Detailed information NTG0944519

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: V9W62_RS12630
• Genome accession: NZ_CP146763
• Coordinates: 2655886..2656500 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain AP81
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2650886..2661500

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V9W62_RS12600 (V9W62_12600)	rpsT	2651189..2651455 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
V9W62_RS12605 (V9W62_12605)	holA	2651472..2652513 (-)	1042	Protein_2436	DNA polymerase III subunit delta	-
V9W62_RS12610 (V9W62_12610)	-	2652553..2652704 (-)	152	Protein_2437	hypothetical protein	-
V9W62_RS12615 (V9W62_12615)	-	2652745..2652879 (+)	135	WP_003152870.1	YqzM family protein	-
V9W62_RS12620 (V9W62_12620)	comEC	2652898..2655249 (-)	2352	WP_043021636.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
V9W62_RS12625 (V9W62_12625)	-	2655250..2655819 (-)	570	WP_003152868.1	ComE operon protein 2	-
V9W62_RS12630 (V9W62_12630)	comEA	2655886..2656500 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
V9W62_RS12635 (V9W62_12635)	comER	2656559..2657380 (+)	822	WP_043021638.1	late competence protein ComER	-
V9W62_RS12640 (V9W62_12640)	-	2657449..2658186 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
V9W62_RS12645 (V9W62_12645)	rsfS	2658183..2658539 (-)	357	WP_007408260.1	ribosome silencing factor	-
V9W62_RS12650 (V9W62_12650)	yqeK	2658557..2659117 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
V9W62_RS12655 (V9W62_12655)	-	2659107..2659676 (-)	570	WP_043021639.1	nicotinate-nucleotide adenylyltransferase	-
V9W62_RS12660 (V9W62_12660)	yhbY	2659687..2659977 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
V9W62_RS12665 (V9W62_12665)	aroE	2659971..2660813 (-)	843	WP_043021640.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=944519 V9W62_RS12630 WP_043021637.1 2655886..2656500(-) (comEA) [Bacillus velezensis strain AP81]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=944519 V9W62_RS12630 WP_043021637.1 2655886..2656500(-) (comEA) [Bacillus velezensis strain AP81]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363