NTDB

Overview

Name	pilE	Type	Machinery gene
Locus tag	ACDW35_RS25280	Genome accession	NZ_CP167983
Coordinates	5440959..5441498 (-)	Length	179 a.a.
NCBI ID	WP_003141352.1	Uniprot ID	A0A0H2ZFW8
Organism	Pseudomonas aeruginosa strain 0102d1_7
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5435959..5446498

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACDW35_RS25250 (ACDW35_25250)	-	5436041..5436271 (+)	231	WP_034055169.1	helix-turn-helix domain-containing protein	-
ACDW35_RS25255 (ACDW35_25255)	-	5436386..5436598 (+)	213	WP_023109351.1	hypothetical protein	-
ACDW35_RS25260 (ACDW35_25260)	-	5436601..5438442 (+)	1842	WP_034055171.1	ClpP-like prohead protease/major capsid protein fusion protein	-
ACDW35_RS25265 (ACDW35_25265)	-	5439712..5439954 (+)	243	WP_023109353.1	hypothetical protein	-
ACDW35_RS25275 (ACDW35_25275)	-	5440197..5440895 (-)	699	WP_016254081.1	hypothetical protein	-
ACDW35_RS25280 (ACDW35_25280)	pilE	5440959..5441498 (-)	540	WP_003141352.1	pilin	Machinery gene
ACDW35_RS25285 (ACDW35_25285)	pilB	5441727..5443430 (+)	1704	WP_003141354.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACDW35_RS25290 (ACDW35_25290)	pilC	5443434..5444651 (+)	1218	WP_016254080.1	type II secretion system F family protein	Machinery gene
ACDW35_RS25295 (ACDW35_25295)	pilD	5444654..5445526 (+)	873	WP_003141356.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
ACDW35_RS25300 (ACDW35_25300)	coaE	5445523..5446134 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
ACDW35_RS25305 (ACDW35_25305)	yacG	5446131..5446331 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 179 a.a. Molecular weight: 18083.45 Da Isoelectric Point: 5.0804

                                >NTDB_id=933098 ACDW35_RS25280 WP_003141352.1 5440959..5441498(-) (pilE) [Pseudomonas aeruginosa strain 0102d1_7]

MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT

SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASESNAVSSGPDRN

MPALTAGTLPARFAPSECR

Nucleotide

Download Length: 540 bp

                                >NTDB_id=933098 ACDW35_RS25280 WP_003141352.1 5440959..5441498(-) (pilE) [Pseudomonas aeruginosa strain 0102d1_7]

ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT

GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG

GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC

AGTAAGTATGTGACCTCTGTGCGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA

TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT

ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT

ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H2ZFW8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	42.64	100	0.469
pilA	Ralstonia pseudosolanacearum GMI1000	43.243	100	0.447
pilA2	Legionella pneumophila str. Paris	41.808	98.883	0.413
pilA2	Legionella pneumophila strain ERS1305867	41.243	98.883	0.408
comP	Acinetobacter baylyi ADP1	38.333	100	0.385
pilE	Neisseria gonorrhoeae strain FA1090	35.714	100	0.363

Detailed information NTG0933098