Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   WA084_RS02605 Genome accession   NZ_CP146920
Coordinates   547266..547700 (+) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain 212516     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 542266..552700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WA084_RS02575 tadA 542376..542876 (-) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -
  WA084_RS02580 - 542891..543742 (-) 852 WP_014391366.1 thymidylate synthase -
  WA084_RS02585 lgt 543739..544551 (-) 813 WP_014391365.1 prolipoprotein diacylglyceryl transferase -
  WA084_RS02590 - 544561..545355 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  WA084_RS02595 rppH 545358..545954 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  WA084_RS02600 ampD 546540..547106 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WA084_RS02605 pilA 547266..547700 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  WA084_RS02610 pilB 547700..549085 (+) 1386 WP_338705846.1 GspE/PulE family protein Machinery gene
  WA084_RS02615 - 549085..550296 (+) 1212 WP_079157761.1 type II secretion system F family protein -
  WA084_RS02620 - 550296..551018 (+) 723 WP_041423102.1 A24 family peptidase -
  WA084_RS02625 coaE 551033..551653 (+) 621 WP_014391361.1 dephospho-CoA kinase -
  WA084_RS02630 yacG 551664..551867 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  WA084_RS02635 - 551867..552136 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=945084 WA084_RS02605 WP_005741096.1 547266..547700(+) (pilA) [Pasteurella multocida strain 212516]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=945084 WA084_RS02605 WP_005741096.1 547266..547700(+) (pilA) [Pasteurella multocida strain 212516]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTTACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368