Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V3Z63_RS28065 Genome accession   NZ_CP144289
Coordinates   5885806..5886330 (-) Length   174 a.a.
NCBI ID   WP_331708799.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CH1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 5884854..5885834 5885806..5886330 flank -28


Gene organization within MGE regions


Location: 5884854..5886330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V3Z63_RS28060 (V3Z63_28060) - 5884854..5885834 (-) 981 WP_003117291.1 IS5-like element ISPa41 family transposase -
  V3Z63_RS28065 (V3Z63_28065) pilA 5885806..5886330 (-) 525 WP_331708799.1 pilin Machinery gene

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 18610.33 Da        Isoelectric Point: 7.1673

>NTDB_id=933453 V3Z63_RS28065 WP_331708799.1 5885806..5886330(-) (pilA) [Pseudomonas aeruginosa strain CH1]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVTIKDTGDGTVKFTFATGQSSPKNAGKEITLNRTAEGVWTCTSTQEEMFIPKESPNKPSVMRESPQH
LIRAAHEPDELFRL

Nucleotide


Download         Length: 525 bp        

>NTDB_id=933453 V3Z63_RS28065 WP_331708799.1 5885806..5886330(-) (pilA) [Pseudomonas aeruginosa strain CH1]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAAGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGAATCTCCGAACAAGCCTTCAGTTATGCGAGAATCCCCGCAACAC
CTGATCCGAGCCGCCCATGAGCCAGATGAGCTTTTCCGACTTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

66.216

85.057

0.563
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.304

90.805

0.402
  pilA Vibrio cholerae strain A1552

44.304

90.805

0.402
  pilA Vibrio cholerae C6706

44.304

90.805

0.402
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.575

83.908

0.391
  pilA Acinetobacter baumannii strain A118

44.521

83.908

0.374