Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   V4331_RS00665 Genome accession   NZ_CP146743
Coordinates   138008..139027 (+) Length   339 a.a.
NCBI ID   WP_341784489.1    Uniprot ID   -
Organism   Lactococcus formosensis subsp. formosensis strain L5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 133008..144027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4331_RS00645 (V4331_00645) guaB 133136..134617 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  V4331_RS00650 (V4331_00650) - 134973..136634 (+) 1662 WP_341784218.1 S-layer homology domain-containing protein -
  V4331_RS00655 (V4331_00655) rpsU 136763..136939 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  V4331_RS00660 (V4331_00660) comGA 137125..138069 (+) 945 WP_341784488.1 competence type IV pilus ATPase ComGA Machinery gene
  V4331_RS00665 (V4331_00665) comGB 138008..139027 (+) 1020 WP_341784489.1 competence type IV pilus assembly protein ComGB Machinery gene
  V4331_RS00670 (V4331_00670) comYC 139046..139351 (+) 306 WP_096368800.1 competence type IV pilus major pilin ComGC Machinery gene
  V4331_RS00675 (V4331_00675) comGD 139326..139742 (+) 417 Protein_124 competence type IV pilus minor pilin ComGD -
  V4331_RS00680 (V4331_00680) comGE 139774..140007 (+) 234 WP_269457349.1 competence type IV pilus minor pilin ComGE -
  V4331_RS00685 (V4331_00685) comGF 139994..140428 (+) 435 WP_231951074.1 competence type IV pilus minor pilin ComGF -
  V4331_RS00690 (V4331_00690) - 140663..141508 (+) 846 WP_096368796.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  V4331_RS00695 (V4331_00695) - 141595..142464 (-) 870 WP_165713363.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39405.12 Da        Isoelectric Point: 9.5240

>NTDB_id=943991 V4331_RS00665 WP_341784489.1 138008..139027(+) (comGB) [Lactococcus formosensis subsp. formosensis strain L5]
MDLSQLLQLRVKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLKFYEDI
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRRKLIQVSTYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYAIQNLPTI
FLGCLFGLLVFFYLCRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKK
FGVEFLQILDSGQKFEDALRFYPIFTKELALIVEYGELKAKLGQELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=943991 V4331_RS00665 WP_341784489.1 138008..139027(+) (comGB) [Lactococcus formosensis subsp. formosensis strain L5]
ATGGATTTATCTCAGCTGCTACAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAAACTTATTCAACTCATGAA
TAATCTTTTCAAAAGTGGTTTCCACCTCTCTGAAATTATTGATTTTCTTGAACGGTCGGGATTAACGGAGGCATATTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAATGGACAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTTATGAGGATATT
GTGACTCAGCTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACTTTGGGGCTGATTGAAGAAAAACTGATACGCGT
TTTGAACATCCGCAGAAAGCTCATCCAAGTCTCCACTTACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GCTTGAAAAATTATTTACTGCCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGCTATACAAAATCTTCCGACCATC
TTTTTAGGGTGCCTCTTCGGTTTGCTTGTTTTCTTTTACCTGTGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTGATGGTTAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACGGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTTGACTTACGTCAGATTTGTTTCATTATGCAAGAACAAAAAAGTCGAATCTTCAAAAAA
TTTGGTGTTGAATTCCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATGCCCTTCGATTTTACCCCATATTTACCAA
GGAGCTGGCATTAATTGTGGAATATGGTGAACTAAAGGCTAAGCTAGGGCAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTCGAAAGAGCAATGCAACTTATCCAGCCTATTGTTTTCTTGCTGGTGGCTTTGTTGATT
ATATTAATTTATGCTGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

52.959

99.705

0.528
  comGB/cglB Streptococcus mitis NCTC 12261

52.239

98.82

0.516
  comGB/cglB Streptococcus mitis SK321

51.642

98.82

0.51
  comGB/cglB Streptococcus pneumoniae D39

51.642

98.82

0.51
  comGB/cglB Streptococcus pneumoniae R6

51.642

98.82

0.51
  comGB/cglB Streptococcus pneumoniae TIGR4

51.642

98.82

0.51
  comGB/cglB Streptococcus pneumoniae Rx1

51.642

98.82

0.51
  comYB Streptococcus gordonii str. Challis substr. CH1

51.198

98.525

0.504
  comYB Streptococcus mutans UA140

50.476

92.92

0.469
  comYB Streptococcus mutans UA159

50.159

92.92

0.466