Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V3H56_RS23095 Genome accession   NZ_CP144763
Coordinates   5239693..5241393 (+) Length   566 a.a.
NCBI ID   WP_338473906.1    Uniprot ID   -
Organism   Pseudomonas sp. MS646     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5234693..5246393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V3H56_RS23075 (V3H56_23075) - 5234940..5237873 (+) 2934 WP_338473905.1 DUF748 domain-containing protein -
  V3H56_RS23080 (V3H56_23080) - 5237875..5238171 (+) 297 WP_042730997.1 DUF2845 domain-containing protein -
  V3H56_RS23085 (V3H56_23085) - 5238437..5238790 (-) 354 WP_042730996.1 BON domain-containing protein -
  V3H56_RS23090 (V3H56_23090) - 5239055..5239462 (-) 408 WP_042730995.1 pilin -
  V3H56_RS23095 (V3H56_23095) pilB 5239693..5241393 (+) 1701 WP_338473906.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V3H56_RS23100 (V3H56_23100) pilC 5241396..5242613 (+) 1218 WP_042730993.1 type II secretion system F family protein Machinery gene
  V3H56_RS23105 (V3H56_23105) pilD 5242615..5243484 (+) 870 WP_042730992.1 A24 family peptidase Machinery gene
  V3H56_RS23110 (V3H56_23110) coaE 5243497..5244120 (+) 624 WP_338473907.1 dephospho-CoA kinase -
  V3H56_RS23115 (V3H56_23115) yacG 5244117..5244323 (+) 207 WP_042730990.1 DNA gyrase inhibitor YacG -
  V3H56_RS23120 (V3H56_23120) - 5244320..5244535 (-) 216 WP_042730989.1 hypothetical protein -
  V3H56_RS23125 (V3H56_23125) - 5244599..5245285 (-) 687 WP_042730988.1 energy-coupling factor ABC transporter permease -
  V3H56_RS23130 (V3H56_23130) - 5245453..5246079 (+) 627 WP_003185538.1 DUF1780 domain-containing protein -
  V3H56_RS23135 (V3H56_23135) - 5246201..5246374 (+) 174 WP_003185543.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62631.55 Da        Isoelectric Point: 5.7381

>NTDB_id=934808 V3H56_RS23095 WP_338473906.1 5239693..5241393(+) (pilB) [Pseudomonas sp. MS646]
MNDIALSGLTKQLVLAELITEQNAQQAYQQAQRNRMPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKENQPAGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESDDDRKQDTLAGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRVRTDGMLREVAKPPTQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKNKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKALYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNSRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKKALEIPDETLLKEGFPR
ERIGSFTIYEPVGCQQCNSGYKGRVGVYEVVRNTPQLQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=934808 V3H56_RS23095 WP_338473906.1 5239693..5241393(+) (pilB) [Pseudomonas sp. MS646]
ATGAATGACATCGCACTCAGCGGCTTGACCAAGCAACTGGTGTTGGCTGAACTGATCACCGAGCAGAATGCCCAGCAGGC
GTACCAGCAGGCCCAGCGCAATCGCATGCCCCTGGTCAGCTACCTGGTACAGAACAAACTGGTCCAGAGTCGTCAGGTTG
CGGAAATTGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGATCTCAACAGCCTCGACAAGGAGAACCAGCCCGCCGGCCTG
GTCAGCGAAAAACTGGTCCGCCAACACCACGCCTTGCCGCTCTGGCGACGTGGCAACAAGTTGTTCGTGGGCATCTCCGA
TCCAACCAACCACCAGGCCATCAATGACATTCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AACTCAGCGACGCCATCGAAAAGTTCTTCGAGTCCAGCAGCACCGGCCTGGAGGGCATGGGTGATGTCGACCTCGACGGC
CTGGACATCGAGTCGGACGACGATCGCAAGCAGGACACCCTTGCTGGCCAGGACGCCGACGACGCGCCGGTGGTGCGGTT
CGTCAACAAGATGTTGCTGGACGCGATCAAGGGCGGTTCCTCCGACCTGCACTTCGAGCCGTATGAAAAATCCTATCGGG
TGCGGGTGCGCACCGACGGCATGCTACGTGAAGTGGCCAAGCCGCCCACCCAACTGGCCGGCCGTATCGCCGCACGGCTG
AAGGTCATGGCCAGCCTGGACATTTCCGAGCGCCGCAAGCCCCAGGACGGACGCCTGAAAATGCGCCTGTCGAAAAACAA
GTCGATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTCATCCGGATCCTCGACCCTTCCAGCG
CGCAAATGGGCATCGACGCCCTGGGCTACGAGCCCGACCAGAAAGCGCTGTACATGGCGGCGCTCAAGCAACCCCAGGGG
CTGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACCGTATCGCTCTACACCGGGCTGAACATTCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAGGATCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAACTCGCGCCAGG
GGCTGGATTTCGCCCAGGCCCTGCGCTCTTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACGCTGCACACCAACAGCGCGGCGGA
AACCCTGATCCGCTTGCAGAACATGGGCATCCCCGGCTTCAACATCGCCACCGCCGTGCACTTGATCATCGCCCAGCGGC
TGGCGCGCAAGTTGTGCAACCACTGCAAGAAAGCCCTCGAGATTCCCGACGAAACCCTGCTCAAGGAAGGCTTCCCCCGG
GAACGCATCGGCTCATTCACGATCTATGAGCCGGTCGGTTGCCAGCAGTGCAACAGTGGCTACAAAGGGCGCGTGGGGGT
CTACGAAGTGGTCAGGAATACCCCTCAGTTGCAACGCCTGATCATGGCCGAAGGCAACTCGTTGGAAATCGACCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGCACCTCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Acinetobacter baumannii D1279779

57.143

100

0.572
  pilB Legionella pneumophila strain ERS1305867

54.497

100

0.546
  pilB Vibrio cholerae strain A1552

50.177

100

0.502
  pilF Neisseria gonorrhoeae MS11

48.496

99.823

0.484
  pilB Vibrio campbellii strain DS40M4

47.788

99.823

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

48.639

97.35

0.473