Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WAC51_RS16100 Genome accession   NZ_CP146845
Coordinates   3510001..3511728 (-) Length   575 a.a.
NCBI ID   WP_338712877.1    Uniprot ID   -
Organism   Stenotrophomonas geniculata strain WXY53     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3505001..3516728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAC51_RS16085 (WAC51_16085) sucC 3505248..3506417 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  WAC51_RS16090 (WAC51_16090) - 3506823..3508436 (+) 1614 WP_338712876.1 ATP-binding protein -
  WAC51_RS16095 (WAC51_16095) pilR 3508497..3509870 (+) 1374 WP_217014088.1 sigma-54 dependent transcriptional regulator Regulator
  WAC51_RS16100 (WAC51_16100) pilB 3510001..3511728 (-) 1728 WP_338712877.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WAC51_RS16105 (WAC51_16105) - 3511742..3514798 (-) 3057 WP_338712878.1 fused MFS/spermidine synthase -
  WAC51_RS16110 (WAC51_16110) - 3514903..3515589 (-) 687 WP_338712879.1 serine hydrolase domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62264.20 Da        Isoelectric Point: 6.1552

>NTDB_id=944666 WAC51_RS16100 WP_338712877.1 3510001..3511728(-) (pilB) [Stenotrophomonas geniculata strain WXY53]
MSVVSPPNLAGITGLARRLVQDGALDEASARDAMAKATAARQPLPTWFAQNKLVSASQLAAANAVEFGMPLFDVSTFDTS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHALDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTIGDALGGD
DEGMANLDVGLGDEDNAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFH
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPANLPEHA
LLAEGFTQAQLDAGIQLYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=944666 WAC51_RS16100 WP_338712877.1 3510001..3511728(-) (pilB) [Stenotrophomonas geniculata strain WXY53]
ATGAGCGTTGTTTCCCCTCCCAACCTCGCCGGCATCACCGGCCTGGCCCGCCGTCTGGTCCAGGACGGCGCCTTGGACGA
GGCATCCGCCCGCGACGCCATGGCCAAGGCCACGGCGGCCCGCCAGCCGCTGCCCACGTGGTTCGCGCAGAACAAGCTGG
TCAGTGCTTCGCAGCTCGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCCCTATTCGACGTGTCCACCTTCGATACCAGC
CAGAACGCGATGAGCTTGGTCAGCGAGGAGCTGCTGCGCAAGCACAATGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAACCCGACCCATGCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAACCGATCCTGG
TCGATGAAGACCAGATCCGCCGCACGTTGGAGCAGTGGCACGCCAGCCACGACACCATCGGCGATGCGCTGGGCGGCGAC
GACGAGGGCATGGCCAACCTCGACGTTGGCCTGGGCGACGAAGACAACGCGGGTGGCGACTCCGGCATCGACGCCAAGGG
TGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTACTGGTCGATGCGATCCGCAAGGGCGCGTCGGACATCCACTTCG
AACCCTACGAAGACGATTACCGTGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCGGTGAAGTTG
AACCAGCGCATTGCCGCGCGTTTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGACGCAT
CAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGTGAAAAGGTGGTGC
TGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTTGGCTACGAACCAGACCAGCAGAAGCTGTTCCAC
GACGCCATCCACAAGCCGTATGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACCGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACTGCCGAAGACCCGGTGGAAATCCGCTTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCTGCGGCGCTGCGCTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTCCAC
GCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCCCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATCGCGCAGCGTCTGGCACGCCGGTTGTGCAGCAACTGCAAGCGTCCGGCCAACCTGCCGGAGCATGCG
TTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCCAGTTGTACGAGGCGGTGGGCTGCGATGAGTGCAC
CGAGGGCTACAAGGGCCGAACCGGTATCTACCAGGTGATGCCGATGACCGATGAGATCGCCACCATCGTGCTGGCCGGTG
GCAATGCACTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCC
GCGGGTGTGACCAGCCTGGCCGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.143

98.609

0.563
  pilB Acinetobacter baylyi ADP1

55.979

100

0.562
  pilB Legionella pneumophila strain ERS1305867

51.313

99.304

0.51
  pilF Neisseria gonorrhoeae MS11

48.944

98.783

0.483
  pilB Vibrio cholerae strain A1552

48.601

99.478

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

45.884

99.304

0.456
  pilB Vibrio campbellii strain DS40M4

44.991

98.957

0.445
  pilF Thermus thermophilus HB27

37.589

98.087

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.645

94.957

0.367