Detailed information    

insolico Bioinformatically predicted

Overview


Name   comYF   Type   Machinery gene
Locus tag   V6U62_RS00785 Genome accession   NZ_CP145860
Coordinates   150639..151076 (+) Length   145 a.a.
NCBI ID   WP_227071977.1    Uniprot ID   -
Organism   Streptococcus salivarius strain KSS2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 145639..156076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6U62_RS00755 (V6U62_00750) - 147312..147674 (+) 363 WP_002887019.1 DUF1033 family protein -
  V6U62_RS00760 (V6U62_00755) comYA 147756..148697 (+) 942 WP_002885658.1 competence type IV pilus ATPase ComGA Machinery gene
  V6U62_RS00765 (V6U62_00760) comYB 148579..149679 (+) 1101 WP_144429890.1 competence type IV pilus assembly protein ComGB Machinery gene
  V6U62_RS00770 (V6U62_00765) comYC 149676..150002 (+) 327 WP_002885815.1 competence type IV pilus major pilin ComGC Machinery gene
  V6U62_RS00775 (V6U62_00770) comYD 149962..150390 (+) 429 WP_253183813.1 competence type IV pilus minor pilin ComGD Machinery gene
  V6U62_RS00780 (V6U62_00775) comGE 150422..150652 (+) 231 WP_021144685.1 competence type IV pilus minor pilin ComGE -
  V6U62_RS00785 (V6U62_00780) comYF 150639..151076 (+) 438 WP_227071977.1 competence type IV pilus minor pilin ComGF Machinery gene
  V6U62_RS00790 (V6U62_00785) comGG 151054..151371 (+) 318 WP_227071976.1 competence type IV pilus minor pilin ComGG -
  V6U62_RS00795 (V6U62_00790) comYH 151416..152372 (+) 957 WP_227071975.1 class I SAM-dependent methyltransferase Machinery gene
  V6U62_RS00800 (V6U62_00795) - 152429..153622 (+) 1194 WP_410535066.1 acetate kinase -
  V6U62_RS00805 (V6U62_00800) - 153873..154070 (+) 198 WP_002885716.1 helix-turn-helix transcriptional regulator -
  V6U62_RS00810 (V6U62_00805) - 154082..154738 (+) 657 WP_410535064.1 CPBP family intramembrane glutamic endopeptidase -
  V6U62_RS00815 (V6U62_00810) - 154816..155262 (+) 447 WP_021144682.1 hypothetical protein -
  V6U62_RS00820 (V6U62_00815) - 155375..156037 (+) 663 WP_021144681.1 CPBP family intramembrane glutamic endopeptidase -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16169.60 Da        Isoelectric Point: 8.4658

>NTDB_id=940288 V6U62_RS00785 WP_227071977.1 150639..151076(+) (comYF) [Streptococcus salivarius strain KSS2]
MSLNSKVRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVKQQESQEWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKASTISQNNQIVTIQVTLKNGLQRSFVYAFEKTG

Nucleotide


Download         Length: 438 bp        

>NTDB_id=940288 V6U62_RS00785 WP_227071977.1 150639..151076(+) (comYF) [Streptococcus salivarius strain KSS2]
ATGTCTCTCAATAGCAAGGTTCGTGCTTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTGATTGCTGGTTCAGTTCA
AGTTTATCAAGGTCTGACCACAGTATTGGTGAGCAATGTTAAGCAGGTCAAGCAACAAGAAAGCCAAGAATGGCTTCTAT
TTGTCCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTAGCTTTTGGACTATCAAGTGCTACTGATTTTCGAAAAACAAATGCTGACGGTCGAGGCTACCAACCAAT
GCTTTTCAATGTAAAAGCTAGTACAATTAGTCAAAATAACCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

58.156

97.241

0.566

  comYF Streptococcus mutans UA159

57.447

97.241

0.559

  comGF/cglF Streptococcus pneumoniae Rx1

50.704

97.931

0.497

  comGF/cglF Streptococcus pneumoniae D39

50.704

97.931

0.497

  comGF/cglF Streptococcus pneumoniae R6

50.704

97.931

0.497

  comGF/cglF Streptococcus pneumoniae TIGR4

50.704

97.931

0.497

  comGF/cglF Streptococcus mitis SK321

47.917

99.31

0.476

  comGF/cglF Streptococcus mitis NCTC 12261

47.917

99.31

0.476

  comGF Lactococcus lactis subsp. cremoris KW2

44.604

95.862

0.428