Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V3C05_RS11540 Genome accession   NZ_CP143878
Coordinates   2350023..2350457 (+) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. multocida strain LH06     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2345023..2355457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V3C05_RS11510 (V3C05_11510) tadA 2345134..2345634 (-) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -
  V3C05_RS11515 (V3C05_11515) - 2345649..2346500 (-) 852 WP_330962086.1 thymidylate synthase -
  V3C05_RS11520 (V3C05_11520) lgt 2346497..2347309 (-) 813 WP_010906471.1 prolipoprotein diacylglyceryl transferase -
  V3C05_RS11525 (V3C05_11525) - 2347319..2348113 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  V3C05_RS11530 (V3C05_11530) rppH 2348116..2348712 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  V3C05_RS11535 (V3C05_11535) ampD 2349297..2349863 (-) 567 WP_005751074.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V3C05_RS11540 (V3C05_11540) pilA 2350023..2350457 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  V3C05_RS11545 (V3C05_11545) pilB 2350457..2351842 (+) 1386 WP_010906472.1 GspE/PulE family protein Machinery gene
  V3C05_RS11550 (V3C05_11550) - 2351842..2353053 (+) 1212 WP_005722560.1 type II secretion system F family protein -
  V3C05_RS11555 (V3C05_11555) - 2353053..2353775 (+) 723 WP_010906474.1 prepilin peptidase -
  V3C05_RS11560 (V3C05_11560) coaE 2353790..2354410 (+) 621 WP_010906475.1 dephospho-CoA kinase -
  V3C05_RS11565 (V3C05_11565) yacG 2354421..2354624 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  V3C05_RS11570 (V3C05_11570) - 2354624..2354893 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=931660 V3C05_RS11540 WP_005741096.1 2350023..2350457(+) (pilA) [Pasteurella multocida subsp. multocida strain LH06]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=931660 V3C05_RS11540 WP_005741096.1 2350023..2350457(+) (pilA) [Pasteurella multocida subsp. multocida strain LH06]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCTGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACAGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368