Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VZ068_RS16040 Genome accession   NZ_CP144460
Coordinates   3828030..3829766 (-) Length   578 a.a.
NCBI ID   WP_259162348.1    Uniprot ID   A0AAU7P5M0
Organism   Xanthomonas sp. 10-10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3823030..3834766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZ068_RS16030 (VZ068_16035) - 3824049..3825662 (+) 1614 WP_349655855.1 HAMP domain-containing sensor histidine kinase -
  VZ068_RS16035 (VZ068_16040) pilR 3826018..3827412 (+) 1395 WP_349655856.1 sigma-54 dependent transcriptional regulator Regulator
  VZ068_RS16040 (VZ068_16045) pilB 3828030..3829766 (-) 1737 WP_259162348.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VZ068_RS16045 (VZ068_16050) - 3829907..3830506 (-) 600 WP_259162349.1 methionine biosynthesis protein MetW -
  VZ068_RS16050 (VZ068_16055) - 3830499..3831539 (-) 1041 WP_349655857.1 glycosyltransferase family 2 protein -
  VZ068_RS16055 (VZ068_16060) - 3831542..3832336 (-) 795 WP_259162353.1 glycosyltransferase -
  VZ068_RS16060 (VZ068_16065) - 3832341..3832646 (-) 306 WP_349655858.1 SMR family transporter -
  VZ068_RS16065 (VZ068_16070) - 3833066..3833482 (+) 417 WP_349655859.1 pilin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62706.83 Da        Isoelectric Point: 5.6039

>NTDB_id=933962 VZ068_RS16040 WP_259162348.1 3828030..3829766(-) (pilB) [Xanthomonas sp. 10-10]
MNSVVTANLVGITGIARRLVQDGALDEGAARAAMDQAATAKVPLPQWFSEKKLVTASQLAAANSVEFGMPLLDVSSFDPN
QNAIKLVSEELLQKFQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQAGNASFGASLG
DDDEEMGDLDVSSGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKAVLP
EHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=933962 VZ068_RS16040 WP_259162348.1 3828030..3829766(-) (pilB) [Xanthomonas sp. 10-10]
ATGAATAGCGTCGTCACCGCCAACTTGGTTGGCATCACGGGCATTGCCCGGCGGCTTGTGCAGGACGGTGCTCTGGATGA
AGGCGCGGCGCGTGCTGCGATGGATCAGGCCGCTACTGCCAAGGTCCCGTTGCCGCAATGGTTCTCGGAGAAGAAGCTGG
TGACCGCTTCGCAACTAGCAGCTGCCAACTCGGTGGAGTTCGGGATGCCGCTACTTGATGTGTCATCGTTCGATCCTAAT
CAGAATGCAATCAAGTTGGTTAGCGAAGAATTGCTGCAGAAGTTTCAAGTGCTGCCCCTGTTCAAGCGCGGTAACCGGTT
ATTTGTGGGGGTGAGCAATCCGACACAGACTAGGGCCCTGGATGACATCAAGTTCCATACGAACCTGGTGGTTGAGCCAA
TTCTGGTTGACGAGGATCAGATCCGTCGAACCTTGGAGCAGTGGCAAGCAGGTAATGCGTCTTTTGGCGCGTCACTTGGT
GACGATGACGAAGAAATGGGAGACCTGGACGTCTCGTCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCGAAAGGCGACGACACGCCGGTGGTGAAGTTCGTCAATAAGGTGCTGGTGGATGCGATACGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCATACGAAGACGATTACCGGGTGCGCTTGCGCATCGACGGGCTGTTGAAGAACGTGGCCAAGGCACCG
GTGAAGCTGAGCCAGCGCATCGCCGCGCGTCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGGCAGTGCGGCCAAGCTGGGGATCGAGAAACTGGGCTACGAAGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAACCTTACGGCATGGTGCTGGTGACGGGGCCAACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTGAACGATGAGACGCGCAACATCTCAACTGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GCGTCAACCAGGTCCAGCAGAACAACAAACGCGGCATGACCTTTGCGGCGGCCCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCGACGCTGCATACCAACGATGCGCCACAGACCATCGCGCGTTTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGCCGCCTATGCAACAACTGCAAGCGCAAGGCGGTGTTGCCC
GAGCATGCGTTGTTGGCCGAAGGCTTCACGCCGGCGCAGATCGCTGCCGGTATCGAGCTGTATGAAGCGGTGGGCTGCGA
CGAATGCACCGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGC
TGGAAGGTGGCAACGCGATGCAGATCGCTGAGGCCGCCCAGAAGATCGGCATCAGGGATTTACGTCAGTCGGCGTTGGTC
AAAGCCGCGCATGGAGTGACTAGTCTGGCCGAGATCAATCGCGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.634

98.27

0.547
  pilB Legionella pneumophila strain ERS1305867

51.845

98.443

0.51
  pilB Vibrio cholerae strain A1552

50.906

95.502

0.486
  pilF Neisseria gonorrhoeae MS11

48.944

98.27

0.481
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

97.578

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

36.617

100

0.386