Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SGN16_RS10625 Genome accession   NZ_CP146985
Coordinates   2362069..2363184 (+) Length   371 a.a.
NCBI ID   WP_210643908.1    Uniprot ID   -
Organism   Pseudomonas sp. G166     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2357069..2368184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGN16_RS10610 - 2358040..2358954 (+) 915 WP_335943960.1 sugar kinase -
  SGN16_RS10615 - 2359077..2359352 (-) 276 WP_019690952.1 peptidylprolyl isomerase -
  SGN16_RS10620 - 2359398..2361935 (-) 2538 WP_335943961.1 PAS domain-containing protein -
  SGN16_RS10625 pilU 2362069..2363184 (+) 1116 WP_210643908.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SGN16_RS10630 - 2363337..2365292 (-) 1956 WP_335943962.1 acetoacetate--CoA ligase -
  SGN16_RS10635 - 2365451..2366227 (-) 777 WP_210643912.1 3-hydroxybutyrate dehydrogenase -
  SGN16_RS10640 - 2366235..2367626 (-) 1392 WP_210643914.1 GntP family permease -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41272.26 Da        Isoelectric Point: 6.0675

>NTDB_id=945341 SGN16_RS10625 WP_210643908.1 2362069..2363184(+) (pilU) [Pseudomonas sp. G166]
MEIDPLLRILASQDGSDLYMSTGAPPCARFEGVLKPLGNQAFKVGEIAGLAESLMDAEQRLEFDRELEMNLAISMAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDQAL
FNLVVEGAIDEEEALKNADSTNNLRLRLKLHAETGAVPPPDPAAGEWGLVD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=945341 SGN16_RS10625 WP_210643908.1 2362069..2363184(+) (pilU) [Pseudomonas sp. G166]
ATGGAAATCGATCCATTGTTGCGAATCCTGGCAAGCCAGGATGGCTCCGATCTTTACATGTCCACCGGCGCGCCGCCGTG
CGCGCGATTCGAGGGCGTGCTCAAGCCCCTGGGTAACCAGGCATTCAAGGTCGGCGAGATCGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAGTTGGAAATGAACCTGGCGATCTCCATGGCCGGTGTCGGGCGC
TTCCGGGTCAATATCTTCAAGCAGCGCAACGATGTGTCGATGGTGATACGCAACGTCAAGCTGGACATTCCACGTTTCGA
AGACCTGAAACTGCCACGGGTGCTGCTCGATACCATCATGCAGAAGCAAGGGCTGATGCTGTTCGTCGGGGCGACAGGGT
CAGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCATAAGAAATCAATCATCAACCAGCGGGAAGTCGGTGTCGACACCCGCAGTTTTCATGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAAATCCGTGATCGCGAAACCATGGAACATG
CCCTGGCCTTCGCCGACACCGGGCATCTGGTGATCTCCACGCTGCATGCCCACAACGCCAACCAGGCCCTGGACCGAGTG
ATCAATTTTTTCCCGGAAGAGCGCCGGCCACAGTTGCTTAATGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAACG
CCTGGTGCGCACCCGCACCGGCCAGCGTCGGGCGGCGGTGGAGGTCATGCTGGGCTCGCCCACGGTGGCCGACCTGATCC
GCCGCAATGAGCTGGGCGAGCTCAAGGGCATCATGGAAAAATCCGAAGAGTTGGGGATGCAAACTTTCGATCAGGCGTTG
TTCAACCTGGTGGTCGAGGGGGCGATCGATGAGGAAGAGGCGCTGAAAAACGCTGATTCAACGAACAACCTGCGCTTGCG
GCTGAAGTTGCATGCCGAGACGGGCGCGGTGCCGCCACCTGATCCGGCGGCGGGTGAGTGGGGGTTGGTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.901

95.687

0.544
  pilU Acinetobacter baylyi ADP1

52.113

95.687

0.499
  pilU Vibrio cholerae strain A1552

52.286

94.34

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.108

99.73

0.38
  pilT Pseudomonas stutzeri DSM 10701

39.244

92.722

0.364
  pilT Pseudomonas aeruginosa PAK

38.953

92.722

0.361