Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   V3435_RS05315 Genome accession   NZ_CP144270
Coordinates   1100108..1101178 (-) Length   356 a.a.
NCBI ID   WP_000776425.1    Uniprot ID   -
Organism   Staphylococcus aureus strain LA52     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1095108..1106178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V3435_RS05275 (V3435_05265) gcvT 1095318..1096409 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  V3435_RS05280 (V3435_05270) - 1096568..1097092 (-) 525 WP_001015120.1 shikimate kinase -
  V3435_RS05285 (V3435_05275) - 1097082..1097228 (-) 147 WP_001789879.1 hypothetical protein -
  V3435_RS05290 (V3435_05280) - 1097295..1098467 (-) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  V3435_RS05295 (V3435_05285) comGF 1098658..1099155 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  V3435_RS05300 (V3435_05290) comGE 1099073..1099372 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  V3435_RS05305 (V3435_05295) comGD 1099359..1099805 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  V3435_RS05310 (V3435_05300) comGC 1099783..1100094 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  V3435_RS05315 (V3435_05305) comGB 1100108..1101178 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  V3435_RS05320 (V3435_05310) comGA 1101150..1102124 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  V3435_RS05325 (V3435_05315) - 1102176..1102799 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  V3435_RS05330 (V3435_05320) - 1102796..1103125 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  V3435_RS05335 (V3435_05325) - 1103125..1104111 (-) 987 WP_000161315.1 ROK family glucokinase -
  V3435_RS05340 (V3435_05330) - 1104108..1104311 (-) 204 WP_000087559.1 YqgQ family protein -
  V3435_RS05345 (V3435_05335) - 1104292..1105755 (-) 1464 WP_001017856.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41489.97 Da        Isoelectric Point: 10.1030

>NTDB_id=933110 V3435_RS05315 WP_000776425.1 1100108..1101178(-) (comGB) [Staphylococcus aureus strain LA52]
MKLQWINTFKLHSKKRQLSKVQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=933110 V3435_RS05315 WP_000776425.1 1100108..1101178(-) (comGB) [Staphylococcus aureus strain LA52]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGTCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTCTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGTTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994