Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   V9W60_RS16605 Genome accession   NZ_CP146759
Coordinates   3214346..3215473 (-) Length   375 a.a.
NCBI ID   WP_041091472.1    Uniprot ID   -
Organism   Bacillus altitudinis strain JJ61     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3209346..3220473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9W60_RS16570 (V9W60_16570) flgL 3209529..3210440 (-) 912 WP_008348257.1 flagellar hook-associated protein FlgL -
  V9W60_RS16575 (V9W60_16575) flgK 3210449..3211969 (-) 1521 WP_024719239.1 flagellar hook-associated protein FlgK -
  V9W60_RS16580 (V9W60_16580) - 3211985..3212467 (-) 483 WP_007499977.1 flagellar protein FlgN -
  V9W60_RS16585 (V9W60_16585) flgM 3212486..3212749 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  V9W60_RS16590 (V9W60_16590) - 3212829..3213248 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  V9W60_RS16595 (V9W60_16595) comFC 3213305..3213991 (-) 687 WP_198527543.1 ComF family protein Machinery gene
  V9W60_RS16600 (V9W60_16600) - 3213988..3214281 (-) 294 WP_008348267.1 competence protein ComFB -
  V9W60_RS16605 (V9W60_16605) comFA 3214346..3215473 (-) 1128 WP_041091472.1 DEAD/DEAH box helicase Machinery gene
  V9W60_RS16610 (V9W60_16610) - 3215845..3216687 (-) 843 WP_024719242.1 DegV family protein -
  V9W60_RS16615 (V9W60_16615) degU 3216899..3217588 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  V9W60_RS16620 (V9W60_16620) degS 3217653..3218828 (-) 1176 WP_024719243.1 sensor histidine kinase Regulator
  V9W60_RS16625 (V9W60_16625) - 3219050..3219718 (+) 669 WP_335364162.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42926.95 Da        Isoelectric Point: 10.4727

>NTDB_id=944263 V9W60_RS16605 WP_041091472.1 3214346..3215473(-) (comFA) [Bacillus altitudinis strain JJ61]
MLGKVSECGFLYEWTGPQKKETRRVELTWQGELSKGQKRASDRLVEAIQNKFDLLIWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSIDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVAHRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQVQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
SESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=944263 V9W60_RS16605 WP_041091472.1 3214346..3215473(-) (comFA) [Bacillus altitudinis strain JJ61]
ATGCTGGGAAAAGTAAGCGAATGTGGCTTTCTCTACGAATGGACGGGTCCACAAAAGAAAGAAACAAGACGAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCATTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCATGGAATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTTGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCGCCGCTTATGATCGCTACAACCCATCAGCTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTTGACGAAGTCGATGCATTTCCTTATTCAATTGATGAACGGCTTCAGTTT
GCTGTTTTAAAATCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAGGATGT
TGCTCATAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGTTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGATACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAGGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
TCAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTGTATTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.946

98.667

0.552
  comFA/cflA Streptococcus pneumoniae Rx1

40.395

94.4

0.381
  comFA/cflA Streptococcus pneumoniae D39

40.395

94.4

0.381
  comFA/cflA Streptococcus pneumoniae R6

40.395

94.4

0.381
  comFA Latilactobacillus sakei subsp. sakei 23K

38.587

98.133

0.379
  comFA/cflA Streptococcus pneumoniae TIGR4

40.113

94.4

0.379
  comFA/cflA Streptococcus mitis NCTC 12261

39.831

94.4

0.376
  comFA/cflA Streptococcus mitis SK321

38.701

94.4

0.365
  comFA Lactococcus lactis subsp. cremoris KW2

37.158

97.6

0.363