NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	V3829_RS01855	Genome accession	NZ_CP144358
Coordinates	378891..379505 (-)	Length	204 a.a.
NCBI ID	WP_041482377.1	Uniprot ID	-
Organism	Bacillus velezensis strain DJ4
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 373891..384505

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V3829_RS01825	rpsT	374190..374456 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
V3829_RS01830	holA	374473..375514 (-)	1042	Protein_364	DNA polymerase III subunit delta	-
V3829_RS01835	-	375554..375708 (-)	155	Protein_365	hypothetical protein	-
V3829_RS01840	-	375749..375883 (+)	135	WP_003152870.1	YqzM family protein	-
V3829_RS01845	-	375902..378254 (-)	2353	Protein_367	DNA internalization-related competence protein ComEC/Rec2	-
V3829_RS01850	-	378255..378824 (-)	570	WP_003152868.1	ComE operon protein 2	-
V3829_RS01855	comEA	378891..379505 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
V3829_RS01860	comER	379564..380385 (+)	822	WP_014305449.1	late competence protein ComER	-
V3829_RS01865	-	380454..381191 (-)	738	WP_032856885.1	class I SAM-dependent DNA methyltransferase	-
V3829_RS01870	rsfS	381188..381544 (-)	357	WP_003152864.1	ribosome silencing factor	-
V3829_RS01875	yqeK	381562..382122 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
V3829_RS01880	-	382112..382681 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
V3829_RS01885	yhbY	382692..382982 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
V3829_RS01890	aroE	382976..383818 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22222.38 Da Isoelectric Point: 6.0523

                                >NTDB_id=933702 V3829_RS01855 WP_041482377.1 378891..379505(-) (comEA) [Bacillus velezensis strain DJ4]

MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=933702 V3829_RS01855 WP_041482377.1 378891..379505(-) (comEA) [Bacillus velezensis strain DJ4]

ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG

CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363

Detailed information NTG0933702