Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WAX86_RS12720 Genome accession   NZ_CP146970
Coordinates   2802031..2803137 (-) Length   368 a.a.
NCBI ID   WP_005297691.1    Uniprot ID   A0A2T3I981
Organism   Photobacterium damselae subsp. damselae strain WMD-P2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2797031..2808137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAX86_RS12690 (WAX86_12690) - 2797416..2797898 (+) 483 WP_341488872.1 SprT family zinc-dependent metalloprotease -
  WAX86_RS12695 (WAX86_12695) endA 2798029..2798733 (+) 705 WP_005297700.1 deoxyribonuclease I -
  WAX86_RS12700 (WAX86_12700) rsmE 2798865..2799596 (+) 732 WP_044174434.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WAX86_RS12705 (WAX86_12705) gshB 2799617..2800564 (+) 948 WP_341488873.1 glutathione synthase -
  WAX86_RS12710 (WAX86_12710) - 2800718..2801281 (+) 564 WP_341488874.1 YqgE/AlgH family protein -
  WAX86_RS12715 (WAX86_12715) ruvX 2801278..2801703 (+) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  WAX86_RS12720 (WAX86_12720) pilU 2802031..2803137 (-) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WAX86_RS12725 (WAX86_12725) pilT 2803149..2804183 (-) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  WAX86_RS12730 (WAX86_12730) - 2804206..2804907 (+) 702 WP_341488875.1 YggS family pyridoxal phosphate-dependent enzyme -
  WAX86_RS12735 (WAX86_12735) proC 2804923..2805741 (+) 819 WP_005297684.1 pyrroline-5-carboxylate reductase -
  WAX86_RS12740 (WAX86_12740) - 2805753..2806310 (+) 558 WP_005297682.1 YggT family protein -
  WAX86_RS12745 (WAX86_12745) yggU 2806307..2806606 (+) 300 WP_036763214.1 DUF167 family protein YggU -
  WAX86_RS12750 (WAX86_12750) - 2806708..2807301 (+) 594 WP_044174415.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41354.51 Da        Isoelectric Point: 5.5884

>NTDB_id=945216 WAX86_RS12720 WP_005297691.1 2802031..2803137(-) (pilU) [Photobacterium damselae subsp. damselae strain WMD-P2]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGVLHPIGDTLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=945216 WAX86_RS12720 WP_005297691.1 2802031..2803137(-) (pilU) [Photobacterium damselae subsp. damselae strain WMD-P2]
ATGAAACTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGTGTGTTGCATCCCATTGGCGATACGTTAGATCGTACTGGGGTTGATTTACTGTTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGGGGCGATTGGCGGTTTCGT
GTTAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGTGCAACAGGGTCGGGTA
AATCGACTTCGATGGCGGCAATGACCGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGTCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTGCGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCAGAAACCGGTCATTTATGTATGGCCACATTGCATGCCAATAATGCTAACCAAGCGTTAGAGCGCATTCTGCAT
CTGGTACCGAAAGAGCGGCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCTGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACTAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTTACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3I981

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

99.728

0.679
  pilU Pseudomonas stutzeri DSM 10701

59.497

97.283

0.579
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

93.75

0.389
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367