Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   V6Z91_RS13110 Genome accession   NZ_CP146614
Coordinates   2952395..2954080 (-) Length   561 a.a.
NCBI ID   WP_338770927.1    Uniprot ID   -
Organism   Massilia sp. METH4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2947395..2959080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6Z91_RS13095 - 2948795..2949751 (+) 957 WP_338770922.1 PfkB family carbohydrate kinase -
  V6Z91_RS13100 - 2949794..2951134 (+) 1341 WP_338770923.1 MFS transporter -
  V6Z91_RS13105 pilC 2951142..2952371 (-) 1230 WP_338770925.1 type II secretion system F family protein Machinery gene
  V6Z91_RS13110 pilF 2952395..2954080 (-) 1686 WP_338770927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V6Z91_RS13115 - 2954362..2955216 (+) 855 WP_338770928.1 helix-turn-helix domain-containing protein -
  V6Z91_RS13120 - 2955297..2955791 (+) 495 WP_338770930.1 NADH-quinone oxidoreductase subunit B family protein -
  V6Z91_RS13125 - 2955903..2957117 (+) 1215 WP_338770932.1 glutamate carboxypeptidase -
  V6Z91_RS13130 - 2957137..2958669 (+) 1533 WP_338770934.1 carboxylesterase family protein -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 60336.05 Da        Isoelectric Point: 6.3240

>NTDB_id=943404 V6Z91_RS13110 WP_338770927.1 2952395..2954080(-) (pilF) [Massilia sp. METH4]
MPGLARALVQAGRLTAAQADALVKRSAADKQPFIDVLLAGGAIGARDLAAFCSETFAYPMMDLHALSVAALPEKIIDEKL
MQAQRVVALAKRGNKMSVAISDPTNMQALDQIKFQTESLVEPVIVAHDALLRLLHELGKSSAQLIDELAGAEGSIAFAEE
QEAAAAEAPSTDIEDAPIVRFLNKMLMDAVNMGASDLHFEPFEKFYRIRFRVDGVLVEHAQPPVAIKDKLVSRIKVLARL
DISEKRVPQDGRMRLIVSPTRTLDLRVSTLPTLFGEKTVMRILDATQAQMGIDALGYEPEQKALMLDAIARPYGMVLVTG
PTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFPVALKSFLRQDPDIIMVGEIRDLETADIA
IKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCGCKKPLDIAPDKLVSAGFKPEELDGSW
KSYGPVGCERCNGSGYKGRVGIYQIMPITPAIEALILAHGNSMQIAAQAQAEGVKSLRQSGLVKVKAGLTSLEEVLGCTN
E

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=943404 V6Z91_RS13110 WP_338770927.1 2952395..2954080(-) (pilF) [Massilia sp. METH4]
ATGCCGGGATTGGCGCGGGCGCTGGTGCAGGCCGGTCGCCTGACGGCCGCCCAGGCGGACGCGCTGGTGAAACGCTCGGC
CGCCGACAAGCAGCCGTTCATCGACGTGCTGCTGGCCGGCGGCGCCATTGGCGCGCGCGACCTCGCCGCCTTCTGCTCCG
AGACCTTCGCCTACCCGATGATGGACTTGCACGCCCTCAGCGTTGCGGCCCTGCCCGAGAAGATCATCGATGAAAAGCTG
ATGCAGGCGCAGCGCGTGGTGGCGCTGGCCAAGCGGGGCAACAAGATGTCGGTGGCGATTTCCGATCCCACCAACATGCA
GGCGCTGGACCAGATCAAGTTCCAGACCGAGTCGCTGGTCGAACCCGTGATCGTGGCGCACGATGCGCTGCTGCGGCTGC
TGCACGAGCTCGGCAAGAGCAGCGCCCAGCTGATCGACGAGCTGGCCGGCGCCGAAGGTTCCATCGCCTTTGCCGAAGAG
CAGGAAGCGGCGGCCGCCGAAGCGCCGTCCACCGATATCGAGGATGCGCCCATCGTGCGCTTCCTGAACAAGATGCTGAT
GGATGCCGTGAACATGGGCGCCTCGGACCTGCACTTCGAACCGTTCGAGAAGTTCTACAGGATCAGGTTCCGGGTCGATG
GCGTGCTGGTCGAGCACGCGCAGCCGCCGGTGGCCATCAAGGACAAGCTGGTCTCGCGCATCAAGGTACTGGCCCGGCTC
GACATCTCCGAAAAGCGCGTGCCGCAGGATGGCCGCATGCGGTTGATCGTGTCGCCGACCCGGACGCTGGACCTGCGTGT
CTCCACGCTGCCCACGCTGTTCGGCGAAAAGACCGTCATGCGTATCCTCGACGCGACGCAGGCGCAGATGGGCATCGACG
CGCTGGGCTACGAGCCGGAACAGAAGGCGCTGATGCTGGACGCGATTGCCCGGCCGTACGGCATGGTGCTCGTCACCGGT
CCCACGGGTTCCGGCAAGACCGTGTCGCTGTACACCTGCCTGAACATCCTGAACAAGCCGGGCATCAATATCTCGACGGC
CGAAGACCCGGCCGAGATCAACCTGCCCGGCGTGAACCAGGTGAACGTGAACGACAAGGCGGGACTGACCTTCCCCGTCG
CGCTGAAGTCCTTCCTGCGCCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAAACGGCCGATATCGCG
ATCAAGGCGGCGCAGACGGGCCACATGGTGTTCTCCACGCTGCACACGAACGACGCGCCCTCGACGCTGACACGCCTGAT
GAACATGGGCGTGGCGCCGTTCAACATCGCCTCGTCCGTGATCCTGATCACGGCCCAGCGCCTGGCGCGGCGCCTGTGCG
GCTGCAAGAAGCCGCTCGATATCGCGCCGGACAAGCTGGTGAGCGCGGGCTTCAAGCCAGAGGAGCTCGATGGCAGCTGG
AAGTCGTACGGCCCGGTCGGCTGCGAACGCTGCAACGGCTCCGGCTACAAGGGGCGCGTGGGCATCTACCAGATCATGCC
GATCACGCCGGCGATCGAGGCGCTGATCCTCGCGCACGGCAACTCGATGCAGATCGCCGCCCAGGCCCAGGCCGAGGGCG
TGAAATCGCTGCGCCAGTCGGGCCTCGTGAAGGTGAAGGCCGGCCTGACGAGCCTCGAGGAAGTGCTGGGCTGCACGAAC
GAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-451)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.71

100

0.542
  pilB Acinetobacter baumannii D1279779

53.641

100

0.538
  pilB Acinetobacter baylyi ADP1

53.119

100

0.531
  pilB Legionella pneumophila strain ERS1305867

48.399

100

0.485
  pilB Vibrio cholerae strain A1552

46.924

100

0.476
  pilB Vibrio campbellii strain DS40M4

45.133

100

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

44.76

100

0.449
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.83

100

0.39
  pilF Thermus thermophilus HB27

38.505

95.365

0.367