Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WAX87_RS13140 Genome accession   NZ_CP146967
Coordinates   2853586..2854692 (-) Length   368 a.a.
NCBI ID   WP_005297691.1    Uniprot ID   A0A2T3I981
Organism   Photobacterium damselae subsp. damselae strain WMD-P1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2848586..2859692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAX87_RS13110 (WAX87_13110) - 2848971..2849453 (+) 483 WP_036763215.1 SprT family zinc-dependent metalloprotease -
  WAX87_RS13115 (WAX87_13115) endA 2849584..2850288 (+) 705 WP_005297700.1 deoxyribonuclease I -
  WAX87_RS13120 (WAX87_13120) rsmE 2850420..2851151 (+) 732 WP_005297699.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WAX87_RS13125 (WAX87_13125) gshB 2851172..2852119 (+) 948 WP_005297697.1 glutathione synthase -
  WAX87_RS13130 (WAX87_13130) - 2852273..2852836 (+) 564 WP_068945809.1 YqgE/AlgH family protein -
  WAX87_RS13135 (WAX87_13135) ruvX 2852833..2853258 (+) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  WAX87_RS13140 (WAX87_13140) pilU 2853586..2854692 (-) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WAX87_RS13145 (WAX87_13145) pilT 2854704..2855738 (-) 1035 WP_005297689.1 type IV pilus twitching motility protein PilT Machinery gene
  WAX87_RS13150 (WAX87_13150) - 2855761..2856462 (+) 702 WP_005297686.1 YggS family pyridoxal phosphate-dependent enzyme -
  WAX87_RS13155 (WAX87_13155) proC 2856478..2857296 (+) 819 WP_005297684.1 pyrroline-5-carboxylate reductase -
  WAX87_RS13160 (WAX87_13160) - 2857308..2857865 (+) 558 WP_005297682.1 YggT family protein -
  WAX87_RS13165 (WAX87_13165) yggU 2857862..2858161 (+) 300 WP_036763214.1 DUF167 family protein YggU -
  WAX87_RS13170 (WAX87_13170) - 2858263..2858856 (+) 594 WP_106260358.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41354.51 Da        Isoelectric Point: 5.5884

>NTDB_id=945181 WAX87_RS13140 WP_005297691.1 2853586..2854692(-) (pilU) [Photobacterium damselae subsp. damselae strain WMD-P1]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGVLHPIGDTLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=945181 WAX87_RS13140 WP_005297691.1 2853586..2854692(-) (pilU) [Photobacterium damselae subsp. damselae strain WMD-P1]
ATGAAACTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGTGTGTTGCATCCCATTGGCGATACGTTAGATCGTACAGGGGTTGATTTACTGTTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGGGGCGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGTGCAACGGGGTCGGGTA
AATCGACTTCGATGGCGGCAATGACCGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGCCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTGCGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTTGCGGAAACGGGTCATTTATGTATGGCCACACTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGCATTCTGCAT
CTGGTACCGAAAGAGCGCCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCTGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACTAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTAACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3I981

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

99.728

0.679
  pilU Pseudomonas stutzeri DSM 10701

59.497

97.283

0.579
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

93.75

0.389
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367