Detailed information NTG0944916

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACFXAD_RS12785
• Genome accession: NZ_CP170653
• Coordinates: 2604843..2607254 (-)
• Length: 803 a.a.
• NCBI ID: WP_053573428.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JP5011
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2599843..2612254

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFXAD_RS12760 (ACFXAD_12760)	-	2600186..2600947 (-)	762	WP_007409750.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
ACFXAD_RS12765 (ACFXAD_12765)	rlmN	2600953..2602044 (-)	1092	WP_053573429.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
ACFXAD_RS12770 (ACFXAD_12770)	rsmB	2602048..2603391 (-)	1344	WP_014417745.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
ACFXAD_RS12775 (ACFXAD_12775)	fmt	2603378..2604331 (-)	954	WP_014417744.1	methionyl-tRNA formyltransferase	-
ACFXAD_RS12780 (ACFXAD_12780)	def	2604336..2604818 (-)	483	WP_014417743.1	peptide deformylase	-
ACFXAD_RS12785 (ACFXAD_12785)	priA	2604843..2607254 (-)	2412	WP_053573428.1	primosomal protein N'	Machinery gene
ACFXAD_RS12790 (ACFXAD_12790)	coaBC	2607251..2608471 (-)	1221	WP_053573427.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ACFXAD_RS12795 (ACFXAD_12795)	rpoZ	2608562..2608765 (-)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
ACFXAD_RS12800 (ACFXAD_12800)	gmk	2608769..2609383 (-)	615	WP_003154347.1	guanylate kinase	-
ACFXAD_RS12805 (ACFXAD_12805)	remA	2609391..2609660 (-)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
ACFXAD_RS12810 (ACFXAD_12810)	-	2609737..2610612 (-)	876	WP_053573462.1	YicC/YloC family endoribonuclease	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90819.85 Da
• Isoelectric Point: 8.8944

>NTDB_id=944916 ACFXAD_RS12785 WP_053573428.1 2604843..2607254(-) (priA) [Bacillus amyloliquefaciens strain JP5011]
MNVAEVIVDVSSKNIDRPFDYKIPDHLKGMIEVGMRVIVPFGPRKLQGFVTGLKDSSELNGGSMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIKQLFRNQKSLLYSDITDESILSSLQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQASKQLAVLTYFIGGGAKISAAELCKKTDSSSATLKTLLKKGL
LTESFEEVYRDPYQDRMFKKTEPLSLTEEQRAAFEPIKQAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGQEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSRELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQIQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=944916 ACFXAD_RS12785 WP_053573428.1 2604843..2607254(-) (priA) [Bacillus amyloliquefaciens strain JP5011]
ATGAATGTAGCAGAAGTCATTGTCGATGTCAGCTCCAAAAACATTGACAGACCCTTTGATTACAAAATTCCCGATCATTT
AAAAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTTGGCCCGCGCAAGCTTCAAGGATTTGTGACCGGTTTGA
AAGATTCGTCTGAATTAAACGGCGGCTCAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACGCCCGTTCTGACTGAGGAA
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATTACGGCGCTTCAGGCAATGCTGCCCGCCGC
ACTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAAACAGCTTTTCAGGA
ATCAAAAAAGTCTTCTGTACTCGGATATTACCGATGAAAGCATTCTTTCGTCACTTCAAAAATTCGTGCGCAAAGGAAGC
ATTGATGTCACATATAAAGTCGCCCAAAAAACGAATAAAAAAATGATCCGCATGATCGAAGCGGGGGCAGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAGCAGGCTTCTAAACAGCTCGCCGTGCTTACTTATTTTATCGGCGGGGGAG
CAAAGATTTCCGCGGCCGAGCTTTGCAAAAAGACCGATTCCAGTTCCGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGAAAGCTTTGAGGAGGTTTACCGCGATCCCTATCAGGACAGAATGTTCAAAAAAACAGAGCCGCTCTCTCTTAC
AGAAGAGCAGCGGGCTGCTTTTGAGCCGATTAAACAAGCCGTAGCCGACCATGTCCATCACGTCTTTCTGCTTCACGGCG
TCACTGGAAGCGGCAAAACGGAAATTTACCTGCAATCCATTGAAAAAGTGCTGGCAAAAGGACAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCGTTGACACCGCAAATGGTCAACCGTTTTAAAGGCCGCTTCGGCTCACAGGTTGCGGTCATGCACAG
CGGATTATCCACAGGTGAAAAATACGATGAATGGCGGAAGATTCAGCGCAAGGAAGTCCGGCTTGTTGTCGGAGCCAGAT
CGGCCATCTTTGCCCCGTTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCTTCCTATAAGCAGGAGGAA
ATGCCGCGGTATCATGCAAAGGACGTAGCCATCCGCCGGGCCGAGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAATCATTTGCGCGGGCGAAAAAAGGCGTTTATGAGCTGCTACCGTTAAAACATCGCGTAAATCGCCAGC
TCATGCCTGACGTTTCTCTCGTTGATATGAGAGAAGAACTGAGAAACGGCAACAGATCAATGTTTTCAAGAGAGCTGATG
GAACAGCTGGAAGAGAAGCTTGCAAAAAAAGAGCAGGCTGTCCTTTTCCTGAATAAACGGGGGTATTCCTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCGCAATGTCCGCATTGCGACATTTCAATGACTTATCACCGCTACGGACAAAGGCTGA
AATGCCATTACTGCGGCCATGAGGAACCTGTCCCGGGAACATGCCCGGAATGCGGCAGCGAACATATCCGCTTTTTCGGC
ACAGGCACGCAGCGTGTGGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACCAC
TTCAAGAAAAGGCGCCCATGAAAAACTGTTGTCAGCGTTCGGTGAAGGGAAGGCGGATATCCTGCTCGGTACGCAAATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCTGATTTC
AGGGCTGCGGAGAAGACATTCCAGCTGCTGACGCAGGTAAGCGGACGGGCCGGGCGGCATGAAAAGCCGGGAACGGTGGT
CATTCAAACGTATACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCACGACTATGAAACTTTTTTTCAGCAGGAAA
TGGCGCACAGACGGGCCCAGTCATACCCGCCTTATTATTACGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCGGCAAAA
GCCGCGGTCACCGCAGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGGCCTGATACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATCGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGATTC
AGTTGAAAAAGATACTGGAGCACTATAAACGCGACATTGCGCAAAAGCATGTAATGATTTCAATCGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	84.472	100	0.847