS13050 (WAA17_13050)	comEC	2668113..2670464 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
WAA17_RS13055 (WAA17_13055)	-	2670465..2671034 (-)	570	WP_003152868.1	ComE operon protein 2	-
WAA17_RS13060 (WAA17_13060)	comEA	2671101..2671715 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
WAA17_RS13065 (WAA17_13065)	comER	2671774..2672595 (+)	822	WP_014305449.1	late competence protein ComER	-
WAA17_RS13070 (WAA17_13070)	-	2672664..2673401 (-)	738	WP_024085626.1	class I SAM-dependent methyltransferase	-
WAA17_RS13075 (WAA17_13075)	rsfS	2673398..2673754 (-)	357	WP_003152864.1	ribosome silencing factor	-
WAA17_RS13080 (WAA17_13080)	yqeK	2673772..2674332 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
WAA17_RS13085 (WAA17_13085)	-	2674322..2674891 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
WAA17_RS13090 (WAA17_13090)	yhbY	2674902..2675192 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
WAA17_RS13095 (WAA17_13095)	aroE	2675186..2676028 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=945537 WAA17_RS13060 WP_041482377.1 2671101..2671715(-) (comEA) [Bacillus velezensis isolate K8]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=945537 WAA17_RS13060 WP_041482377.1 2671101..2671715(-) (comEA) [Bacillus velezensis isolate K8]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363