Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V7R83_RS11350 Genome accession   NZ_CP146288
Coordinates   2759828..2761519 (+) Length   563 a.a.
NCBI ID   WP_267878749.1    Uniprot ID   -
Organism   Lautropia mirabilis ATCC 51599     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2754828..2766519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V7R83_RS11335 (V7R83_11335) - 2755216..2756601 (-) 1386 WP_040531662.1 NarK family nitrate/nitrite MFS transporter -
  V7R83_RS11340 (V7R83_11340) - 2756652..2757950 (-) 1299 WP_005674120.1 nitrate/nitrite transporter -
  V7R83_RS11345 (V7R83_11345) - 2758575..2759543 (+) 969 WP_040531668.1 Dyp-type peroxidase -
  V7R83_RS11350 (V7R83_11350) pilB 2759828..2761519 (+) 1692 WP_267878749.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V7R83_RS11355 (V7R83_11355) pilC 2761615..2762847 (+) 1233 WP_040531671.1 type II secretion system F family protein Machinery gene
  V7R83_RS11360 (V7R83_11360) pilD 2763059..2763976 (+) 918 WP_005674126.1 A24 family peptidase Machinery gene
  V7R83_RS11365 (V7R83_11365) coaE 2764155..2764817 (+) 663 WP_040531674.1 dephospho-CoA kinase -
  V7R83_RS11370 (V7R83_11370) zapD 2765013..2765768 (+) 756 WP_005674128.1 cell division protein ZapD -
  V7R83_RS11375 (V7R83_11375) - 2765768..2765989 (+) 222 WP_005674129.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61624.93 Da        Isoelectric Point: 4.9211

>NTDB_id=942562 V7R83_RS11350 WP_267878749.1 2759828..2761519(+) (pilB) [Lautropia mirabilis ATCC 51599]
MPGIARVLIQHQLLSPEQAREAHAKAQAAENGNFLDELVINSKVLNPLRLATFLAETFGMPLMDLASLDFSTLPLDQIDR
KLLAETRVIPLIKRGNRLAVLLSDPTDNQAIERVKFQQQAIIDPIVVEHSKLVKLIQNLDQSTADKLADLIGDVEEEESA
VADGAVALEDNADVDDAPIVRFLQKVLMDAIQGGASDIHFEPYEKFYRIRFRVDGELREITQPPLVIKDKLASRIKVISR
LDISEKRVPQDGRMKLVISNNRAIDFRVSTLPTLFGEKIVMRILDPSSAKLGIDALGYDPEQKKALTEAIARPYGMVLVT
GPTGSGKTVSLYTCLNILNQPGVNIATAEDPAEINLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIMVGEIRDLETADI
AIKAAQTGHMVMSTLHTNDAPTTLTRLMNMGVAPFNIASSVILITAQRLARRLCTQCKQPVDLDDDALLAAGFTEDDLDG
SWRPYKAVGCERCNGSGYKGRVGIYQVMPISEEIQRIILNSGNAMEIAAQAKREGVNDLRTSGLIKVKQGLTTIEEVLGV
TNE

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=942562 V7R83_RS11350 WP_267878749.1 2759828..2761519(+) (pilB) [Lautropia mirabilis ATCC 51599]
CTGCCCGGCATTGCCCGCGTGCTGATCCAGCACCAGCTGCTGTCGCCCGAGCAGGCGCGGGAAGCCCATGCCAAGGCTCA
GGCAGCCGAGAACGGCAACTTCCTCGACGAGCTGGTCATCAACAGCAAGGTGCTGAACCCGCTGCGGCTGGCCACCTTCC
TGGCCGAGACCTTCGGCATGCCGCTGATGGATCTGGCCAGCCTGGACTTCAGCACCCTGCCCCTGGACCAGATCGACCGC
AAGCTGCTGGCCGAGACCCGCGTCATCCCGCTGATCAAGCGCGGCAACCGCCTGGCCGTGCTGCTGTCCGACCCGACTGA
CAACCAGGCCATCGAGCGCGTCAAGTTCCAGCAGCAGGCCATCATCGACCCCATCGTCGTCGAGCACTCCAAGCTGGTCA
AGCTGATCCAGAACCTGGACCAGAGCACCGCCGACAAGCTGGCCGACCTGATCGGTGACGTGGAAGAAGAAGAGTCCGCT
GTCGCCGATGGCGCCGTGGCGCTGGAAGACAACGCCGACGTGGACGACGCGCCGATCGTGCGCTTCCTGCAGAAGGTGCT
GATGGATGCGATCCAGGGCGGTGCCTCGGACATCCACTTCGAGCCCTACGAGAAGTTCTACCGGATCCGCTTCCGGGTGG
ACGGCGAGCTGCGTGAGATCACGCAGCCGCCCCTGGTCATCAAGGACAAGCTCGCCTCGCGCATCAAGGTCATCTCCCGG
CTGGACATCTCGGAGAAGCGGGTGCCGCAGGACGGCCGGATGAAGCTGGTCATCTCCAACAACCGCGCCATCGACTTCCG
GGTCTCCACGCTGCCCACGCTGTTCGGCGAGAAGATCGTGATGCGTATTCTGGACCCCTCTTCCGCGAAGCTGGGGATCG
ATGCGCTGGGCTATGACCCCGAGCAGAAGAAGGCGCTGACCGAGGCCATCGCCCGTCCCTACGGCATGGTGCTGGTCACC
GGCCCCACCGGTTCGGGCAAGACCGTGTCGCTCTACACCTGCCTGAACATCCTGAACCAGCCGGGCGTCAACATCGCCAC
CGCCGAGGACCCGGCCGAGATCAACCTGCCCGGCATCAACCAGGTCAACGTCAACGACAAGGCAGGCCTGACCTTCGCTG
CAGCCCTGAAATCCTTCCTGCGTCAGGATCCGGACATCATCATGGTGGGTGAGATCCGCGACCTGGAAACTGCCGACATC
GCCATCAAGGCCGCCCAGACCGGCCACATGGTGATGTCCACGCTGCACACCAACGACGCCCCCACCACGCTCACCCGTCT
GATGAACATGGGCGTGGCACCCTTCAACATCGCCTCGTCGGTGATCCTGATCACCGCGCAGCGTCTGGCCCGTCGGCTGT
GCACCCAGTGCAAGCAGCCCGTGGACCTGGACGATGACGCCCTGCTGGCCGCCGGCTTCACCGAAGACGACCTGGACGGC
AGCTGGCGCCCCTACAAGGCCGTGGGCTGCGAGCGCTGCAACGGCTCGGGCTACAAGGGCCGGGTGGGCATCTACCAGGT
CATGCCGATCTCCGAAGAGATCCAGCGCATCATCCTCAATTCCGGCAACGCCATGGAAATTGCCGCCCAGGCCAAGCGCG
AGGGCGTCAACGACCTGCGCACCTCGGGGCTGATCAAGGTCAAGCAAGGCCTGACCACCATCGAGGAAGTGCTGGGCGTC
ACCAACGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(185-452)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.637

100

0.568
  pilF Neisseria gonorrhoeae MS11

55.95

100

0.56
  pilB Acinetobacter baylyi ADP1

54.867

100

0.551
  pilB Legionella pneumophila strain ERS1305867

52.289

100

0.528
  pilB Vibrio cholerae strain A1552

47.059

99.645

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

45.341

99.112

0.449
  pilB Vibrio campbellii strain DS40M4

44.207

99.645

0.44
  pilF Thermus thermophilus HB27

39.547

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.292

100

0.394